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Citations for Tocris :
851 - 900 of 906 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2024Quote: The 5-HT1A antagonist N-[2-[4-(2-Methoxyphenyl)-1-piperazinyl]ethyl]-N-2-pyridinylcyclohexanecarboxamide maleate (WAY100635; Tocris Bioscience) at a dose of 1.0 mg/kg.
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bioRxiv - Developmental Biology 2024Quote: ... Monolayers followed the same timing but were cultured in 6-well plates in 3.5 ml of medium and 5 µM XAV939 (Tocris) was included between day 3 and 6.
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bioRxiv - Cell Biology 2024Quote: ... the porcine/human EPSCs were resuspended and seeded in pEPSCs supplemented with 5 μm Y27632 (Tocris, cat. no. 1254). The addition of 5% FBS (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1% Pen/Strep) +2iLIF (10 ng/ml leukemia inhibitory factor + 3 μM CHIR99021 + 1 μM PD0325901 from Tocris, Cat. # 4423 and 4192, respectively). The cells were grown on 0.1% gelatin-coated dishes and usually seeded at a density of 0.5–1.5 × 104/cm2 ...
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bioRxiv - Developmental Biology 2021Quote: ... a combined medium was prepared by collecting 1mL of medium from the wells and mixing with same volume of fresh RPMI+B27 without insulin medium and supplemented with 5 µM IWP2 (Tocris). The medium was changed to 2mL RPMI+B27 without insulin at d5 ...
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bioRxiv - Neuroscience 2021Quote: ... Four of the rats were also infused with 2 μL of either physiological saline or 5 μg/μL yohimbine (Tocris) with a flow rate of 0.5 μL/min as previously reported (Caetano et al. ...
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bioRxiv - Neuroscience 2020Quote: ... In this method dual SMAD inhibition was performed using KOSR media supplemented with 10 μM ROCKi and SMAD inhibitors: 5 μM dorsomorphin (Tocris) and 10 μM SB431542 (Tocris ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alzet minipumps (model 1004) delivering either the high-affinity 5-HT2B antagonist SB204741 (Tocris Biosciences; 1 mg kg-1d-1) or vehicle (50% dimethyl sulfoxide [Sigma-Aldrich] and polyethylene glycol-400 [Fisher Chemical] ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs WT and GPS2-KO cells were treated in the presence or absence of homoharringtonine (HHT) (5 μM, Tocris Bioscience) for 10 min to prevent new translation initiation ...
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bioRxiv - Neuroscience 2020Quote: ... QX-314 (Cat No. 1014); and D-2-amino-5-phosphonovalerate (D-AP5; Cat No. 0106) were purchased from Tocris.
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with 1x PBS for three times and incubated with either 5 µM of Methoxy X04 (Tocris Bioscience) for 20 min at RT or 300 nM of DAPI for 10 minutes at RT ...
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bioRxiv - Cell Biology 2021Quote: ... flow cytometer, and the fractions of stained cells were quantified using FloJo 10.6, with Staurosporine (4 h, 5 μM) (Tocris, 1285) used as a positive control for cell death and to help establish gating of the sorted cells.
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 6 hrs (Figure 6 – figure supplement 2) or 24 hrs after transfection (the remaining experiments) neurons were treated with tetrodotoxin (TTX, 5 µM, Tocris) for ∼16 hours ...
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bioRxiv - Neuroscience 2020Quote: Larvae were raised on small-batch Drosophila cornmeal dextrose medium (126) prepared with 5 μM L685,458 (Tocris Bioscience, Minneapolis, MN) and processed as above ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mL/kg) or CNO (3.0 mg/kg i.p. in 95% saline, 5% DMSO, 1 mL/kg) (Tocris, Bristol, UK) in counterbalanced order ...
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bioRxiv - Synthetic Biology 2020Quote: Cells expressing stably integrated or transiently transfected rTetR-effectors were assayed by flow cytometry during and after 5 days of 1 μg/mL doxycycline (Tocris) treatment ...
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bioRxiv - Developmental Biology 2020Quote: Embryos were incubated at 28°C from the one cell stage to 75% epiboly (8 hpf) in E3 medium containing 5 μM of the Yap1 antagonist verteporfin (5305, Tocris) dissolved in DMSO or the same volume of DMSO as control.
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bioRxiv - Neuroscience 2021Quote: ... 10 μM NBQX (2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline) and 50 μM AP-5 (Tocris Bioscience, UK) were applied in the bath to block excitatory synaptic responses.
