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Citations for Tocris :
301 - 350 of 403 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with either DMSO or 1 µM of LRRK2 kinase inhibitor (MLi-2, cat.no. 5756, TOCRIS) for 90 mins and then examined for localization ...
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bioRxiv - Cancer Biology 2021Quote: ... Briefly,15,000 cells were incubated at 37°C with or without 10 µM of the A740003 (Tocris, UK) for 1 hour in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Cell Biology 2020Quote: ... Logarithmically proliferating HeLa Kyoto cells were treated with Eg5 inhibitor 7.5 μM S-Trityl-L-cysteine (Tocris Biosciences) for 18 h ...
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bioRxiv - Genomics 2019Quote: ... 20ng/ml human LIF (all from WT-MRC Cambridge Stem Cell Institute) and 2 μM Gö6983 (PKCi; Tocris) on Matrigel–coated plates (Corning) ...
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bioRxiv - Immunology 2020Quote: ... Inhibitors of cell signalling were added 30 min prior to cytokine stimulation: BIRB796 (10µM, p38 MAPK inhibitor, Tocris), PD98059 (50µM ...
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bioRxiv - Genetics 2022Quote: ... cells were incubated for 16 h with 250 nM mTOR inhibitor Torin 1 (4247, TOCRIS Bioscience, Bristol, UK) and 4 h with 200 nM bafilomycin A1 ...
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bioRxiv - Molecular Biology 2022Quote: Parental A549 cells were fixed with 4% paraformaldehyde following 3-hour treatment with poly I:C (2.0 μg/mL, Tocris), LPS (1.0 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... with stem cells cultured in serum-free floating embryoid body-like aggregates with 1mM of dorsomorphin (Tocris, 3093), 2 mM of A83-01 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... to create a single cell suspension prior to resuspension in TeSR medium supplemented with ROCK kinase inhibitor (Tocris 1254 ...
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bioRxiv - Genomics 2023Quote: ... and seeded at a seeding density of 10,000–25,000 cells/cm2 in mTeSR supplemented with 2 μM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mL fresh RPMI 1640 containing B-27 supplement minus insulin was added to 0.5 mL conditioned (old) medium and added to cells along with 5 µM IWP2 (Tocris). Medium was aspirated at Day 5 and replenished with fresh RPMI 1640 containing B-27 supplement minus insulin ...
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bioRxiv - Cell Biology 2020Quote: ... Electroporated cells were seeded in fresh E8 media containing 5 μM Y-27632 dihydrochloride (a selective ROCK inhibitor, Tocris) and allowed to recover for 48 hours before antibiotic selection.
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bioRxiv - Developmental Biology 2020Quote: ... and seeding onto fresh MEFs with 10 μM Y27632 ROCK inhibitor overnight to increase cell survival (Tocris Bioscience, UK).
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bioRxiv - Molecular Biology 2021Quote: ... cells were pre-treated with 10 μM of Poly (ADP-ribose) glycohydrolase inhibitor (PARGi, TOCRIS Bio-Techne, PDD 00017273) for 30 mins prior to IR exposure ...
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bioRxiv - Neuroscience 2021Quote: ... Where indicated cells were pre-incubated with a nitric oxide donor (S)-Nitroso-N-acetylpenicillamine (SNAP, 1000 μM, Tocris) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Whole-cell voltage-clamp recordings for mEPSC and mIPSC were conducted in the presence of TTX (1 μM; Tocris) and Picrotoxin (100 uM ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in E8 medium supplemented with 10 µM ROCK inhibitor Y-27632 dihydrochloride (Tocris, Bristol, United Kingdom) and seeded on Matrigel-coated 12-well plates at a density of (1.5–2.5)×104 cells/cm2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Depletion of endogenous proteins fused to the 2xHA-FKBP12F36V domain (dTAG) was achieved by treating the cells with 0.5 μM dTAG-13 compound (Tocris, 1:1000 dilution of 0.5 mM stock solution in DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... hESCs were replated onto 35 mm Matrigel-coated plates at a density of 1.5 x 105 cells/cm2 in E8 medium with 10 μM ROCK inhibitor (Y27632, Tocris). The media was replaced with Essential 6 (E6 ...
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bioRxiv - Cancer Biology 2020Quote: ... 70% confluent transduced cells were treated with 1μM of the positive CaSR modulator NPS R-568 (Tocris Bioscience, UK) or the negative CaSR modulator NPS 2143 (Tocris Bioscience ...
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bioRxiv - Bioengineering 2019Quote: ... cells were re-plated onto Matrigel-coated plates at a density of 2.5×105 cells/cm2 in E8 medium containing 10 μM Y27632 (Tocris). The following day ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cell cultures were treated with Accutase and replated at a density of 63,000 cells//cm2 in N2B27 supplemented with CHIR99021 (3 µM, Tocris), FGF2 (100 ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then differentiated for 3 days (D) in N2B27 supplemented with 3 µM Chiron99021 (Tocris, Cat. No. 4953) and 20ng/ml bFGF (PeproTech ...
