2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one citations for Tocris

Quote: ... (15) ESC were grown in N2B27 media supplemented with 2i/LIF (1 mM PD0325901, 3 mM CHIR99021, both from Tocris and 1:500 LIF, made in house). N2B27 media consisted of a 1:1 mix of DMEM/F12 and Neurobasal Medium ...

OGT controls mammalian cell viability by regulating the proteasome/mTOR/mitochondrial axis

Xiang Li, et al., bioRxiv - Cell Biology 2022

Quote: ... Ogt deletion was then induced by the addition of 1 μM 4-hydroxytamoxifen (4-OHT) (TOCRIS, catalog number: 3412). Mouse ESCs were maintained on mitomycin C-treated mouse embryonic fibroblasts (MEFs ...

Inhibition of the melanocortin-3 receptor (MC3R) causes generalized sensitization to anorectic agents

Naima S. Dahir, et al., bioRxiv - Neuroscience 2023

Quote: ... CCK octapeptide (sulfated) (2 and 4 µg/kg, i.p., Tocris) and PYY3-36 were dissolved in a USP Grade sterile phosphate-buffered saline solution (PBS) ...

A PTER-dependent pathway of taurine metabolism linked to energy balance

Wei Wei, et al., bioRxiv - Biochemistry 2024

Quote: ... Exendin-3 (9-39) amide (2081) was purchased from Tocris. Recombinant GDF15 (957-GD ...

Neuroblastoma-associated chromosomal aberrations drive cell identity loss in human neural crest via disruption of developmental regulators

Ingrid M. Saldana-Guerrero, et al., bioRxiv - Cancer Biology 2022

Quote: ... the BMP type-I receptor inhibitor DMH-1 (1 μM, Cat# 4126, Tocris), 10 μM of Rock Inhibitor (Y-27632 ...

Centromere protection requires strict mitotic inactivation of the Bloom syndrome helicase complex

María Fernández-Casañas, et al., bioRxiv - Cell Biology 2024

Quote: ... and Protein Phosphatase 1 inhibitor: Tautomycetin (Tocris Bioscience; 2305, 1 or 5 μΜ).

Regulation of Hippocampal Excitatory Synapses by the Zdhhc5 Palmitoyl Acyl Transferase

Jordan J. Shimell, et al., bioRxiv - Neuroscience 2020

Quote: ... slices were equilibrated for 10 minutes while being continuously superfused at room temperature with oxygenated aCSF at 1-2 ml/min containing picrotoxin (50 μM, Tocris Bioscience, MO, USA) to block GABAA receptor-mediated inhibitory responses.

Cortical lipids containing choline mediate cannabinoid-induced cognitive improvement

Marta Moreno-Rodríguez, et al., bioRxiv - Neuroscience 2024

Quote: ... a third group of animals were treated as those in group 2 with the addition of CB1 receptor antagonist AM251 (1 µM) (Tocris Bioscience, Bristol, UK). After 8 DIV ...

Substrate-specific regulation of mTORC1 activity by G protein-coupled receptors

Samuel J. Atkinson, et al., bioRxiv - Cell Biology 2024

Quote: All cell lines were washed with PBS then starved of amino acids or FBS for 2 hours before treatment with the GPCR antagonists at 1 µM: CNO (Tocris/bio-techne 6329), Isoprenaline/Carvedilol (Sigma/Merck I6504 ...

Defining totipotency using criteria of increasing stringency

Eszter Posfai, et al., bioRxiv - Cell Biology 2020

Quote: ... N2B27 (1-5% KSR) base media was supplemented with 1 µM PD0325901 (Tocris 4192); 3 µM CHIR99021 (Tocris 4423) ...

Sensitisation of colonic nociceptors by IL-13 is dependent on JAK and p38 MAPK activity

Katie H. Barker, et al., bioRxiv - Physiology 2022

Quote: ... pyridone 6 (1 mM; DMSO) and ruxolitinib (1 mM; DMSO) were purchased from Tocris and stock concentrations made up as described ...

BAP1/ASXL complex modulation regulates Epithelial-Mesenchymal Transition during trophoblast differentiation and invasion

Vicente Perez-Garcia, et al., bioRxiv - Developmental Biology 2020

Quote: ... 1 mM SB431542 (Tocris 1614), 0.8 mM VPA (Sigma P4543 ...

Dopaminergic regulation of vestibulo-cerebellar circuits through unipolar brush cells

Jose Ernesto Canton-Josh, et al., bioRxiv - Neuroscience 2022

Quote: ... 1 μM Gabazine (Tocris, 1262), 1 μM NBQX (Tocris ...