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bioRxiv - Biophysics 2023Quote: ... The Piezo1 channel activator Yoda1 (5, 10, 20 or 30 μM, dissolved in 10 mM DMSOt, Tocris Bioscience, Bristol, UK) and the Transient Receptor Potential Vanilloid (TRPV4 ...
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bioRxiv - Cell Biology 2022Quote: Taxol microtubules were prepared as described above, however were labeled with 16% biotin and 5% Alexa555 tubulin and stored in BRB80T (BRB80, 10 μM Taxol (Tocris)) ...
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bioRxiv - Bioengineering 2024Quote: ... using a stepwise protocol (Fig. S1a): induction from hESCs to primitive streak by treatment with 5 μM CHIR99021 (#4423, Tocris); induction from primitive streak to paraxial mesoderm by treatments with 5 μM CHIR99021 ...
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bioRxiv - Neuroscience 2024Quote: ... D1-like and D2-like receptors were blocked by applying SFK 83566 hydrobromide for 5 minutes in the bath at 10 µM (1586, Tocris) and sulpiride at 10 µM (S8010 ...
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bioRxiv - Cell Biology 2024Quote: ... The drugs used were 10 µM para-Nitroblebbistatin (motorPharma, #DR-N-111) or 5 µM Y-27632 (Tocris Bioscience, #1254). As a control we used dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2024Quote: RUES2-GLR hESCs grown routinely in NutriStem® hPSC XF were first plated as single cells at a density of 5.2-6.7 x 103 cells/cm2 with 5 µM Y-27632 (Tocris Biosciences, 1254) for the first 24h after plating ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a fourth group (DZP) received 2 mg/kg diazepam (CAS #439-14-5; Tocris, 2805; German-Ponciano et al., 2020) as a pharmacological control of anxiolytic activity ...
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bioRxiv - Physiology 2021Quote: ... Picrotoxin (100 µM) was used to block GABAA-receptor-mediated currents and D-2-amino-5-phosphonovalerate (APV; 20 µMm, Tocris Bioscience), and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX ...
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bioRxiv - Immunology 2020Quote: ... anti-IgM/CpG-activated or non-activated B cells were incubated with or without reserpine (5 μM, Tocris, catalogue number: 2747), an inhibitor of VMAT-1 & VMAT-2 for 45min ...
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bioRxiv - Neuroscience 2022Quote: ... Intervet AB, Sweden), PCP (phencyclidine hydrochloride, 5 mg/kg, Lipomed AG, Switzerland) or amphetamine (d-amphetamine hydrochloride, 4 mg/kg, Tocris, UK) and recorded for another 60-120 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Both control and CACNA1A+/− networks at DIV49 were treated acutely with 100 µM D-2-amino-5-phosphonovalerate (D-AP5; Tocris, #0106) for 60 min ...
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bioRxiv - Developmental Biology 2022Quote: ... are seeded in a 24-well plate (TPP, #92024) at 30-40k cells per well in E8 + ROCKi (5 µM Y-27632, Tocris #1254). All differentiation media are based on CDM that consists of 5 mg/ml bovine serum albumin (Europa Biosciences ...
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bioRxiv - Developmental Biology 2023Quote: ... hPSCs were dissociated with EDTA for 10 minutes at 37°C and allowed to aggregate into spheroids of 10,000 cells each in V-bottom 96 well microplates (S-Bio) in E8 medium with ROCK inhibitor (Y-27632, 10 μM) and WNT inhibitor (XAV939, 5 μM, Tocris 3748). The next day (day 0) ...
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bioRxiv - Bioengineering 2023Quote: ... Wnt signaling was inhibited (via the prevention of palmitylation of Wnt proteins by Porcn) from Days 12-19 using the small molecule Wnt-C59 (5 nM; Tocris 5148). After 35 days of differentiation ...
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bioRxiv - Neuroscience 2024Quote: 30 days old mDA neurons were thawed and spun (400 g, 5 min, RT) in a basal medium (Table S2) supplemented with a ROCK inhibitor (Tocris #1254). The cell pellets were then reconstituted in a differentiation medium (Table S2 ...
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bioRxiv - Neuroscience 2024Quote: Cryopreserved 30 days old neurons were thawed in a water bath and centrifuged (400g, 5 min, RT) in basal medium (Table S2) supplemented with ROCK inhibitor (Tocris, #1254). Cell pellets were resuspended in differentiation medium (Table S2 ...
