Labshake search
Citations for Tocris :
1901 - 1950 of 1953 citations for 7 Bromo 5 methyl 1 2 4 benzotriazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... was used for data shown in Figure 1 according to the manufacturers protocol with the addition of 2.25 mM DFHBI-1T (Tocris #5610) in the reaction mix ...
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bioRxiv - Cell Biology 2020Quote: ... definitive endoderm cells were generated from hESCs (∼90% confluence) by the combination of 1-2µM CHIR-99021 (Tocris, 4423-10MG) [9] and 100ng/ml ActivinA (R&D Systems ...
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bioRxiv - Molecular Biology 2020Quote: ... kin28as strains and their controls were then grown to an OD600 of 0.8 and treated with 6 μM of 1-Naphthyl PP1 (NAPP1; Tocris Bioscience) for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... were recorded from hippocampal GAD67-GFP negative excitatory and GAD67-GFP positive inhibitory neurons incubated in the presence of tetrodotoxin (TTX, 1 μM; Tocris) to block spontaneous action potentials ...
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bioRxiv - Developmental Biology 2021Quote: ... and then anterior primitive streak was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with A8301 (1 μM; Tocris), LDN193189 (250 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with A8301 (1 μM; Tocris), LDN193189 (100 nM ...
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bioRxiv - Developmental Biology 2021Quote: ESCs were then differentiated into definitive ectoderm through the addition of CDM2 basal medium supplemented with A8301 (1 μM; Tocris), LDN193189 (100 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... Mesoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with CDM2 base media supplemented with A8301 (1 μM; Tocris), LDN193189 (250 nM ...
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bioRxiv - Microbiology 2020Quote: ... The concentrations of hydrocortisone for both BEGM and ALI were increased to 0.48 μg/ml and BEGM was further supplemented with the inhibitors 1 μmol/L A83-01 (Tocris, USA), 3 μmol/L isoproterenol (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% bovine serum albumin (Tocris Bioscience) in phosphate buffer solution (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia cells from C57Bl/6 J mice cultured in presence of CSF-1 were treated with Formoterol (10 ng/ml, cat # 1448, Tocris) or vehicle for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Monosynaptic natures of excitatory transmission evoked in the LA- to-aBA pathway was tested by bath application of tetrodotoxin (TTX, 1 μM, Tocris) and subsequently 4-AP (400 μM) ...
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bioRxiv - Cell Biology 2023Quote: ... The indicated drugs were directly added in the culture medium: torin-1 (10 μM) (4247/10, R&D and Tocris), Rotenone (500 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... also known as H-1152 dihydrochloride (CAS 451462-58-1), was Santa Cruz Biotechnology (Dallas, TX). Fasudil (Cat. # 0541/10) and hydroxyfasudil hydrochloride (Cat. #2415) were purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Neuroscience 2023Quote: ... HS-AFM observations of GluA2-γ2 in the desensitized state were performed on mica in buffer A with 0.1% O310F and 1 mM L-quisqualate (Quis, #0188, Tocris, UK). For HS-AFM observations of the GluA2-γ2 and NP1 complex ...
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bioRxiv - Cancer Biology 2024Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... Oxotremorine (10 µM), Riluzole hydrochloride (100 µM), Strychnine (10 µM), Tetrodotoxin (TTX, 1 μM) and ZD7288 (50 µM) were obtained from Tocris. Suramin (100 µM ...
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bioRxiv - Neuroscience 2024Quote: ... were first derived from iPSCs as previously described13: iPSC colonies were grown in N2B27 media supplemented with 1 µM Dorsomorphin (Tocris), 10 µM SB-431542 (Generon) ...
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bioRxiv - Neuroscience 2021Quote: ... To interfere with group I metabotropic glutamate receptor (mGluR) signaling we used bath application of either the mGlu1 receptor antagonist YM298198 (1 μM, Tocris, Germany) or the mGluR5 receptor antagonist MPEP (10 μM ...
