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Citations for Tocris :
151 - 200 of 509 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... in 200 μL of mESC maintenance media supplemented with Rho Kinase Inhibitor (Y-27632, 10 μM, Tocris # 1254) (Day -1) ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs at 100% confluence were subject to neural induction using dual SMAD inhibition in N2B27 media (1μM dorsomorphin and 10μM SB431542, both TOCRIS). Day 90 was taken as the final timepoint.
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bioRxiv - Physiology 2024Quote: ... and compared to standard curve prepared by combining serum free H9c2 media with ATP disodium salt (Tocris 3245).
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bioRxiv - Molecular Biology 2021Quote: ... culture medium was replaced by E6 medium supplemented with 6 μM CHIR (Tocris, Bio-Techne, Abingdon, United Kingdom) to initiate differentiation (defined as day 0) ...
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bioRxiv - Neuroscience 2022Quote: ... the culture medium was replaced by PBS and 100 μM of NPEC-caged-serotonin (Cat. No. 3991, Tocris) was added to the dish ...
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bioRxiv - Cell Biology 2021Quote: ... media was replaced with RPMI-1640 supplemented with B27 without insulin and 1 µM XAV 939 (Tocris Biosciences #3748). After an additional 48 hours (Day 5) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The pellets were resuspended in a fresh media supplemented with 10 µM ROCK inhibitor Y-27632 (Tocris Bioscience #1254) for 24 hours to promote the survival of the single cells ...
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bioRxiv - Neuroscience 2024Quote: ... of the cell suspension is reseeded to a new MEF containing 96-well plate pre-loaded with 100µL hPSC media containing 10 µM Rock inhibitor (Y27632, ToCris) and cultured for another 7 days with hPSC media.
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bioRxiv - Genetics 2021Quote: ... which were resuspended in mTeSR Plus media with 10 µM Rho-associated protein kinase inhibitor (ROCKi, Tocris Bioscience #Y-27632) for 24 h ...
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bioRxiv - Cell Biology 2019Quote: Human PSC were grown for 5 days in E8 media(Chen et al., 2011) without FGF-2 and TGF-b but supplemented with 10μM CHIR99021 (Tocris, 4423). Loss of pluripotency was confirmed by RT-qPCR panel of self-renewal ...
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bioRxiv - Neuroscience 2020Quote: ... In this method dual SMAD inhibition was performed using KOSR media supplemented with 10 μM ROCKi and SMAD inhibitors: 5 μM dorsomorphin (Tocris) and 10 μM SB431542 (Tocris ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSc colonies were dissociated and cultured as aggregates in suspension in iPSc media with 10 μM Y27632 (Tocris, Bristol, UK) and 4 ng/ml bFGF (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... Wing discs were then transferred to room temperature media and allowed to recover in the presence of DMSO or 50µM Colcemid (Tocris Bioscience). To block microtubule polymerization wing discs were incubated in 200µM Colchicine at room temperature for 20 minutes before imaging ...
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bioRxiv - Neuroscience 2021Quote: ... Hox propagation was initiated by activation of Wnt signaling using NMP media comprised of E6 medium supplemented with 200 ng/mL FGF8b and 3 μM CHIR99021 (Tocris). This constitutes the “Hox timepoint” of 0hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells embedded in matrigel were incubated at 37C for 15 minutes and covered with PaTOM media supplemented with Y-27632 dihydrochloride (Tocris)
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bioRxiv - Bioengineering 2020Quote: ... Biolamina)-coated 384-well plates in 30 μL of E8 media supplemented with CEPT cocktail or Y-27632 (10 μM, Tocris). After 24 h ...
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bioRxiv - Developmental Biology 2021Quote: ... and then anterior primitive streak was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with A8301 (1 μM; Tocris), LDN193189 (250 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... Neural progenitors were briefly washed (with DMEM/F12) and were then treated with CDM2 basal media supplemented with CHIR99021 (4 μM; Tocris), SB-505124 (2 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with A8301 (1 μM; Tocris), LDN193189 (100 nM ...
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bioRxiv - Neuroscience 2022Quote: ... 150 μL of complete activated media (treated with vehicle (H2O) or 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254)) was very carefully added to each well (for the ROCKi experiments) ...
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bioRxiv - Genomics 2023Quote: ... cells with successful integration were enriched in HEK selection media containing a high concentration (500 nM) of the HMR 1556 (Tocris), a specific IKs blocker with an IC of 10.5 nM.59
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bioRxiv - Bioengineering 2023Quote: ... The media were changed at day 3 to remove the ROCK inhibitor and to add 10 µM DAPT (Tocris Bioscience), which remained until day 7 ...
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bioRxiv - Immunology 2023Quote: Co-culture assays: thymocytes were isolated from untreated C57BL/6 mice and incubated with Nigericin (10 µM, Tocris, 4312) and LPS (1 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... the apical and basolateral chambers were fed PneumaCult ™ ALI media (StemCell #05021) supplemented with 10μM ROCK inhibitor (Tocris Y-27632). Upon reaching confluency ...
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bioRxiv - Genetics 2021Quote: ... 4-6 x 104 cells per well were seeded onto V-bottom 96 well plate in NPC differentiation media suppled with 10 µM Y27632 (Tocris; 1254) to generate embryonic bodies (EBs ...
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bioRxiv - Neuroscience 2022Quote: Cultured aNSCs/NPCs from young and old male C57BL/6 mice were plated onto PDL-coated coverslips in 24-well plates with complete activated media with or without 10 μM ROCK inhibitor (ROCKi) compound Y-27632 (dissolved in H2O) (Tocris, 1254). Forty-eight hours after plating ...
