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Citations for Tocris :
801 - 850 of 953 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... then blocked in Tween 0.1% Tris-buffered saline containing 5% bovine serum albumin (Tocris 5217) for at least 1h at room temperature and subsequently incubated overnight at 4°C with primary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... L-(+)-2-amino-4-phosphonobutyric acid (10 µM; L-AP4, 23052-81-5, Tocris, UK) dissolved in Ames’ medium was bath-applied to the retina.
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bioRxiv - Neuroscience 2021Quote: ... and GluN2BGC−/− mice (P40 – P60) were stereotactically injected with 50 nl of 20 mM solution of kainic acid (Tocris / Biotechne) in 0.9 % NaCl to the left CA1 area of the dorsal hippocampus (AP −1.8 mm ...
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bioRxiv - Cancer Biology 2020Quote: ... and initially validated by switching for 24 hours using 20 nM (Z)-4-Hydroxytamoxifen (4-OHT, Tocris, Minneapolis, Minnesota, 3412), and flow cytometry (see below ...
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bioRxiv - Developmental Biology 2020Quote: ... confluent cells were digested by Accutase to single cells and seeded onto Geltrex-coated 12well plate at the dilution of 1:20 – 1:18 in mTeSR1 with 1uM Thiazovivin (Tocris). Next day ...
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bioRxiv - Neuroscience 2022Quote: ... females with regular estrous cycles underwent intrahippocampal injection of either KA (Tocris; 50 nl of 20 mM, n = 9 mice) or control sterile 0.9% saline (n = 6 mice ...
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bioRxiv - Developmental Biology 2021Quote: ... fish were bathed in 200 ml fresh fish water containing 10 or 20 μM DMH1 diluted from a 10 mM DMH1 (Tocris) stock solution in DMSO ...
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bioRxiv - Cancer Biology 2022Quote: ... and plated at 60,000 cells/cm2 in N2B27 medium supplemented with 20 ng/ml of FGF2 (Cat# 233-FB/CF, R&D) and 4 μM of CHIR 99021 (Cat# 4423, Tocris) and 10 μM of Rock Inhibitor (Y-27632 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then suspended in RPMI media containing 1% low-lipid BSA and 100 nM S1P (S9666, sigma) or 100 nM S1P plus 20 μM JET-013 (2392, Tocris). A total of 100 μl cell suspension as prepared above was added to the upper chamber in a transwell (pore size 5 μm ...
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bioRxiv - Microbiology 2023Quote: ... Reactivation was reported by quantifying the numbers of GFP-positive neurons following the addition of 20-40 µM LY294002 (Tocris) or 60 µM forskolin (Tocris).
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bioRxiv - Developmental Biology 2023Quote: ... The average baseline EPSP was established with a minimum of 20 sweeps before adding glutamate receptor antagonists (10 μM NBQX (Tocris) and 10 μM CPP (Tocris) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCTS and PCLS 8 mm in diameter were cultured in BioR plates in the Bioreactor and treated with sorafenib (10 µM – 20 µM) (Tocris), lenvatinib (0.25 µM – 1.0 µM ...
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bioRxiv - Neuroscience 2021Quote: ... an agonist of group II metabotropic glutamate receptors (mGluR2/3) that reduces release probability in mossy fiber terminals (1 μM, Tocris)(Kamiya et al. ...
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bioRxiv - Neuroscience 2021Quote: ... synaptic GABA currents were recorded in perfusing aCSF containing the glutamate receptor antagonist kynurenic acid (3 mM, Tocris, Ellisville, MO). Recorded neurons were initially held at −70 mV for interneurons and −60 mV for GCs and SGCs and the response to 500 milli-second positive and negative current injections were examined to determine active and passive characteristics ...
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bioRxiv - Neuroscience 2020Quote: ... 4-Butoxy-N-[1,4-dihydro-2-(4-methoxyphenyl)-4-oxo-3(2H)-quinazolinyl]benzamide) and antagonist WRW4 (Trp-Arg-Trp-Trp-Trp-Trp-NH2) were purchased from Tocris Ltd ...
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bioRxiv - Neuroscience 2020Quote: ... The GAT1 blocker NO-711 [(1,2,5,6-tetrahydro-1-[2-[[(diphenylmethylene)amino]oxy]ethyl]-3-pyridinecarboxylic acid hydrochloride] and GAT3 blocker SNAP-5114 [1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid] were purchased from Tocris Bioscience (Minneapolis ...
