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Citations for Tocris :
1201 - 1250 of 1272 citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... definitive endoderm cells were generated from hESCs (∼90% confluence) by the combination of 1-2µM CHIR-99021 (Tocris, 4423-10MG) [9] and 100ng/ml ActivinA (R&D Systems ...
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bioRxiv - Synthetic Biology 2020Quote: Cells expressing stably integrated or transiently transfected rTetR-effectors were assayed by flow cytometry during and after 5 days of 1 μg/mL doxycycline (Tocris) treatment ...
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bioRxiv - Physiology 2021Quote: ... CHO-K1 cells were transfected with cDNAs encoding for hFGF23-V5 and PCs in the ratio of 5:1 and 24 hours later pretreated for 5 hours with Dec-RVKR-CMK (50 μM; Tocris) or D6R (20 μM ...
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bioRxiv - Molecular Biology 2020Quote: ... kin28as strains and their controls were then grown to an OD600 of 0.8 and treated with 6 μM of 1-Naphthyl PP1 (NAPP1; Tocris Bioscience) for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... were recorded from hippocampal GAD67-GFP negative excitatory and GAD67-GFP positive inhibitory neurons incubated in the presence of tetrodotoxin (TTX, 1 μM; Tocris) to block spontaneous action potentials ...
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bioRxiv - Developmental Biology 2021Quote: ... and then anterior primitive streak was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with A8301 (1 μM; Tocris), LDN193189 (250 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... and then definitive ectoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with A8301 (1 μM; Tocris), LDN193189 (100 nM ...
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bioRxiv - Developmental Biology 2021Quote: ESCs were then differentiated into definitive ectoderm through the addition of CDM2 basal medium supplemented with A8301 (1 μM; Tocris), LDN193189 (100 nM ...
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bioRxiv - Developmental Biology 2021Quote: ... Mesoderm was briefly washed (with DMEM/F12) and then treated with CDM2 basal media supplemented with CDM2 base media supplemented with A8301 (1 μM; Tocris), LDN193189 (250 nM ...
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bioRxiv - Microbiology 2020Quote: ... The concentrations of hydrocortisone for both BEGM and ALI were increased to 0.48 μg/ml and BEGM was further supplemented with the inhibitors 1 μmol/L A83-01 (Tocris, USA), 3 μmol/L isoproterenol (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% bovine serum albumin (Tocris Bioscience) in phosphate buffer solution (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: Primary microglia cells from C57Bl/6 J mice cultured in presence of CSF-1 were treated with Formoterol (10 ng/ml, cat # 1448, Tocris) or vehicle for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Monosynaptic natures of excitatory transmission evoked in the LA- to-aBA pathway was tested by bath application of tetrodotoxin (TTX, 1 μM, Tocris) and subsequently 4-AP (400 μM) ...
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bioRxiv - Cell Biology 2022Quote: The mitogen-activated protein kinase kinase 1/2 (MEK1/2) inhibitor U0126 and the p38 MAPK inhibitor SB203580 were purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Neuroscience 2023Quote: ... 1 MgCl2, and 2 CaCl2 (300-310 mOsm, pH adjusted to 7.4 with NaOH) supplemented by SC79 (5uM, Tocris 4635) or A769662 (1uM ...
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bioRxiv - Cell Biology 2023Quote: ... The indicated drugs were directly added in the culture medium: torin-1 (10 μM) (4247/10, R&D and Tocris), Rotenone (500 nM ...
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bioRxiv - Cancer Biology 2023Quote: ... also known as H-1152 dihydrochloride (CAS 451462-58-1), was Santa Cruz Biotechnology (Dallas, TX). Fasudil (Cat. # 0541/10) and hydroxyfasudil hydrochloride (Cat. #2415) were purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Neuroscience 2023Quote: ... HS-AFM observations of GluA2-γ2 in the desensitized state were performed on mica in buffer A with 0.1% O310F and 1 mM L-quisqualate (Quis, #0188, Tocris, UK). For HS-AFM observations of the GluA2-γ2 and NP1 complex ...
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bioRxiv - Neuroscience 2024Quote: ... Oxotremorine (10 µM), Riluzole hydrochloride (100 µM), Strychnine (10 µM), Tetrodotoxin (TTX, 1 μM) and ZD7288 (50 µM) were obtained from Tocris. Suramin (100 µM ...
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bioRxiv - Neuroscience 2024Quote: ... were first derived from iPSCs as previously described13: iPSC colonies were grown in N2B27 media supplemented with 1 µM Dorsomorphin (Tocris), 10 µM SB-431542 (Generon) ...
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bioRxiv - Cancer Biology 2024Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... slices from both hippocampi were washed two times with cold External solution and incubated on ice for 30 min with 1 mg/ml Sulfo-NHS-LC-biotin (Pierce and Tocris) in External solution and slowly rocked ...
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bioRxiv - Neuroscience 2024Quote: ... The synaptic blockers NBQX (#1044) and APV (#0106/1), and the sodium channels blocker tetrodotoxin (TTX, #1078) were obtained from Tocris, Bristol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human total eIF2α Antibody (#AF3997-SP) and human TIMP-1 antibody (#AF970-SP) were purchased from Tocris (Biotechne, United Kingdom). PNGaseF (V4831 ...
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bioRxiv - Biochemistry 2024Quote: ... A549 RIG-I−/− ACE2+ cells (80-90% confluent in 6-well plates) were transduced by adding 10 µg ml−1 Polybrene (Tocris) and recombinant lentivirus to a multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were infected overnight with 1/10 of viral concentrate in media containing 8ug/mL polybrene (Tocris, 7711/10). 24-48h later ...