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bioRxiv - Neuroscience 2020Quote: ... in either plain Tyrode (stimulated condition) or Ca2+-free Tyrode containing 1mM EGTA and 1 μM TTX (#1069; Tocris Bioscience, Germany) (unstimulated condition) ...
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bioRxiv - Cell Biology 2021Quote: ... B/D medium was supplemented during submerged culture with 1 nM EC23 (light-stable retinoic acid receptor agonist; Tocris, Abingdon, UK). After confluence was reached ...
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bioRxiv - Neuroscience 2020Quote: ... To assess the monosynaptic nature of BLA-EPSCs recordings were obtained in the presence of (in μM): 1 TTX (Tocris Bioscience), 100 4-aminopyridine (4-AP ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Neuroscience 2023Quote: ... Miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) were recorded in the presence of 1 μM Tetrodotoxin (Tocris, 1069) at a holding voltage of -60 mV and +10mV ...
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bioRxiv - Neuroscience 2024Quote: ... To enhance their neuronal fate NSC medium was enriched by 1 µM dorsomorphin (Stemgent, USA) and 10 µM SB-431542 (Tocris, UK). The selected neurorosettes were allowed to form neurospheres in T25 flasks ...
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bioRxiv - Cell Biology 2024Quote: THP-1 cells were differentiated in complete media with phorbol 12-myristate-13-acetate (PMA; 100 nM) (cat no. 1201/1; Tocris, UK) for 72 hr ...
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bioRxiv - Neuroscience 2023Quote: ... Mossy fiber purity of signals was verified at the end of each recording with the application of 1 µM DCG-IV (#0975, Tocris Bioscience). All recordings with a suppression of at least 75% of the signal were used for analysis.
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bioRxiv - Genetics 2023Quote: ... and 10 µg of the Lenti guide puro/blast-gRNA plasmid using 1 mg/ml of PEI transfection reagent (Tocris, 7854). Incubation with the transfection mix was done for 5 hours at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Cells grown in normal culture media supplemented with 10 ng/ml doxycycline for 48 hours to induce shRNA or treated with 1 μM TCE5003 (Tocris, 5099) for 22 hours ...
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bioRxiv - Biophysics 2024Quote: ... in the presence of 1 mM ligands (glutamate and glycine) and in the presence or absence of 30 μM GNE-9278 (Tocris Bioscience).
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bioRxiv - Neuroscience 2019Quote: DIV6–DIV8 mouse cortical neurons either immediately after transfection with HA-Nrxn1α or untransfected were treated for 48 hr (refreshed after 24 hr) with 1 μM tetrodotoxin (TTX) (Tocris, Cat #1069) or 30 μM picrotoxin (PTX ...
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bioRxiv - Neuroscience 2021Quote: ... which is a 1:1 combination of SKSRM and neuronal maintenance medium (NMM) supplemented with 10 μM SB431542 and 1 μM dorsomorphin (both from Tocris Bioscience Inc) for 10 days for neuronal induction ...
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bioRxiv - Biophysics 2022Quote: ... or αCD45 Fab (prepared as in previous work)8 was added in a 1:10 molar ratio to the desired dye - PAJF549 (Tocris Bioscience, UK) or HM-SiR (Sarafluor-650B ...
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bioRxiv - Neuroscience 2019Quote: ... The following compounds were used in pharmacological experiments: 1-Naphthyl acetyl spermine trihydrochloride (“Naspm”, 100 mM in PBS, Tocris Cat No 2766); (+)-MK 801 maleate (“MK-801” ...
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bioRxiv - Neuroscience 2019Quote: ... were measured in voltage clamp at a holding potential of −60 mV in the presence of 1 μM tetrodotoxin (Tocris, Bristol, UK) and 100 μM picrotoxin (Tocris ...
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bioRxiv - Cancer Biology 2019Quote: ... ARL 67156 Trisodium Salt (6-N,N-Diethyl-D-β,γ-dibromomethyleneATP trisodium salt) and POM 1(Sodium metatungstate) were obtained from Tocris Bioscience ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines were maintained in mTeSR1 Plus Medium (Stemcell Technologies 100-0276 and 1 µM of Rock inhibitor (RI) (Tocris 1254). Differentiation was achieved by culturing the cells for 16 days in N2B27 media ...
