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Citations for Tocris :
1151 - 1200 of 1261 citations for 3 Acetyl 5 4 chlorophenyl 2 methylfuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... ACSF of the same composition as the recording ACSF but with higher concentration (4 mM) of MgCl2 and CaCl2 and MNI-caged glutamate (200 µM; Tocris Bioscience, UK). UV laser pulses (355 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... a combined medium was prepared by collecting 1mL of medium from the wells and mixing with same volume of fresh RPMI+B27 without insulin medium and supplemented with 5 µM IWP2 (Tocris). The medium was changed to 2mL RPMI+B27 without insulin at d5 ...
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bioRxiv - Neuroscience 2020Quote: ... In this method dual SMAD inhibition was performed using KOSR media supplemented with 10 μM ROCKi and SMAD inhibitors: 5 μM dorsomorphin (Tocris) and 10 μM SB431542 (Tocris ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alzet minipumps (model 1004) delivering either the high-affinity 5-HT2B antagonist SB204741 (Tocris Biosciences; 1 mg kg-1d-1) or vehicle (50% dimethyl sulfoxide [Sigma-Aldrich] and polyethylene glycol-400 [Fisher Chemical] ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with 1x PBS for three times and incubated with either 5 µM of Methoxy X04 (Tocris Bioscience) for 20 min at RT or 300 nM of DAPI for 10 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: Larvae were raised on small-batch Drosophila cornmeal dextrose medium (126) prepared with 5 μM L685,458 (Tocris Bioscience, Minneapolis, MN) and processed as above ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mL/kg) or CNO (3.0 mg/kg i.p. in 95% saline, 5% DMSO, 1 mL/kg) (Tocris, Bristol, UK) in counterbalanced order ...
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bioRxiv - Synthetic Biology 2020Quote: Cells expressing stably integrated or transiently transfected rTetR-effectors were assayed by flow cytometry during and after 5 days of 1 μg/mL doxycycline (Tocris) treatment ...
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bioRxiv - Developmental Biology 2020Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.
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bioRxiv - Neuroscience 2021Quote: ... 10 μM NBQX (2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline) and 50 μM AP-5 (Tocris Bioscience, UK) were applied in the bath to block excitatory synaptic responses.
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bioRxiv - Cell Biology 2022Quote: Taxol microtubules were prepared as described above, however were labeled with 16% biotin and 5% Alexa555 tubulin and stored in BRB80T (BRB80, 10 μM Taxol (Tocris)) ...
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bioRxiv - Cell Biology 2023Quote: ... (Optopharma Ltd.) and 20 nM for 1-Oleoyl lysophosphatidic acid sodium salt (LPA) (5 mM stock dissolved in water) (Tocris). As controls ...
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bioRxiv - Biophysics 2023Quote: ... The Piezo1 channel activator Yoda1 (5, 10, 20 or 30 μM, dissolved in 10 mM DMSOt, Tocris Bioscience, Bristol, UK) and the Transient Receptor Potential Vanilloid (TRPV4 ...
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bioRxiv - Neuroscience 2024Quote: ... D1-like and D2-like receptors were blocked by applying SFK 83566 hydrobromide for 5 minutes in the bath at 10 µM (1586, Tocris) and sulpiride at 10 µM (S8010 ...
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bioRxiv - Bioengineering 2024Quote: ... using a stepwise protocol (Fig. S1a): induction from hESCs to primitive streak by treatment with 5 μM CHIR99021 (#4423, Tocris); induction from primitive streak to paraxial mesoderm by treatments with 5 μM CHIR99021 ...
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bioRxiv - Cell Biology 2024Quote: ... The drugs used were 10 µM para-Nitroblebbistatin (motorPharma, #DR-N-111) or 5 µM Y-27632 (Tocris Bioscience, #1254). As a control we used dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2024Quote: RUES2-GLR hESCs grown routinely in NutriStem® hPSC XF were first plated as single cells at a density of 5.2-6.7 x 103 cells/cm2 with 5 µM Y-27632 (Tocris Biosciences, 1254) for the first 24h after plating ...
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bioRxiv - Neuroscience 2021Quote: Bilateral mosaic tadpoles expressing GCaMP6s at stage 48 were immobilized in 2 mM pancuronium dibromide (Cedarlane/Tocris 0693) and embedded in 1% low melting point agarose in a petri dish filled with 0.1X MBSH ...
