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Citations for Tocris :
751 - 800 of 824 citations for Human Dachshund Homolog 1 DACH1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The indicated drugs were directly added in the culture medium: torin-1 (10 μM) (4247/10, R&D and Tocris), Rotenone (500 nM ...
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bioRxiv - Developmental Biology 2023Quote: 3-((3-((9-(Diethylamino)-5-oxo-5H-benzo[a]phenoxazin-2- yl)oxy)propyl)(dodecyl)(methyl)ammonio)propane-1-sulfonate (NR12S, Cat No 7509, Tocris) was used to measure membrane fluidity ...
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bioRxiv - Cancer Biology 2023Quote: ... also known as H-1152 dihydrochloride (CAS 451462-58-1), was Santa Cruz Biotechnology (Dallas, TX). Fasudil (Cat. # 0541/10) and hydroxyfasudil hydrochloride (Cat. #2415) were purchased from Tocris Bioscience (Bristol ...
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bioRxiv - Neuroscience 2023Quote: ... and 10 μM 6-Imino-3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide (SR95531; GABAA receptor antagonist, all from Tocris). The resting membrane potential was measured after whole-cell configuration was achieved ...
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 25 µl PBS together with 0.25 µl of 5 mM 3-[[(7-Hydroxy-2-oxo-2H-1-benzopyran-3-yl) carbonyl]amino]-D-alanine hydrocholoride (HADA) (Tocris) dissolved in DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... HS-AFM observations of GluA2-γ2 in the desensitized state were performed on mica in buffer A with 0.1% O310F and 1 mM L-quisqualate (Quis, #0188, Tocris, UK). For HS-AFM observations of the GluA2-γ2 and NP1 complex ...
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bioRxiv - Cancer Biology 2024Quote: Around 1 x 105 cells were seeded in a 6-well plate for the drug treatment with Brequinar (Tocris, 6196) in a dose-dependent manner for 72 h ...
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bioRxiv - Neuroscience 2024Quote: ... Oxotremorine (10 µM), Riluzole hydrochloride (100 µM), Strychnine (10 µM), Tetrodotoxin (TTX, 1 μM) and ZD7288 (50 µM) were obtained from Tocris. Suramin (100 µM ...
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bioRxiv - Neuroscience 2024Quote: ... were first derived from iPSCs as previously described13: iPSC colonies were grown in N2B27 media supplemented with 1 µM Dorsomorphin (Tocris), 10 µM SB-431542 (Generon) ...
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bioRxiv - Neuroscience 2021Quote: ... glutamate and GABA receptor blockers (10 μM NBQX, Tocris, UK, 1044; 5 μM CPP, Tocris, 0247; 5 μM SR-95531, Tocris, 1262; 1 μM CGP-55845, Tocris, 1248) were included in the recording solutions.
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bioRxiv - Neuroscience 2021Quote: ... To interfere with group I metabotropic glutamate receptor (mGluR) signaling we used bath application of either the mGlu1 receptor antagonist YM298198 (1 μM, Tocris, Germany) or the mGluR5 receptor antagonist MPEP (10 μM ...
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bioRxiv - Physiology 2021Quote: ... L-364,373 (R-L3 or 5-(2-Fluorophenyl)-1,3-dihydro-3-(1H-indol-3-ylmethyl)-1-methyl-2H-1,4-benzodiazepin-2-one) (Tocris Cookson; Bristol, UK) and mefenamic (dimethylphenylaminobenzoic ...
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bioRxiv - Neuroscience 2020Quote: ... in either plain Tyrode (stimulated condition) or Ca2+-free Tyrode containing 1mM EGTA and 1 μM TTX (#1069; Tocris Bioscience, Germany) (unstimulated condition) ...
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bioRxiv - Cell Biology 2021Quote: ... B/D medium was supplemented during submerged culture with 1 nM EC23 (light-stable retinoic acid receptor agonist; Tocris, Abingdon, UK). After confluence was reached ...
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bioRxiv - Neuroscience 2020Quote: ... To assess the monosynaptic nature of BLA-EPSCs recordings were obtained in the presence of (in μM): 1 TTX (Tocris Bioscience), 100 4-aminopyridine (4-AP ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa-fluorophore conjugated secondary antibodies were added in blocking solution for 1 hour at 4°C followed by treatment with Methoxy-X04 (Tocris, 4920) for 15 minutes at room temperature to label amyloid-beta plaques ...
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bioRxiv - Genetics 2022Quote: ... cells were seeded in a 96-well plate on a feeder layer at a low density (~ 1 cell per well) in XEN media supplemented with 10 μM ROCK inhibitor Y-27632 (Tocris #1254) until clones appeared (approximately 5 days) ...