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bioRxiv - Neuroscience 2024Quote: ... to prevent recruitment of neuronal circuits that could lead to endogenous PACAP1-38 release (Tocris, biotechne; #Tocris 1262/50, #Tocris 0373/50, # Tocris0247/50, #Tocris 1248/50, #Tocris 1069/1).
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding sites were blocked by incubating the cells for 30 min in freshly-prepared blocking solution (1X TTBS, 1% bovine serum albumin (Tocris Bioscience, UK) and 0.25% Triton-X100) ...
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bioRxiv - Pathology 2023Quote: ... CoCl2 (200 μmol/L) and DPD (20 μmol/L) or the HIF-1 inhibitor chetomin (Tocris, Bristol, United Kingdom, 12 nmol/L).
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bioRxiv - Neuroscience 2021Quote: ... were dissolved in magnesium-free HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; LY-367385, Sigma, L4420, 100 μM; MPEP, Tocris, 1212, 10 μM). Type I mGluR agonist DHPG (Tocris ...
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bioRxiv - Neuroscience 2022Quote: ... dissociated hiPSCs were plated at 1×104 cells/cm2 into 6-well plates in the presence of 10 µM ROCK inhibitor (Y-27632, Tocris Bioscience, Bristol, UK). Cortical and hypothalamic differentiation took place on a substrate of geltrex (1:100 ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by incubation with 1μM of IBMX at 37 °C for 15 mins in the presence or absence of the D1R/D5R agonist fenoldopam (1 μM, #1659, Tocris Bioscience, Bristol, UK) or forskolin (5 μM ...
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bioRxiv - Neuroscience 2023Quote: ... The [11C]MeOTf was slowly bubbled into a reaction vial containing 0.2mg 11-(1-Piperazinyl)-5H-dibenzo[b,e][1,4]diazepine precursor (Tocris, Bio-Techne Corp., Minneapolis, MN) and 0.3mL anhydrous MeCN (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... USA), nicotinamide (final concentration, 10 mmol/L) (MilliporeSigma, St. Louis, MO, USA), prostaglandin E2 (final concentration, 1 μmol/L) (Tocris Bioscience, Bristol, UK) B27 supplement (final concentration ...
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bioRxiv - Neuroscience 2023Quote: ... were dissolved in HEPES-aCSF in the presence of non-NMDA glutamate receptor blockers (CNQX, Tocris, 0190, 40 µM; Nimodipine, Tocris, 0600, 1 µM; LY-367385, Sigma, L4420, 100 µM; MPEP, Tocris, 1212, 10 µM). Type I mGluR agonist DHPG (Tocris ...
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bioRxiv - Neuroscience 2024Quote: ... hiPSCs were plated in 6-well plates in Basal Neural Maintenance media (BNMM) (Suppl. Table 1) supplemented with 10 μM SB-431542 (Tocris, Bristol, United Kingdom) and 0.5 μM LDN-193189 (Tocris ...
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bioRxiv - Neuroscience 2021Quote: ... was dissolved in HEPES-aCSF in the presence of non-mGluR glutamate receptor blockers (CNQX, Tocris, 0190, 40 μM; Nimodipine, Tocris, 0600, 1 μM; D(-)-2-Amino-5 phosphonopentanoic acid (APV) ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 1 µM SB431542 (S4317, MilliporeSigma, Burlington, MA, USA) to inhibit TGFβR1 activity or 3µM SIS3 (5291, Tocris/R&D Systems, Minneapolis, MN, USA) to inhibit SMAD3 solubilized in 50 mM DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μM forskolin (solvent: DMSO, 063-02193, Fujifilm Wako Pure Chemicals, Osaka, Japan) and 1 μM LDN193189 (solvent: DMSO, 6053-10, Tocris Bioscience Bristol United Kingdom), with an induction period of 10 days.