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bioRxiv - Cell Biology 2024Quote: ... coated 6-well plates with 1-1.5 million cells per well in E8 with 10 μM ROCK inhibitor Y-27632 (Tocris 1254). At day 1 of differentiation medium was exchanged to induction medium (IM ...
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bioRxiv - Neuroscience 2021Quote: ... glutamate and GABA receptor blockers (10 μM NBQX, Tocris, UK, 1044; 5 μM CPP, Tocris, 0247; 5 μM SR-95531, Tocris, 1262; 1 μM CGP-55845, Tocris, 1248) were included in the recording solutions.
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bioRxiv - Neuroscience 2021Quote: ... To interfere with group I metabotropic glutamate receptor (mGluR) signaling we used bath application of either the mGlu1 receptor antagonist YM298198 (1 μM, Tocris, Germany) or the mGluR5 receptor antagonist MPEP (10 μM ...
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bioRxiv - Neuroscience 2020Quote: ... in either plain Tyrode (stimulated condition) or Ca2+-free Tyrode containing 1mM EGTA and 1 μM TTX (#1069; Tocris Bioscience, Germany) (unstimulated condition) ...
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bioRxiv - Neuroscience 2020Quote: ... To assess the monosynaptic nature of BLA-EPSCs recordings were obtained in the presence of (in μM): 1 TTX (Tocris Bioscience), 100 4-aminopyridine (4-AP ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa-fluorophore conjugated secondary antibodies were added in blocking solution for 1 hour at 4°C followed by treatment with Methoxy-X04 (Tocris, 4920) for 15 minutes at room temperature to label amyloid-beta plaques ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Neuroscience 2023Quote: ... Miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) were recorded in the presence of 1 μM Tetrodotoxin (Tocris, 1069) at a holding voltage of -60 mV and +10mV ...
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bioRxiv - Cell Biology 2024Quote: ... + 10 μM Tranylcypromine (2-PCPA) HCl (DMSO; Selleckchem S4246) + 20 μM Forskolin (DMSO; Selleckchem S2449) + 1 μM Ch 55 (DMSO; Tocris 2020) + 5 μM EPZ004777 (DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... To enhance their neuronal fate NSC medium was enriched by 1 µM dorsomorphin (Stemgent, USA) and 10 µM SB-431542 (Tocris, UK). The selected neurorosettes were allowed to form neurospheres in T25 flasks ...
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bioRxiv - Cell Biology 2024Quote: THP-1 cells were differentiated in complete media with phorbol 12-myristate-13-acetate (PMA; 100 nM) (cat no. 1201/1; Tocris, UK) for 72 hr ...
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bioRxiv - Neuroscience 2023Quote: ... maneuvered adjacent to the capillary under study and pressurized injections (200–300 ms, 7 ± 1 PSI) of 100 nM T-peptide (Tocris, USA) with tetramethylrhodamine isothiocyanate (TRITC ...
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bioRxiv - Neuroscience 2023Quote: ... Mossy fiber purity of signals was verified at the end of each recording with the application of 1 µM DCG-IV (#0975, Tocris Bioscience). All recordings with a suppression of at least 75% of the signal were used for analysis.
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bioRxiv - Genetics 2023Quote: ... and 10 µg of the Lenti guide puro/blast-gRNA plasmid using 1 mg/ml of PEI transfection reagent (Tocris, 7854). Incubation with the transfection mix was done for 5 hours at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting after treatment of the cells with 1 µM dTAG-7 (Tocris, 6912) for 3 h ...
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bioRxiv - Cancer Biology 2023Quote: Cells grown in normal culture media supplemented with 10 ng/ml doxycycline for 48 hours to induce shRNA or treated with 1 μM TCE5003 (Tocris, 5099) for 22 hours ...
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bioRxiv - Physiology 2023Quote: ... the cell medium was replaced with the experimental medium (2 ml/well) consisting of DMEM containing 1 nM of 1,25-dihydroxyvitamin D3 (Tocris; Cat. #2551), as described previously58 ...
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bioRxiv - Neuroscience 2024Quote: ... GCaMP6 expression by CreERT2 recombination was induced by the administration of 1 mg of Z-4-hydroxy-tamoxifen (4-OHT, Tocris #3412) (0.2 mL ...
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bioRxiv - Biophysics 2024Quote: ... in the presence of 1 mM ligands (glutamate and glycine) and in the presence or absence of 30 μM GNE-9278 (Tocris Bioscience).
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bioRxiv - Neuroscience 2024Quote: ... animals were intraperitoneally (i.p.) injected with saline (Vehicle, 0.9 % NaCl) or Clonazepine N-Oxide (1 mg/kg) (CNO, Tocris Bioscience Cat# 6329). After i.p ...
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bioRxiv - Biochemistry 2024Quote: ... pelleted cells were loaded with 2 μM Fura-2/AM and either 1 or 5 μM ci-IP3/PM (Tocris #6210) on 15-mm glass coverslips for an hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... which is a 1:1 combination of SKSRM and neuronal maintenance medium (NMM) supplemented with 10 μM SB431542 and 1 μM dorsomorphin (both from Tocris Bioscience Inc) for 10 days for neuronal induction ...
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bioRxiv - Cell Biology 2021Quote: ... Calcium-like peptide 2 (CALP2) and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) were obtained from Tocris (Minneapolis, MN). CaM kinase II and calcineurin inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... VP3.15 was resuspended in 100 mg/mL in DMSO diluted 1:50 in a solution of 5 % Tocrisolve (Tocris®, Minneapolis, USA) to a maximal final volume of 250 µl and the treatment was during 21 days ...