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bioRxiv - Neuroscience 2023Quote: ... Miniature excitatory postsynaptic currents (mEPSCs) and miniature inhibitory postsynaptic currents (mIPSCs) were recorded in the presence of 1 μM Tetrodotoxin (Tocris, 1069) at a holding voltage of -60 mV and +10mV ...
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bioRxiv - Neuroscience 2023Quote: ... 5-(4-Bromophenyl)-3-(1,2-dihydro-6-methyl-2-oxo-4-phenyl-3-quinolinyl)-4,5-dihydro-γ-oxo-1H-pyrazole-1-butanoic acid (DQP 1105, 10 μM dissolved in DMSO; Tocris Bioscience), or 6-Cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX ...
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bioRxiv - Neuroscience 2022Quote: ... was inserted into the guide cannula and a Hamilton Syringe was used to administrate 1 μL of 2 mM pyridoxalphosphate-6-azophenyl-2’,4’-disulfonic acid (PPADS, Tocris, 0625), 1.5 μg/μL S961 (Phoenix Pharmaceuticals ...
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bioRxiv - Neuroscience 2023Quote: ... maneuvered adjacent to the capillary under study and pressurized injections (200–300 ms, 7 ± 1 PSI) of 100 nM T-peptide (Tocris, USA) with tetramethylrhodamine isothiocyanate (TRITC ...
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bioRxiv - Neuroscience 2024Quote: ... To enhance their neuronal fate NSC medium was enriched by 1 µM dorsomorphin (Stemgent, USA) and 10 µM SB-431542 (Tocris, UK). The selected neurorosettes were allowed to form neurospheres in T25 flasks ...
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bioRxiv - Cell Biology 2024Quote: THP-1 cells were differentiated in complete media with phorbol 12-myristate-13-acetate (PMA; 100 nM) (cat no. 1201/1; Tocris, UK) for 72 hr ...
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bioRxiv - Physiology 2023Quote: ... the cell medium was replaced with the experimental medium (2 ml/well) consisting of DMEM containing 1 nM of 1,25-dihydroxyvitamin D3 (Tocris; Cat. #2551), as described previously58 ...
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bioRxiv - Neuroscience 2023Quote: ... Mossy fiber purity of signals was verified at the end of each recording with the application of 1 µM DCG-IV (#0975, Tocris Bioscience). All recordings with a suppression of at least 75% of the signal were used for analysis.
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bioRxiv - Neuroscience 2023Quote: ... and 10 µM 6-imino-3-(4-methoxyphenyl)-1(6H)-pyridazinebutanoic acid hydrobromide (SR95531;GABAA receptor antagonist; all from Tocris Bioscience).
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bioRxiv - Genetics 2023Quote: ... and 10 µg of the Lenti guide puro/blast-gRNA plasmid using 1 mg/ml of PEI transfection reagent (Tocris, 7854). Incubation with the transfection mix was done for 5 hours at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells which efficiently depleted WAPL were identified using confocal microscopy and Western blotting after treatment of the cells with 1 µM dTAG-7 (Tocris, 6912) for 3 h ...
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bioRxiv - Cancer Biology 2023Quote: Cells grown in normal culture media supplemented with 10 ng/ml doxycycline for 48 hours to induce shRNA or treated with 1 μM TCE5003 (Tocris, 5099) for 22 hours ...
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bioRxiv - Cell Biology 2024Quote: ... + 10 μM Tranylcypromine (2-PCPA) HCl (DMSO; Selleckchem S4246) + 20 μM Forskolin (DMSO; Selleckchem S2449) + 1 μM Ch 55 (DMSO; Tocris 2020) + 5 μM EPZ004777 (DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... To obtain an alternative blockade of N-type VGCCs we used N-[[4-(1,1-Dimethylethyl)phenyl]methyl-N-methyl-L-leucyl-N-(1,1-dimethylethyl)-O-phenylmethyl)-L-tyrosinamide (pd173212, purchased from TOCRIS, Bristol, UK) that was used at the final concentration of 5 µM ...
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bioRxiv - Neuroscience 2024Quote: ... GCaMP6 expression by CreERT2 recombination was induced by the administration of 1 mg of Z-4-hydroxy-tamoxifen (4-OHT, Tocris #3412) (0.2 mL ...
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bioRxiv - Biophysics 2024Quote: ... in the presence of 1 mM ligands (glutamate and glycine) and in the presence or absence of 30 μM GNE-9278 (Tocris Bioscience).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The P2Y2R antagonist AR-C118925XX (5-[[5-(2,8-Dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide) was obtained from Tocris (Abingdon, UK). Subsequent dilutions of receptor ligand and other reagents were made in Krebs-Ringer Glucose phosphate buffer (KRG ...