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bioRxiv - Genetics 2021Quote: ... Cells were then incubated in RPMI/B27 (-Insulin) for 24 hours before changing media to 5 μM IWP2 (Tocris) in RPMI/B27(-Insulin) ...
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bioRxiv - Cell Biology 2021Quote: ... organoids cultured for 8 days with airway cell culture medium were followed by inclusion of CHIR99021 (2 µM, Tocris) for additional 10 days with or without DAPT (20 µM) ...
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bioRxiv - Developmental Biology 2022Quote: ... the cells were washed three times in 0.1 % Triton X-100/PBS (for nuclei staining 1 μg/mL DAPI (Tocris) was added to the first wash ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded onto Matrigel-coated plates at a density of 15,800 cells/cm2 in E8 medium containing 10 µM Y27632 (Tocris). The next day ...
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bioRxiv - Neuroscience 2022Quote: ... Matured iMGLs at density of 15 000 cells/well on 96-well plates were treated with 0.3-50 µM Yoda1 (Tocris) or equal volume of DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 1 uM of the microtubule-stabilizing agent SAHA (TOCRIS bioscience Cat. No. 149647-78-9) for 30 minutes before activation or experimental procedures ...
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bioRxiv - Cell Biology 2023Quote: For inhibition of P2X4R cells were pre-incubated for 30 min with 10 μM of 5-BDBD (Tocris Bioscience). Blockage of αvβ3/αvβ5 integrins was achieved by the pre-incubation with 10 μM of cilengitide for 2 hours (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... 3- (4,5-dimmethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to estimate cell number following the manufacturer’s description (Tocris, # 5224), and absorbance was measured at 550 nm in an EnsightTM 96-well plate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in Anteriorization media-1 (100 ng/ml Noggin (R&D/Biotechne) and 10 μM SB431542 (Tocris)) for 24 hours ...
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bioRxiv - Genetics 2023Quote: ... cells underwent various drug experiments: for cAMP analogs (8Br-cAMP, db-cAMP, and 6Bnz-cAMP, Tocris Bioscience, Bristol, UK), they were incubated for 48 hours with the appropriate concentrations (50 and 500µM for 8Br-cAMP and db-cAMP ...
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bioRxiv - Neuroscience 2024Quote: ... mEPSC recordings were obtained from cells voltage-clamped at –70 mV in ACSF containing 0.5 µM tetrodotoxin (TTX, Tocris) and 50 µM picrotoxin (PTX ...
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bioRxiv - Neuroscience 2024Quote: Myelin degeneration was obtained by treating myelinated Schwann cell–DRG neuronal co-cultures with 25 μM AT–56 (Tocris) in myelinating medium for additional 7 days ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 12-well plates at the density of 6×105 cells/well in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, Tocris). The medium was changed daily ...
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bioRxiv - Neuroscience 2019Quote: ... The chemical inhibition of CTL6 cells was conducted by a global perfusion of clozapine-n-oxide (CNO, 5uM, Cat# 4936, Tocris), the chemical actuator of the chemogenetic probe ...
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bioRxiv - Neuroscience 2020Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...
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bioRxiv - Cell Biology 2021Quote: ... flow cytometer, and the fractions of stained cells were quantified using FloJo 10.6, with Staurosporine (4 h, 5 μM) (Tocris, 1285) used as a positive control for cell death and to help establish gating of the sorted cells.
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bioRxiv - Developmental Biology 2022Quote: ... iPSCs were dissociated into single cells and plated at a density of 50,000 cells/well in a vitronectin-coated 24-well plate with 10µM of the ROCK inhibitor Y-27632 (Tocris Bioscience). The next day ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were either untreated (GFP-Apex and S1R-Apex) or treated (S1R-Apex) with 25μM Haloperidol (Tocris, Minneapolis, MN) or 20μM (+)-PTZ (Millipore-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... cells were rinsed with ice cold PBS and then collected and suspended by scraping in PBS containing 50nM JZL195 (Tocris), a dual inhibitor of FAAH and MAGL (Long et al. ...
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bioRxiv - Cell Biology 2020Quote: ... definitive endoderm cells were generated from hESCs (∼90% confluence) by the combination of 1-2µM CHIR-99021 (Tocris, 4423-10MG) [9] and 100ng/ml ActivinA (R&D Systems ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Cell Biology 2021Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 dissociated iPS cells resuspended in mTeSR1 media containing 10 µM Y-27362 dihydrochloride (ROCKi; Tocris Bioscience, cat. no. 1254) and 0.05% poly-vinyl alcohol (PVA ...
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bioRxiv - Genetics 2019Quote: ... cells were treated with phorbol 12-myristate 13-acetate (PMA; 100nM in DMSO) or DMSO or PKC antagonist (GF10930X; Tocris) for 24 hours following which cells were lysed for dual luciferase analysis as per manufacturer’s instructions (Promega UK).
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bioRxiv - Developmental Biology 2020Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...