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bioRxiv - Neuroscience 2021Quote: ... Eye movements were prevented by injection of a-bungarotoxin (1 nL of 2 mg/ml; Tocris, Cat: 2133) into the ocular muscles behind the eye.
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bioRxiv - Cell Biology 2020Quote: ... cells were treated with 50 nM VU0364739 inhibitor (N-[2-[1-(3-Fluorophenyl)-4-oxo-1,3,8-triazaspiro[4.5]dec-8-yl]ethyl]-2-naphthalenecarboxamide) (Lavieri et al., 2010) for 16 h (Tocris Bioscience). For lysosomal inhibition ...
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bioRxiv - Neuroscience 2021Quote: ... Eye movements were prevented by injection of α-bungarotoxin (1 nL of 2 mg/ml; Tocris, Cat: 2133) into the ocular muscles behind the eye.
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bioRxiv - Neuroscience 2020Quote: ... Eye movements were prevented by injection of α-bungarotoxin (1 nL of 2 mg/ml; Tocris, Cat: 2133) into the ocular muscles behind the eye.
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bioRxiv - Neuroscience 2021Quote: ... the same routine was repeated in the presence of the glutamate transport blocker TFB-TBOA 2 μM (Tocris). For both traces the fluorescence change was calculated from the average of the baseline fluorescence intensities for each ROI as ΔF/F = (FSBFI - FSBFI(baseline)/FSBFI(baseline) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were treated with either DMSO or 1 µM of LRRK2 kinase inhibitor (MLi-2, cat.no. 5756, TOCRIS) for 90 mins and then examined for localization ...
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bioRxiv - Neuroscience 2022Quote: Rats were bilaterally infused with either the selective α4β2 nicotinic antagonist DHβE (low dose: 15μg/.5μL/side, high dose 30μg/.5μL/side; Tocris Bioscience, Bristol, UK) the non-selective dopamine receptor antagonist Flupenthixol (15μg/.5μl/side ...
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bioRxiv - Biophysics 2022Quote: ... the 2-methylphenylureaphenylacetyl (MUPA) moiety with LDVPAAK peptide (MUPA-LDVPAAK; now commercially available, Tocris, 7020, supplementary Fig. 12) was custom synthesized by Bio-Synthesis and the amine group of its lysine side chain was reacted with the NHS group of sulfo-SMCC ...
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bioRxiv - Cell Biology 2022Quote: ... LPS was washed off and the macrophages were treated with 2 μM MK6-83 (cat no. 5547, Tocris), 20 μM MLSA-1 (cat ...
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bioRxiv - Genomics 2023Quote: ... and seeded at a seeding density of 10,000–25,000 cells/cm2 in mTeSR supplemented with 2 μM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Neuroscience 2023Quote: ... Candidate ASM order information was as follows: 1-Ethyl-2-benzimidazolinone (Alternative name 1-EBIO, Tocris, CAS: 1041), AA43279 (Focus biomolecules ...
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bioRxiv - Neuroscience 2023Quote: All recordings were done in ACSF-2 without or with 10 μM DQP 1105 (Tocris Bio-Techne, France), 10 μM DQP 1105 + 1μM Ro 25-6981 (Tocris Bio-Techne ...
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bioRxiv - Neuroscience 2023Quote: ... GRP was applied to the spinal cord to evoke Ca2+ oscillations and 2-APB (100 µM, Tocris 1224) 10 min later ...
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bioRxiv - Biophysics 2023Quote: ... whereas IS-2 sample was made up to 100 µM CTZ and 100 µM GYKI- 52466 (Tocris, 1454) prior to centrifugation (75,000 g ...
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bioRxiv - Neuroscience 2021Quote: ... and MNI-caged-L-glutamate ((S)-α-amino-2,3-dihydro-4-methoxy-7-nitro-δ-oxo-1H-indole-1-pentanoic acid) were purchased from Tocris (Bristol, UK). Rhod-2 tripotassium salt was purchased from AAT Bioquest (Sunnyvale ...
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bioRxiv - Immunology 2020Quote: ... anti-IgM/CpG-activated or non-activated B cells were incubated with or without reserpine (5 μM, Tocris, catalogue number: 2747), an inhibitor of VMAT-1 & VMAT-2 for 45min ...