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bioRxiv - Neuroscience 2019Quote: DIV6–DIV8 mouse cortical neurons either immediately after transfection with HA-Nrxn1α or untransfected were treated for 48 hr (refreshed after 24 hr) with 1 μM tetrodotoxin (TTX) (Tocris, Cat #1069) or 30 μM picrotoxin (PTX ...
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bioRxiv - Neuroscience 2021Quote: ... which is a 1:1 combination of SKSRM and neuronal maintenance medium (NMM) supplemented with 10 μM SB431542 and 1 μM dorsomorphin (both from Tocris Bioscience Inc) for 10 days for neuronal induction ...
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bioRxiv - Cell Biology 2021Quote: ... Calcium-like peptide 2 (CALP2) and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7) were obtained from Tocris (Minneapolis, MN). CaM kinase II and calcineurin inhibitors ...
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bioRxiv - Neuroscience 2020Quote: ... VP3.15 was resuspended in 100 mg/mL in DMSO diluted 1:50 in a solution of 5 % Tocrisolve (Tocris®, Minneapolis, USA) to a maximal final volume of 250 µl and the treatment was during 21 days ...
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bioRxiv - Neuroscience 2020Quote: ... 4-[(2S)-2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenyl isoquinolinesulfonic acid ester (KN-62; Tocris and HelloBio); D-AP5 (HelloBio) ...
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bioRxiv - Biophysics 2022Quote: ... or αCD45 Fab (prepared as in previous work)8 was added in a 1:10 molar ratio to the desired dye - PAJF549 (Tocris Bioscience, UK) or HM-SiR (Sarafluor-650B ...
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bioRxiv - Neuroscience 2019Quote: ... The following compounds were used in pharmacological experiments: 1-Naphthyl acetyl spermine trihydrochloride (“Naspm”, 100 mM in PBS, Tocris Cat No 2766); (+)-MK 801 maleate (“MK-801” ...
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bioRxiv - Neuroscience 2019Quote: ... were measured in voltage clamp at a holding potential of −60 mV in the presence of 1 μM tetrodotoxin (Tocris, Bristol, UK) and 100 μM picrotoxin (Tocris ...
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bioRxiv - Genomics 2020Quote: ... supplemented with SMAD inhibitors (DMH-1 2 μM Tocris, Cat.: #4423; SB431542-10 μM Tocris, Cat.: #1614 and CHIR99021 3 μM, CHIR Tocris, Cat.: #4423).
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bioRxiv - Cancer Biology 2019Quote: ... ARL 67156 Trisodium Salt (6-N,N-Diethyl-D-β,γ-dibromomethyleneATP trisodium salt) and POM 1(Sodium metatungstate) were obtained from Tocris Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... as well as the P2Y2R antagonist AR-C118925XX (5-[[5-(2,8-Dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide) were obtained from Tocris (Abingdon, UK). Subsequent dilutions of receptor ligand and other reagents were made in Krebs-Ringer Glucose phosphate buffer (KRG ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines were maintained in mTeSR1 Plus Medium (Stemcell Technologies 100-0276 and 1 µM of Rock inhibitor (RI) (Tocris 1254). Differentiation was achieved by culturing the cells for 16 days in N2B27 media ...
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bioRxiv - Microbiology 2024Quote: To investigate the impact of gallein (3’,4’,5’,6’-Tetrahydroxyspiro[isobenzofuran-1(3H),9’- (9H)xanthen]-3-one) (Cat#3090, Tocris, Minneapolis, MN) and/or INH on Mtb growth ...
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bioRxiv - Neuroscience 2024Quote: ... to prevent recruitment of neuronal circuits that could lead to endogenous PACAP1-38 release (Tocris, biotechne; #Tocris 1262/50, #Tocris 0373/50, # Tocris0247/50, #Tocris 1248/50, #Tocris 1069/1).
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bioRxiv - Neuroscience 2024Quote: ... Cells in dissociation reagent were incubated for 15-20 min at 37οC to ensure dissociation of cell clusters before adding 5 mL of DMEM/F12 containing 0.04% BSA and 10% ROCK inhibitor Y-27632 (1/1000; Tocris; Cat. No. 1254), followed by resuspension by gentle pipetting ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding sites were blocked by incubating the cells for 30 min in freshly-prepared blocking solution (1X TTBS, 1% bovine serum albumin (Tocris Bioscience, UK) and 0.25% Triton-X100) ...