Ethyl 5 1 3 dioxolan 2 yl 2 thiophenecarboxylate citations for Tocris

Quote: ... a combined medium was prepared by collecting 1mL of medium from the wells and mixing with same volume of fresh RPMI+B27 without insulin medium and supplemented with 5 µM IWP2 (Tocris). The medium was changed to 2mL RPMI+B27 without insulin at d5 ...

Application of Airy beam Light sheet microscopy to examine early neurodevelopmental structures in 3D hiPSC-derived human cortical spheroids

Dwaipayan Adhya, et al., bioRxiv - Neuroscience 2020

Quote: ... In this method dual SMAD inhibition was performed using KOSR media supplemented with 10 μM ROCKi and SMAD inhibitors: 5 μM dorsomorphin (Tocris) and 10 μM SB431542 (Tocris ...

Inhibition of Mul1-mediated ubiquitination promotes mitochondria-associated translation

Yuan Gao, et al., bioRxiv - Cell Biology 2021

Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...

Distinctive alteration of presynaptic proteins in the outer molecular layer of the dentate gyrus in Alzheimer’s disease

Hazal Haytural, et al., bioRxiv - Neuroscience 2020

Quote: ... Sections were washed with 1x PBS for three times and incubated with either 5 µM of Methoxy X04 (Tocris Bioscience) for 20 min at RT or 300 nM of DAPI for 10 minutes at RT ...

The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals

Margaret E. Torrence, et al., bioRxiv - Cell Biology 2021

Quote: ... flow cytometer, and the fractions of stained cells were quantified using FloJo 10.6, with Staurosporine (4 h, 5 μM) (Tocris, 1285) used as a positive control for cell death and to help establish gating of the sorted cells.

γ-secretase promotes postsynaptic maturation through the cleavage of a Wnt receptor

Lucas Restrepo, et al., bioRxiv - Neuroscience 2020

Quote: Larvae were raised on small-batch Drosophila cornmeal dextrose medium (126) prepared with 5 μM L685,458 (Tocris Bioscience, Minneapolis, MN) and processed as above ...

Maternal vgll4a promotes blastoderm cohesion enabling yap1-mediated mechano-transduction during zebrafish epiboly

Carlos Camacho-Macorra, et al., bioRxiv - Developmental Biology 2020

Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.

Minhee Analysis Package: An Integrated Software Package for Detection and Management of Spontaneous Synaptic Events

Yong Gyu Kim, Jae Jin Shin, Sang Jeong Kim, bioRxiv - Neuroscience 2021

Quote: ... 10 μM NBQX (2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline) and 50 μM AP-5 (Tocris Bioscience, UK) were applied in the bath to block excitatory synaptic responses.

Piezo1 stretch-activated channel activity differs between bone marrow-derived and cardiac tissue-resident macrophages

A Simon-Chica, et al., bioRxiv - Biophysics 2023

Quote: ... The Piezo1 channel activator Yoda1 (5, 10, 20 or 30 μM, dissolved in 10 mM DMSOt, Tocris Bioscience, Bristol, UK) and the Transient Receptor Potential Vanilloid (TRPV4 ...

CLASP2 facilitates dynamic actin filament organization along the microtubule lattice

NC Rodgers, et al., bioRxiv - Cell Biology 2022

Quote: Taxol microtubules were prepared as described above, however were labeled with 16% biotin and 5% Alexa555 tubulin and stored in BRB80T (BRB80, 10 μM Taxol (Tocris)) ...

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