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bioRxiv - Bioengineering 2024Quote: ... seeded in a 6-well plate at a cell density of 0.3 x 106 cells/well and cultured using mTESRTM Plus medium supplemented with 5 µM Y27632 Rho-kinase (ROCK) inhibitor (Tocris; 1254) for the first 24 hours ...
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bioRxiv - Biochemistry 2024Quote: ... pelleted cells were loaded with 2 μM Fura-2/AM and either 1 or 5 μM ci-IP3/PM (Tocris #6210) on 15-mm glass coverslips for an hour at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... (15) ESC were grown in N2B27 media supplemented with 2i/LIF (1 mM PD0325901, 3 mM CHIR99021, both from Tocris and 1:500 LIF, made in house). N2B27 media consisted of a 1:1 mix of DMEM/F12 and Neurobasal Medium ...
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bioRxiv - Cell Biology 2021Quote: ... Calcium-like peptide 2 (CALP2) and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) were obtained from Tocris (Minneapolis, MN). CaM kinase II and calcineurin inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... VP3.15 was resuspended in 100 mg/mL in DMSO diluted 1:50 in a solution of 5 % Tocrisolve (Tocris®, Minneapolis, USA) to a maximal final volume of 250 µl and the treatment was during 21 days ...
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bioRxiv - Physiology 2020Quote: ... The MOR antagonist CTAP (D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2, lidocaine, the Ampakine CX614, and the 5-HT4A receptor agonist BIMU8 were obtained from Tocris (ON, Canada).
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bioRxiv - Developmental Biology 2022Quote: ... Cells were centrifuged at 400 rpm for 5 mins and resuspended in NBFAK medium supplemented with 10 μM Rho-associated kinase inhibitor Y-27632 (ROCKi, Tocris Bioscience, UK). Cells were counted and seeded at 20 000 cells per well (or as described in the text ...
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bioRxiv - Neuroscience 2023Quote: ... Drug treatments correspond to a single dose of cocaine hydrochloride at 20 mg/kg (Medisca, cat# 53-21-4, Canada) or D-amphetamine sulfate at 5 mg/kg (Tocris, 2813, UK) at doses known to increase locomotion87 ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was replaced with HBSS containing 6-N,N-Diethyl-β-γ-dibromomethylene-D-adenosine-5′-triphosphate (ARL 67156; Tocris, 1283) at a concentration of 100 μM ...
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bioRxiv - Neuroscience 2022Quote: ... experiments were performed in the presence of GABAA and GABAB blockade (5 µM SR-95531 and 10 µM CGP-52432 respectively; Tocris, Bristol, UK).
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bioRxiv - Neuroscience 2024Quote: ... Cells in dissociation reagent were incubated for 15-20 min at 37οC to ensure dissociation of cell clusters before adding 5 mL of DMEM/F12 containing 0.04% BSA and 10% ROCK inhibitor Y-27632 (1/1000; Tocris; Cat. No. 1254), followed by resuspension by gentle pipetting ...
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bioRxiv - Cell Biology 2023Quote: ... the cells in the AggreWell™ plates were cultured in N2/B27 media that was further supplemented with 5 μM Rock-inhibitor (Tocris 1254), 40 μM SB-431542 (Sigma-Aldrich,S4317) ...
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bioRxiv - Neuroscience 2024Quote: ... and control mice (littermates lacking Cre and/or GqDREADD genes) were given an IP injection of CNO (3 mg/kg, Tocris, Cat. No. 6329, 0.5 mg/ml in saline) >30 minutes before the assessment but was otherwise identical to the training days.
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bioRxiv - Neuroscience 2024Quote: ... and control mice (littermates lacking either the Cre or GqDREADD gene) were given an IP injection of CNO (3 mg/kg, Tocris, Cat. No. 6329, 0.5 mg/ml in saline) >30 minutes before the assessment ...
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bioRxiv - Neuroscience 2024Quote: ... and control mice (littermates lacking either the Cre or GqDREADD gene) were given an IP injection of CNO (3 mg/kg, Tocris, Cat. No. 6329, 0.5 mg/ml in saline) >30 minutes before the assessment but was otherwise identical to the previous 3 training days ...
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bioRxiv - Developmental Biology 2021Quote: ... dissociation and seeded at a density of 5× 104 cells per cm2 in mTeSR1 supplemented with 10μM Y-27362 dihydrochloride (Rocki; Tocris Bioscience, cat. no. 1254).