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bioRxiv - Neuroscience 2022Quote: aNSCs/NPCs were cultured in complete activated media until Passage 2-4 and then seeded at a density of 200,000 cells per well in a PDL-coated 12-well plate with complete activated media treated with vehicle (H2O) or 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254). After 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 11320033))-coated coverslips in 24-well plates with complete activated or quiescent media with or without 10 μM Y-27632 (dissolved in H2O) (Tocris, 1254). After 48 hours ...
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bioRxiv - Immunology 2023Quote: ... the apical and basolateral chambers were fed PneumaCultTM ALI media (StemCell #05021) daily supplemented with 10µM ROCK inhibitor (Tocris Y-27632). On Day 8 ...
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bioRxiv - Cancer Biology 2023Quote: Cells grown in normal culture media supplemented with 10 ng/ml doxycycline for 48 hours to induce shRNA or treated with 1 μM TCE5003 (Tocris, 5099) for 22 hours ...
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bioRxiv - Neuroscience 2023Quote: ... A cocktail of drugs was then introduced to the bath application of Ames media including L-AP4 (100 μM, Tocris 0103), CNQX (100 μM ...
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bioRxiv - Developmental Biology 2022Quote: ... the mES cells were seeded on a layer of irradiated ICR mouse embryonic fibroblasts (MEF) and culture in mES medium (see above) supplemented with 2.5 nM PlaB (Tocris, 6070). Cells were passaged every 3-4 days with 0.05% trypsin-EDTA and seeded on MEF feeder layers at split ratios between 1:3 to 1:5 ...
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bioRxiv - Cell Biology 2023Quote: iPSCs were first differentiated into iAT2s and passaged as above in 3D cultures in iAT2 medium (CK+DCI) supplemented for the first 3 days after passaging with 10 uM Y-27632 (Tocris). To generate iAT1s ...
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bioRxiv - Neuroscience 2023Quote: ... Thy1-eGFP cultures were treated with 200 nM and 50 µM by adding NBQX (Cat. No. 1044, Tocris Bioscience, Germany) in the incubation medium for three days ...
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bioRxiv - Cell Biology 2023Quote: ... The indicated drugs were directly added in the culture medium: torin-1 (10 μM) (4247/10, R&D and Tocris), Rotenone (500 nM ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular action potentials were recorded in 3 independent cultures for either genotype in the presence of 50nM nifedipine (Tocris Bioscience) or vehicle using the MaestroPro (Axion Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... the cells in the AggreWell™ plates were cultured in N2/B27 media that was further supplemented with 5 μM Rock-inhibitor (Tocris 1254), 40 μM SB-431542 (Sigma-Aldrich,S4317) ...
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bioRxiv - Cell Biology 2023Quote: ... and distributed to 6-well low-attachment plates containing primitive streak/embryoid body media (10 μM Y-27632 (Tocris/Biotehne, Minneapolis, MN), 3 ng/ml BMP4 (R&D/Biotechne ...
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bioRxiv - Cell Biology 2021Quote: ... B/D medium was supplemented during submerged culture with 1 nM EC23 (light-stable retinoic acid receptor agonist; Tocris, Abingdon, UK). After confluence was reached ...
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bioRxiv - Developmental Biology 2020Quote: ... All cumulus cells were removed from the oocytes by pipetting followed by repeated washings into fresh media using capillary suction and oocytes were placed into HBSS drops containing 1nM of rat kisspeptin-10 (KP-10) (Tocris Bioscience, Bristol, UK). Oocytes were monitored under a fluorescent microscope for 3 min and images were recorded every 30 sec.
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bioRxiv - Molecular Biology 2020Quote: iAT2s plated in ALI culture were pre-treated apically (100 μL) and basolaterally (600 μL) with the indicated concentrations of camostat mesylate (Tocris, #59721-29-5), E-64d (Selleckchem ...
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bioRxiv - Cell Biology 2021Quote: iPSC-airway plated in ALI culture were pre-treated apically (100 μL) and basolaterally (600 μL) with the indicated concentrations of camostat mesylate (Tocris, #59721-29-5), remdesivir (Selleckchem ...
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bioRxiv - Developmental Biology 2021Quote: ... (15) ESC were grown in N2B27 media supplemented with 2i/LIF (1 mM PD0325901, 3 mM CHIR99021, both from Tocris and 1:500 LIF, made in house). N2B27 media consisted of a 1:1 mix of DMEM/F12 and Neurobasal Medium ...
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bioRxiv - Pathology 2020Quote: ... undifferentiated cells were seeded at 1,000 cells/cm2 in mTeSR1 supplemented with 10 μM Y-27632 (Tocris) on Matrigel-coated dishes ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were treated with BAY-826 (TOCRIS) at a final concentration of 1.3 nM for 1-3 days during sprouting.
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bioRxiv - Cancer Biology 2022Quote: ... we treated cells ATN-161 (Tocris, #6058) for 24 hours using concentrations indicated in the figures.
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bioRxiv - Systems Biology 2022Quote: ... cells were treated with Pladienolide B (Tocris) 24 hours prior to irradiation ...
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bioRxiv - Immunology 2023Quote: ... cells were treated with 10μM ML120B (Tocris) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Control cells were treated with UNC2400 (Tocris)— an analog with >1,000 fold lower potency—at the same concentration.
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bioRxiv - Immunology 2023Quote: ... pretreatment of cells with ML120B (Tocris, 10uM)57 was performed for 3h before addition of other reagents ...