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bioRxiv - Developmental Biology 2022Quote: ... are seeded in a 24-well plate (TPP, #92024) at 30-40k cells per well in E8 + ROCKi (5 µM Y-27632, Tocris #1254). All differentiation media are based on CDM that consists of 5 mg/ml bovine serum albumin (Europa Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... hPSCs were dissociated with EDTA for 10 minutes at 37°C and allowed to aggregate into spheroids of 10,000 cells each in V-bottom 96 well microplates (S-Bio) in E8 medium with ROCK inhibitor (Y-27632, 10 μM) and WNT inhibitor (XAV939, 5 μM, Tocris 3748). The next day (day 0) ...
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bioRxiv - Bioengineering 2023Quote: ... Wnt signaling was inhibited (via the prevention of palmitylation of Wnt proteins by Porcn) from Days 12-19 using the small molecule Wnt-C59 (5 nM; Tocris 5148). After 35 days of differentiation ...
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bioRxiv - Neuroscience 2024Quote: 30 days old mDA neurons were thawed and spun (400 g, 5 min, RT) in a basal medium (Table S2) supplemented with a ROCK inhibitor (Tocris #1254). The cell pellets were then reconstituted in a differentiation medium (Table S2 ...
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bioRxiv - Neuroscience 2024Quote: Cryopreserved 30 days old neurons were thawed in a water bath and centrifuged (400g, 5 min, RT) in basal medium (Table S2) supplemented with ROCK inhibitor (Tocris, #1254). Cell pellets were resuspended in differentiation medium (Table S2 ...
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bioRxiv - Bioengineering 2024Quote: ... seeded in a 6-well plate at a cell density of 0.3 x 106 cells/well and cultured using mTESRTM Plus medium supplemented with 5 µM Y27632 Rho-kinase (ROCK) inhibitor (Tocris; 1254) for the first 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and nor-Binaltorphimine dihydrochloride (norBNI, KOR antagonist, 10 mg/kg, i.p.) (CAS# 113158-34-2) were obtained from Tocris and dissolved in 0.9% sterile saline ...
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bioRxiv - Cell Biology 2022Quote: To observe chromosome alignment in fixed-cell experiments – with the advantage of taking high resolution images and easily selecting many cells for further analysis - cells were released from thymidine block for 7 hours before being treated for 2 hours with RO-3306 (10μM, Tocris) – to synchronise cells at the G2/M boundary - or with a 3-hour treatment with STLC (10μM ...
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bioRxiv - Biophysics 2022Quote: ... 100 μM 2-APB blocking stretch-activated inositol triphosphate receptors (InsP3Rs) on the ER/nuclear membranes (Tocris Bioscience #1224), 100 μM CK-666 (Tocris Bioscience #3950 ...
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bioRxiv - Neuroscience 2020Quote: ... were performed on mice with no knee injections 20 min before and after C21 (2 mg/kg diluted in sterile saline from a stock of 100 mM in ethanol, i.p., Tocris) injection.
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bioRxiv - Neuroscience 2021Quote: ... The mGluR5 specific negative allosteric modulator MPEP hydrochloride (2-Methyl-6-(phenylethynyl)pyridine hydrochloride) was purchased from Tocris (1212) and administered to brain slices at 30 μM by bath perfusion ...
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bioRxiv - Cell Biology 2021Quote: ... organoids cultured for 8 days with airway cell culture medium were followed by inclusion of CHIR99021 (2 µM, Tocris) for additional 10 days with or without DAPT (20 µM) ...
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bioRxiv - Cell Biology 2023Quote: ... 3- (4,5-dimmethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to estimate cell number following the manufacturer’s description (Tocris, # 5224), and absorbance was measured at 550 nm in an EnsightTM 96-well plate reader (Perkin Elmer).
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: ... cells were dissociated from the 100 mm dishes and 2.8-3M cells per well were seeded on the prepared 2-well dishes in N2B27 medium with 10ng/ml bFGF + 10µM Y-27632 ROCK inhibitor (Tocris) media for 3h ...
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bioRxiv - Neuroscience 2024Quote: ... Eye movements were further prevented by injection of α-bungarotoxin (1 nL of 2 mg/ml; Tocris, Cat: 2133) into the ocular muscles behind the eye.