1 2 4 4 4 Pentachloropentafluorobutane citations for Tocris

Quote: ... we added 1% biocytin (Tocris) to the internal solution of whole-cell patch clamp recording and recorded the cell for at least 15 min before withdrawing the electrode ...

The exit of naïve pluripotency contains a lipid metabolism-induced checkpoint for genome integrity

Roshni A. de Souza, et al., bioRxiv - Developmental Biology 2024

Quote: ... PD0325901 (1 µM) (Tocris, 4192) and LIF ...

Epigenetic restoration of differentiation competency via reversal of epiblast regionalisation

Magdalena A. Sutcliffe, et al., bioRxiv - Developmental Biology 2024

Quote: ... 1 μM CHRI99021 (Tocris 4423), 2.5 μM IWP2 ...

Noradrenergic Regulation of Two-Armed Bandit Performance

Kyra Swanson, Bruno B. Averbeck, Mark Laubach, bioRxiv - Neuroscience 2021

Quote: ... Four of the rats were also infused with 2 μL of either physiological saline or 5 μg/μL yohimbine (Tocris) with a flow rate of 0.5 μL/min as previously reported (Caetano et al. ...

Comprehensive genomic analysis reveals virulence factors and antibiotic resistance genes in Pantoea agglomerans KM1, a potential opportunistic pathogen

Robin B. Guevarra, et al., bioRxiv - Genomics 2020

Quote: ... The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience, UK). The used concentrations of inhibitors were selected based on recommendations provided by the manufacturers ...

Pathway-specific deregulation of striatal excitatory synapses in LRRK2 mutations

Chuyu Chen, et al., bioRxiv - Neuroscience 2020

Quote: ... and the Mai Tai eHP was tuned to 725nm for photoactivation of recirculated caged MNI-L-glutamate (2 mM, Tocris)57,58 ...

A fluorescent sensor for spatiotemporally resolved endocannabinoid dynamics in vitro and in vivo

Ao Dong, et al., bioRxiv - Neuroscience 2020

Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...

Presynaptic PTPσ organizes neurotransmitter release machinery at excitatory synapses

Kyung Ah Han, et al., bioRxiv - Neuroscience 2020

Quote: ... QX-314 (Cat No. 1014); and D-2-amino-5-phosphonovalerate (D-AP5; Cat No. 0106) were purchased from Tocris.

Lack of sex differences in gonadotropin-releasing hormone (GnRH) neuron potassium currents and excitability

R. Anthony DeFazio, Suzanne M. Moenter, bioRxiv - Neuroscience 2021

Quote: Potassium currents were isolated pharmacologically during whole-cell voltage-clamp recordings by blocking fast sodium and calcium channels as well as ionotropic receptors for GABA and glutamate (2 µM tetrodotoxin (TTX, Tocris), 300 µM NiCl2 ...

Enhanced food motivation in obese mice is controlled by D1R expressing spiny projection neurons in the nucleus accumbens

Bridget A Matikainen-Ankney, et al., bioRxiv - Neuroscience 2022

Quote: ... the AMPA/NMDA receptor agonist kynurenic acid (2 mM) and the GABA-A receptor antagonist picrotoxin (PTX; 100 uM, Tocris) were present ...

A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down Scaling

Chi-Hong Wu, et al., bioRxiv - Neuroscience 2021

Quote: ... 6 hrs (Figure 6 – figure supplement 2) or 24 hrs after transfection (the remaining experiments) neurons were treated with tetrodotoxin (TTX, 5 µM, Tocris) for ∼16 hours ...

Genome-wide screening in human kidney organoids identifies novel aspects of nephrogenesis

Rosemarie Ungricht, et al., bioRxiv - Cell Biology 2021

Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...

Wilms Tumorigenesis in Human Kidney Organoids

Verena Waehle, et al., bioRxiv - Cancer Biology 2021

Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...

Linear summation of metabotropic postsynaptic potentials follows coactivation of neurogliaform interneurons

Attila Ozsvár, et al., bioRxiv - Neuroscience 2020

Quote: ... During BQA experiments the ACSF solution contained the following substances: 10 μM D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5) (Tocris), 10 μM 2,3-Dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide (NBQX ...

Single cell RNA sequencing in isogenic FUS and TARDBP mutant ALS lines reveals early mitochondrial dysfunction as a common pathway in motor neurons

Christoph Schweingruber, et al., bioRxiv - Neuroscience 2023

Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614] ...

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