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Citations for Tocris :
201 - 239 of 239 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Medium was changed on the next day and GFP positive cells were sorted 48-72 h post transfection and plated on feeder cells in standard TSC medium containing 10 μM ROCK inhibitor Y-27632 (Tocris #1254). Clonal TSC colonies were picked 7-10 days post sort ...
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bioRxiv - Neuroscience 2021Quote: ... d-2-Amino-5-phosphonovaleric acid (d-AP5, 50µM) and the sodium (Na+) channel blocker tetrodotoxin (TTX, 1 µM Tocris). Individual traces (5 sec duration ...
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bioRxiv - Neuroscience 2021Quote: ... In a subset of experiments the D1 receptor agonist (SKF81297; 20 mg/kg in saline; Tocris R&D systems), was injected intraperitoneally while recording cADDis photometry signals ...
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bioRxiv - Cell Biology 2021Quote: ... and 6-O-[(4,5-Dimethoxy-2-nitrophenyl)methyl]-2,3-O-(1-methylethylidene)-D-myo-Inositol 1,4,5-tris[bis[(1-oxopropoxy)methyl]phosphate] (ci-IP3/PM; 1 μM, Tocris #6210) in imaging buffer with 0.01 % BSA in dark at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then differentiated for 3 days (D) in N2B27 supplemented with 3 µM Chiron99021 (Tocris, Cat. No. 4953) and 20ng/ml bFGF (PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... (5S,10R)-(+)-5-Methyl-10,11-dihydro-5H-dibenzo[a,d] cyclohepten-5,10-imine maleate (MK-801, 10 μM dissolved in H2O; Tocris Bioscience), 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl ...
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bioRxiv - Bioengineering 2023Quote: ... 10 nM gastrin, 50 ng/ml recombinant human EGF (R&D, 236-EG) and 500 nM A83-01 (Tocris). Organoids were passaged every 4 days by physical dissociation using fire-polished Pasteur pipettes.
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated in Anteriorization media-1 (100 ng/ml Noggin (R&D/Biotechne) and 10 μM SB431542 (Tocris)) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and AMPAR currents were then isolated at +40mV by bath application of 10µM D-APV (NMDAR blocker, Tocris 0106). AMPA rectification indices were calculated as a ratio of isolated AMPAR ChR2-PSC peak at +40mV ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with FGF-2 (20 ng ml−1), Activin-A (50 ng ml−1), BMP-4 (10 ng ml−1, R&D) and LY294002 (10 mM Tocris). Media was changed again after 42 hours to CDM-BSA supplemented with FGF-2 (8 ng ml−1) ...
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bioRxiv - Neuroscience 2021Quote: ... Drug-evoked locomotor-like activities were induced by bath-applying 10 to 14 µM of N-methyl- D-aspartate (NMDA, Tocris) and serotonin (5-HT ...
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bioRxiv - Neuroscience 2020Quote: ... Intrinsic properties were recorded in the presence of 10 μM biccuculine methiodide (Enzo, Life Sciences) and 10 μM saclofen (R&D, Tocris). We studied only cells with series resistance < 25 MΩ ...
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bioRxiv - Developmental Biology 2020Quote: ... day 3 NMP-like cultures were treated with Accutase and replated at a density of 45,000 cells/cm2 on Geltrex-coated plates in N2B27 containing FGF2 (40 ng/ml, R&D) and CHIR99021 (8 µM, Tocris) for two days ...
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bioRxiv - Neuroscience 2020Quote: ... N-methyl D-aspartate (NMDA) receptors were blocked by the non-competitive NMDA receptor antagonist MK-801 (Tocris; 100 μM), co-applied with the competitive α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid AMPA/kainate receptor antagonist CNQX (Tocris ...
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bioRxiv - Cell Biology 2021Quote: ... The growth medium was supplemented with 100 ng/mL human recombinant epidermal growth factor (EGF) (R&D) as the major growth-supporting peptide and was further supplemented with 10 µM Y27632 (Tocris), 500 nM A83-01 (Tocris) ...
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bioRxiv - Microbiology 2022Quote: ... 3 μl of 10 mM of NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Tocris Bioscience) was added to the resuspension ...
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bioRxiv - Cancer Biology 2022Quote: ... and plated at 60,000 cells/cm2 in N2B27 medium supplemented with 20 ng/ml of FGF2 (Cat# 233-FB/CF, R&D) and 4 μM of CHIR 99021 (Cat# 4423, Tocris) and 10 μM of Rock Inhibitor (Y-27632 ...
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bioRxiv - Cell Biology 2023Quote: ... The indicated drugs were directly added in the culture medium: torin-1 (10 μM) (4247/10, R&D and Tocris), Rotenone (500 nM ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 25 µl PBS together with 0.25 µl of 5 mM 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl) carbonyl]amino]-D-alanine hydrocholoride (HADA) (Tocris) dissolved in DMSO ...