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bioRxiv - Neuroscience 2021Quote: ... Hox propagation was initiated by activation of Wnt signaling using NMP media comprised of E6 medium supplemented with 200 ng/mL FGF8b and 3 μM CHIR99021 (Tocris). This constitutes the “Hox timepoint” of 0hrs ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.3 or 1 mg/kg of the mGlu2/3 receptor agonist LY379268 (Cat No.: 2453, Batch No.: 9B/232416, CAS No.: 191471-52-0, Tocris Bioscience ...
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bioRxiv - Cell Biology 2023Quote: iPSCs were first differentiated into iAT2s and passaged as above in 3D cultures in iAT2 medium (CK+DCI) supplemented for the first 3 days after passaging with 10 uM Y-27632 (Tocris). To generate iAT1s ...
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bioRxiv - Bioengineering 2023Quote: ... The media were changed at day 3 to remove the ROCK inhibitor and to add 10 µM DAPT (Tocris Bioscience), which remained until day 7 ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular action potentials were recorded in 3 independent cultures for either genotype in the presence of 50nM nifedipine (Tocris Bioscience) or vehicle using the MaestroPro (Axion Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 μM 6-Imino-3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide (SR95531; GABAA receptor antagonist, all from Tocris). The resting membrane potential was measured after whole-cell configuration was achieved ...
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bioRxiv - Molecular Biology 2024Quote: ... AID depletion was triggered using 500 mM of Indole-3-Acetic Acid sodium salt (IAA, auxin analog) (Tocris Bioscience 7932) final ...
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bioRxiv - Microbiology 2024Quote: ... SH- SY5Y cells were differentiated into mature neurons by the reduction of the FBS concentration in the culture medium to 3% and the addition of 100 nM retinoic acid (Tocris) for 72h ...
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bioRxiv - Developmental Biology 2024Quote: Incretin mimetic drug treatments were performed by supplementing unmodified Arc-3 medium with Metformin hydrochloride at 10 µM (Tocris, 2864), Exendin-4 at 10 nM (Tocris ...
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bioRxiv - Immunology 2022Quote: ... or a CD73 inhibitor (0–1 μM) (adenosine 5’-(α, β-methylene) diphosphate (AMP-CP; Tocris) plus ATP (100 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked for 1 hour at RT on a shaker in 5% BSA (Tocris, 5217) with 0.1% Tween-20 (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... and N,N,H,-Trimethyl-5-[(tricyclo[3.3.1.13,7]dec-1-ylmethyl)amino]-1-pentanaminiumbromide hydrobromide (IEM-1460, Tocris) were prepared as 10 mM stocks dissolved in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT1A-R agonist 8-OH-DPAT or antagonist NAN-190 (NAN) were from Tocris (UK). Competition was performed at concentration between 10−11 to 10−5 M ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... cells were treated with RPMI enriched with insulin-free B27 and 5 µM IWP2 (Tocris Bioscience) for 48 hours and then the medium was replaced with RPMI enriched with insulin-free B27 ...
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bioRxiv - Neuroscience 2023Quote: ... The following peptides were delivered in 5 µL of sterile saline: GRP (295 ng, Tocris 1789), Substance P (400 ng ...
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bioRxiv - Neuroscience 2024Quote: ... 2-amino-5-phosphonopentanoic acid (AP5) (cat. # 0106) and picrotoxin (cat. # 1128) were purchased from Tocris. Barium chloride (BaCl2-2H2O ...
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bioRxiv - Neuroscience 2024Quote: ... All experiments were performed in the presence of SR95531 (5 mM, Tocris Bio-Techne, Minneapolis, MN) and CGP55845 (2.5 mM Tocris ...
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bioRxiv - Neuroscience 2021Quote: ... DIV12-13 neurons were pre-treated for 20 minutes with 40 ng/ml of the CXCR2 (CXCL1 receptor in neurons) antagonist SB225002 (Tocris Bioscience), prior to the addition of 40 nM recombinant mouse CXCL1 (R&D Systems ...
