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Citations for Tocris :
251 - 300 of 1264 citations for Carboxypeptidase Y from Baker's Yeast since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 10 μM Y-27632 (ROCK inhibitor, Selleckchem, Houston, TX, USA) 500 nM A83-01 (Tocris Bioscience, Bristol, UK), 3 μM SB202190 (Sigma Aldrich) ...
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bioRxiv - Developmental Biology 2024Quote: ... and single cells were seeded with medium supplemented with 10 µM Y- 27632 (ROCK inhibitor; Tocris Biosciences, 1254). Fresh medium was exchanged daily ...
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bioRxiv - Cell Biology 2024Quote: ... was added to iPSCs after thawing to improve recovery and ROCK inhibitor Y-27632 (10 µM, Tocris, 1254) was added occasionally after passaging to improve viability ...
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bioRxiv - Cell Biology 2020Quote: ... Electroporated cells were seeded in fresh E8 media containing 5 μM Y-27632 dihydrochloride (a selective ROCK inhibitor, Tocris) and allowed to recover for 48 hours before antibiotic selection.
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bioRxiv - Bioengineering 2020Quote: ... at 37°C for 5 min and then seeding at 1:10 to 1:20 ratio with addition of 10μM of the ROCK inhibitor Y-27632 (Tocris). The E8 maintenance medium was changed every day.
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bioRxiv - Neuroscience 2021Quote: ... The resulting cell pellet was broken up and resuspended in 1ml E8 media by 1000ul pipette and distributed on a Geltrex coated 6 well plate with E8 and 10µM ROCK inhibitor (Y-27632 dihydrochloride, Tocris) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in E8 medium supplemented with 10 µM ROCK inhibitor Y-27632 dihydrochloride (Tocris, Bristol, United Kingdom) and seeded on Matrigel-coated 12-well plates at a density of (1.5–2.5)×104 cells/cm2 ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 2.5*106 per 10 cm dish (Falcon) in StemFlex supplemented with 10 µM Y-27632 (Tocris) on day −2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were passaged every 6 days and kept in a cycle of 3 days in 5μM ROCK inhibitor (Y-27632 dihydrochloride; Tocris), 3 days in the absence of rock inhibitor ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pellets were resuspended in a fresh media supplemented with 10 µM ROCK inhibitor Y-27632 (Tocris Bioscience #1254) for 24 hours to promote the survival of the single cells ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.1 million H9 or RUES2 hPSCs were plated in E8 medium with 10 μM Rock inhibitor Y-27632 (Tocris) in 1 well of 12-well culture plates coated with iMatrix-511 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... 3M cells on the second day of bFGF treatment were plated onto the chips in N2B27 medium with 10ng/ml bFGF + 10µM Y-27632 ROCK inhibitor (Tocris) and washed 3h after plating.
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated from the 100 mm dishes and 2.8-3M cells per well were seeded on the prepared 2-well dishes in N2B27 medium with 10ng/ml bFGF + 10µM Y-27632 ROCK inhibitor (Tocris) media for 3h ...
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bioRxiv - Developmental Biology 2023Quote: ... For passaging TESR medium was further supplemented with 10 μM Rho kinase inhibitor Y-27632 (Tocris, Cat. No. 1254).
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bioRxiv - Cell Biology 2024Quote: ... Samples were further digested into single cells in 0.2% trypsin in PBS supplemented with 10 µmol/mL Y-27632 (Tocris). The digestion was stopped with EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... Organoids were recovered by quick thaw in a 37C water bath followed by washing in organoid media and plating in ECM with organoid media plus 10 μM ROCK inhibitor Y-27632 (Tocris).
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bioRxiv - Genetics 2021Quote: ... which were resuspended in mTeSR Plus media with 10 µM Rho-associated protein kinase inhibitor (ROCKi, Tocris Bioscience #Y-27632) for 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... coated 12-well plates at the density of 6×105 cells/well in mTeSR1 medium supplemented with 10 μM ROCK inhibitor (Y-27632, Tocris). The medium was changed daily ...
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bioRxiv - Developmental Biology 2022Quote: ... iPSCs were dissociated into single cells and plated at a density of 50,000 cells/well in a vitronectin-coated 24-well plate with 10µM of the ROCK inhibitor Y-27632 (Tocris Bioscience). The next day ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells embedded in matrigel were incubated at 37C for 15 minutes and covered with PaTOM media supplemented with Y-27632 dihydrochloride (Tocris)
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bioRxiv - Bioengineering 2020Quote: ... Biolamina)-coated 384-well plates in 30 μL of E8 media supplemented with CEPT cocktail or Y-27632 (10 μM, Tocris). After 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 dissociated iPS cells resuspended in mTeSR1 media containing 10 µM Y-27362 dihydrochloride (ROCKi; Tocris Bioscience, cat. no. 1254) and 0.05% poly-vinyl alcohol (PVA ...
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...
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bioRxiv - Neuroscience 2022Quote: ... 150 μL of complete activated media (treated with vehicle (H2O) or 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254)) was very carefully added to each well (for the ROCKi experiments) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were routinely passaged at a 1:8-1:10 split ratio in the presence of Y-27632 (ROCK inhibitor, Tocris) for the first day after passaging and removed for subsequent media changes.
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bioRxiv - Neuroscience 2022Quote: ... All iPSC lines were first seeded concurrently on GeltrexTM (Thermo)-coated plates at 105,000 cells/cm2 (1,000,000 cells in a well of a six-well plate) in mTeSRTM medium supplemented with 10 μM Y-27632 (Tocris). The Y-27632 compound was removed from the medium the next day and subsequently the medium was replaced daily ...