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bioRxiv - Physiology 2021Quote: ... Picrotoxin (100 µM) was used to block GABAA-receptor-mediated currents and D-2-amino-5-phosphonovalerate (APV; 20 µMm, Tocris Bioscience), and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX ...
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bioRxiv - Cell Biology 2021Quote: ... B/D medium was supplemented during submerged culture with 1 nM EC23 (light-stable retinoic acid receptor agonist; Tocris, Abingdon, UK). After confluence was reached ...
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bioRxiv - Cell Biology 2021Quote: ... 4 × 106 cells were seeded in a 21 cm2 dish and after 24 hours stimulated for ten minutes with 10 μM DAMGO ([D-Ala2, N-MePhe4, Gly-ol]-enkephalin, Tocris 1171) in DMEM medium (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with 100 ng/mL Activin A (R&D, Minneapolis, MN, USA) and 3–5 µM CHIR99021 (Tocris Bioscience, Bristol, UK) (differentiation day 0 ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 50 μM D,L-2-amino-5-phosphonovaleric acid (AP5) (both from Tocris, Bristol, UK) to block recurrent glutamatergic signaling.
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bioRxiv - Neuroscience 2022Quote: ... Intervet AB, Sweden), PCP (phencyclidine hydrochloride, 5 mg/kg, Lipomed AG, Switzerland) or amphetamine (d-amphetamine hydrochloride, 4 mg/kg, Tocris, UK) and recorded for another 60-120 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... all buffers and solutions used during the process (from animal perfusion to sorted cells flash freezing) were supplemented with a cocktail composed of Actinomycin D (3μM, Tocris #1229/10), Anisomycin (100 μM ...
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bioRxiv - Neuroscience 2024Quote: ... All recordings were conducted in the presence of glutamatergic receptor blockers – 50µM D-AP5 (Tocris Cat#0106 and HelloBio Cat#H0225), and 5µM NBQX (Tocris Cat#1044 and HelloBio Cat#HB0443 ...
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bioRxiv - Neuroscience 2024Quote: ... along with an ICV cannula in mice to deliver either CXCL10 (R&D, 266-IP/CF) alone or in a combination of AMG487 (Tocris, 4487). To do this ...
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bioRxiv - Neuroscience 2020Quote: ... containing 10 μM 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and 50 μM D,L-2-amino-5-phosphonovaleric acid (AP5) to block glutamatergic signaling (both from Tocris, Bristol, UK). Experiments involving tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced with HBSS containing 6-N,N-Diethyl-β-γ-dibromomethylene-D-adenosine-5′-triphosphate (ARL 67156; Tocris, 1283) at a concentration of 100 μM ...
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bioRxiv - Neuroscience 2022Quote: ... these experiments were performed in the presence of AMPA and NMDA receptor blockade (10 µM NBQX and 50 µM D-AP5 respectively; Tocris, Bristol, UK). To isolate excitatory currents ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614], 25 ng/mL SHH-C25II [R&D Systems, 464-SH-025], 250 nM Purmorphamine [Tocris, 4551] ...
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bioRxiv - Neuroscience 2023Quote: ... Drug treatments correspond to a single dose of cocaine hydrochloride at 20 mg/kg (Medisca, cat# 53-21-4, Canada) or D-amphetamine sulfate at 5 mg/kg (Tocris, 2813, UK) at doses known to increase locomotion87 ...
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bioRxiv - Neuroscience 2024Quote: ... or N-methyl-D-aspartic acid (NMDA) (final concentration 100 μM; 1 µL stock solution in PBS in 1 ml media 0114; Tocris, Abingdon, UK). A DMSO-only control was conducted for comparison with PTX ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was aspirated and the pellet re-suspended in fresh E8 medium supplemented with 10 µM ROCK inhibitor (Tocris R&D Systems). Re-suspended cells were seeded at a density between 7,500 – 12,500 cells / cm2 on fresh Matrigel-coated plates (Day – 3) ...
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bioRxiv - Neuroscience 2021Quote: ... was dissolved in HEPES-aCSF in the presence of non-mGluR glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; D(-)-2-Amino-5 phosphonopentanoic acid (APV) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in HEPES-aCSF in the presence of non-mGluR glutamate receptor blockers (CNQX, Tocris, 0190, 40 µM; Nimodipine, Tocris, 0600, 1 µM; D(-)-2-Amino-5 phosphonopentanoic acid (APV) ...
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bioRxiv - Cell Biology 2023Quote: ... naïve mGFP+ or YFP+ HFSCs were pretreated with the indicated antagonist (1nM UVI3003; 1uM HX531; 1uM JTE013; 100nM BMS 777607; 1uM AGN 193109) (first four: Tocris Bioscience; last one: R&D Systems) for 30 min prior to corpse or corpse-conditioned media addition ...