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bioRxiv - Neuroscience 2022Quote: ... the slice was transferred to the recording chamber and superfused with ACSF containing 2.5 mM CaCl2 and 20 μM bicuculline methiodide (Tocris, Bristol, UK) at a rate of 3 – 4 ml/min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... + 10 μM Tranylcypromine (2-PCPA) HCl (DMSO; Selleckchem S4246) + 20 μM Forskolin (DMSO; Selleckchem S2449) + 1 μM Ch 55 (DMSO; Tocris 2020) + 5 μM EPZ004777 (DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs were passaged 1:10 or 1:20 and treated with 10 μM ROCK inhibitor (Y-27632 dihydrochloride, eg Tocris #1254). For maintenance ...
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bioRxiv - Cell Biology 2020Quote: ... and diabetic NOD mice (14-26 weeks; n=3) were intra-peritoneally injected with 50mg/kg of Chloroquine diphosphate (Tocris #4109). Two hours post injection ...
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bioRxiv - Cell Biology 2021Quote: Receptors were stimulated as follows: human Angiotensin 1 receptor (AT1R) with Angiotensin II (AngII; Tocris 4474-91-3, in measuring buffer), human β2 adrenergic receptor (β2ADR ...
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bioRxiv - Neuroscience 2022Quote: ... 12-well plates at 3-4 × 105 cells/well in mTeSR1 with 10 µM rho-kinase inhibitor (Y-27632; Tocris, 1254). Medium without the inhibitor was replaced daily until the cells reach 80-100% confluency ...
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bioRxiv - Systems Biology 2020Quote: ... On Day 3 we changed the medium to RPMI + 2% B-27 (minus insulin) + 1uM Xav 939 (Tocris Bioscience, cat. 3748) and incubated the cells for 48 hours ...
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bioRxiv - Cell Biology 2020Quote: ... into fibronectin-coated wells of two 96 well plates per biological replicate to assess proliferation on day 0 (D0) and day 3 (D3) in the presence or absence of 10 μM RARβ antagonist LE135 (Tocris Bioscience) using the cell viability MTS assay ...
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bioRxiv - Biochemistry 2022Quote: ... Positive clones were analyzed by Sanger sequencing with primers #3 and #4 (Integrated DNA Technologies, Supplementary Table S5) and degradation upon dTAG-13 (Tocris, 6605) treatment was confirmed by Western Blot by a-Chaf1a antibody (65) ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 µM 6-imino-3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide (SR95531;GABAA receptor antagonist; all from Tocris Bioscience).
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bioRxiv - Physiology 2023Quote: ... PAH was induced by a single subcutaneous injection of SU5416 (SU:3-(3,5-dimethyl-1H-pyrrol-2-ylmethylene)-1,3-dihydroindol-2-one) (Tocris, Bristol, United Kingdom) in 0.5% carboxymethyl cellulose followed by 3-weeks chronic hypoxia (9-10% O2) ...
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bioRxiv - Neuroscience 2023Quote: ... 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl) hydrazino]carbonyl]benzyl]benzene-sulfonamide (TCN 201, 3 μM dissolved in DMSO; Tocris Bioscience), (αR,βS)-α-(4-Hydroxyphenyl)-β-methyl-4-(phenylmethyl)-1-piperidinepropanol maleate (Ro25-6981 ...
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bioRxiv - Neuroscience 2023Quote: ... 5-(4-Bromophenyl)-3-(1,2-dihydro-6-methyl-2-oxo-4-phenyl-3-quinolinyl)-4,5-dihydro-γ-oxo-1H-pyrazole-1-butanoic acid (DQP 1105, 10 μM dissolved in DMSO; Tocris Bioscience), or 6-Cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were either plated on laminin-coated 10 cm plates at 1.2×107 cells per plate for continued differentiation or expanded in motor neuron progenitor MNP medium (1× N2B27 medium supplemented with 3 mM CHIR99021, 2 mM DMH1 (Tocris, #4126), 2 mM SB431542 ...
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bioRxiv - Physiology 2021Quote: ... Hits from the primary screening were chosen based on % change compared to the control: 20% (LOPAC, CLOUD, Tocris, Prestwick, ReFRAME last batch); 40% (ReFRAME first batch ...
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bioRxiv - Pathology 2022Quote: C57BL/6J mice were randomly assigned to receive either vehicle (100% DMSO) or 20 mg/ kg of Ro5-333543 (Tocris-Bioscience, UK) given subcutaneously ...