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bioRxiv - Cell Biology 2023Quote: iPSCs were first differentiated into iAT2s and passaged as above in 3D cultures in iAT2 medium (CK+DCI) supplemented for the first 3 days after passaging with 10 uM Y-27632 (Tocris). To generate iAT1s ...
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bioRxiv - Genetics 2023Quote: ... iPSC lines were fed complete E8 medium supplemented with Y-27632 dihydrochloride at final concentration of 10uM (Tocris Bioscience #1254). To create a 1000X stock ...
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bioRxiv - Developmental Biology 2023Quote: ... Mechanically dissociated cells in accutase were diluted with 4x volume of StemFlex with 10uM Y-27632 (ROCK inhibitor, Tocris, #1254) and manually counted with Trypan Blue ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded in 60 mm dishes coated with GFR Matrigel and incubated in mTESR1 with 10 μM Y-27632 Rock inhibitor (Tocris). At the time of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... MSD3 iPSCs were transduced with lentiviral constructs in mTeSR Plus medium containing 10 µM Y-27632 (ROCK inhibitor; Tocris, 1254) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... NN3053 hESCs were plated on Lam-inin-521 in mTeSR1 medium with 10 µM Rock inhibitor Y-27632 (Tocris Bioscience). The following day ...
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bioRxiv - Immunology 2024Quote: ... according to the manufacturer’s protocol in mTeSR PLUS was supplemented with penicillin/streptomycin and 10 µM ROCK inhibitor (Y-27632 Tocris, 1254). Three days later about 200 EB were transferred to a well of a 24-well ultra-low attachment plate (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... The drugs used were 10 µM para-Nitroblebbistatin (motorPharma, #DR-N-111) or 5 µM Y-27632 (Tocris Bioscience, #1254). As a control we used dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2024Quote: ... and plated on Geltrex coated 6 well plates at a density of 10,000 iPSCs/cm2 in 2ml/well of mTeSR1 + 10uM Y-27632 dihydrochloride (Tocris, 1254). Cells were cultured at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: RUES2-GLR hESCs grown routinely in NutriStem® hPSC XF were first plated as single cells at a density of 5.2-6.7 x 103 cells/cm2 with 5 µM Y-27632 (Tocris Biosciences, 1254) for the first 24h after plating ...
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bioRxiv - Cell Biology 2024Quote: ... coated 6-well plates with 1-1.5 million cells per well in E8 with 10 μM ROCK inhibitor Y-27632 (Tocris 1254). At day 1 of differentiation medium was exchanged to induction medium (IM ...
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bioRxiv - Immunology 2021Quote: ... the apical and basolateral chambers were fed PneumaCult ™ ALI media (StemCell #05021) supplemented with 10μM ROCK inhibitor (Tocris Y-27632). Upon reaching confluency ...
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bioRxiv - Cell Biology 2021Quote: ... hiPSCs were seeded on Matrigel-coated plates or coverslips at a density of 1.1×105 cells/cm² in mTeSR1 medium supplemented with 10 μM of ROCK inhibitor Y-27632 (Ri; Tocris, ref. 1254). To induce MES differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... the Versene solution was then aspirated and replaced with E8 + 10 nM Y-27632 dihydrochloride ROCK inhibitor (Tocris cat. no. 125410); hiPSC colonies were then gently detached mechanically using a cell scraper ...
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bioRxiv - Neuroscience 2022Quote: ... 12-well plates at 3-4 × 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y-27632; Tocris, 1254). Medium without the inhibitor was replaced daily until the cells reach 80-100% confluency ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension using accutase and seeded at a density of 1.0 x 106 cells per well on Matrigel coated 6-well plates in mTeSR1 supplemented with 10 mM ROCK inhibitor Y-27632 (Tocris, 1254).
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bioRxiv - Neuroscience 2021Quote: ... the Versene solution was then aspirated and replaced with E8 + 10 nM Y-27632 dihydrochloride ROCK inhibitor (Tocris cat. no. 125410); hiPSC colonies were then gently detached mechanically using a cell scraper ...
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bioRxiv - Neuroscience 2020Quote: ... hiPSC were passaged upon reaching approximately 80% confluence using EDTA at 37°C for three to five minutes and plated in mTESR1 with 10 μM Y-27562 (Tocris, UK). Media without Y-27562 was supplemented the day following.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... coated 6-well plates at a density of 100,000 cells/cm2 in E8 medium containing 10 μM ROCK-inhibitor (Y-27632 (Tocris, Bristol, UK)) ...
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bioRxiv - Developmental Biology 2022Quote: ... are seeded in a 24-well plate (TPP, #92024) at 30-40k cells per well in E8 + ROCKi (5 µM Y-27632, Tocris #1254). All differentiation media are based on CDM that consists of 5 mg/ml bovine serum albumin (Europa Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Human ePSCs were thawed at 37°C and plated on top of the feeder cells in EPSCM with 10 µM Y-27632 dihydrochloride (Tocris, 1254) for 48 h ...
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bioRxiv - Genetics 2022Quote: ... Medium was changed on the next day and GFP positive cells were sorted 48-72 h post transfection and plated on feeder cells in standard TSC medium containing 10 μM ROCK inhibitor Y-27632 (Tocris #1254). Clonal TSC colonies were picked 7-10 days post sort ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Neuroscience 2022Quote: Cultured aNSCs/NPCs from young and old male C57BL/6 mice were plated onto PDL-coated coverslips in 24-well plates with complete activated media with or without 10 μM ROCK inhibitor (ROCKi) compound Y-27632 (dissolved in H2O) (Tocris, 1254). Forty-eight hours after plating ...