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Citations for Tocris :
651 - 700 of 702 citations for 2 6 Amino 9H purin 9 yl ethanol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience, UK). The used concentrations of inhibitors were selected based on recommendations provided by the manufacturers ...
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bioRxiv - Neuroscience 2020Quote: ... and the Mai Tai eHP was tuned to 725nm for photoactivation of recirculated caged MNI-L-glutamate (2 mM, Tocris)57,58 ...
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bioRxiv - Neuroscience 2020Quote: ... or by either bath application or using a custom-made perfusion system (for imaging cells on 12-mm coverslips): 2-AG (Tocris), AEA (Cayman) ...
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bioRxiv - Neuroscience 2021Quote: ... mEPSCs were recorded in aCSF supplemented with 10 μM N-[3-[4-(3-Aminopropylamino)butylamino]propyl]-2-naphthalen-1-ylacetamide trihydrochloride (NASPM, Tocris) to selectively block CP-AMPARs ...
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bioRxiv - Neuroscience 2021Quote: Potassium currents were isolated pharmacologically during whole-cell voltage-clamp recordings by blocking fast sodium and calcium channels as well as ionotropic receptors for GABA and glutamate (2 µM tetrodotoxin (TTX, Tocris), 300 µM NiCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... The GAT1 blocker NO-711 [(1,2,5,6-tetrahydro-1-[2-[[(diphenylmethylene)amino]oxy]ethyl]-3-pyridinecarboxylic acid hydrochloride] and GAT3 blocker SNAP-5114 [1-[2-[tris(4-methoxyphenyl)methoxy]ethyl]-(S)-3-piperidinecarboxylic acid] were purchased from Tocris Bioscience (Minneapolis ...
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bioRxiv - Neuroscience 2022Quote: ... the AMPA/NMDA receptor agonist kynurenic acid (2 mM) and the GABA-A receptor antagonist picrotoxin (PTX; 100 uM, Tocris) were present ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazoledihydro-chloride (MPP; cat# 1991) were purchased from Tocris Bioscience ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... 6 hrs (Figure 6 – figure supplement 2) or 24 hrs after transfection (the remaining experiments) neurons were treated with tetrodotoxin (TTX, 5 µM, Tocris) for ∼16 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and seeded at a seeding density of 10000-25000 cells/cm2 in mTeSR supplemented with 2 µM ROCK inhibitor Y27632 (Tocris) onto laminin-521 coated plates ...
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bioRxiv - Cancer Biology 2021Quote: ... and single-cell suspensions were re-plated in mTeSR1 supplemented with 2 µM ROCK inhibitor (Y-27632 Dihydrochloride Tocris #1254). Transgenic WT29 iPSCs were generated by transfecting PiggyBac expression vector and pBase (Villegas et al ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053], 10 µM SB431542 [Tocris, 1614] ...
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bioRxiv - Neuroscience 2023Quote: Adherent human iPSCs were grown to near confluence before the differentiation to heterogenous spinal/hindbrain neurons was initiated by changing the media for two days to media d1-2 (200 nM LDN193189 [Tocris, 6053] ...
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bioRxiv - Biochemistry 2023Quote: ... Washed membranes were incubated in the hypotonic buffer in the presence of 2 mg/ml iodoacetamide and 50 μm SSR149415 (Tocris) for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... and (3-chloro-4-fluoro-N-[(4-([2-(phenylcarbonyl)hydrazino]carbonyl) phenyl)methyl]benzene-sulfonamide) (TCN-201) (Hansen et al., 2012)were obtained from Tocris Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... 1 MgCl2, and 2 CaCl2 (300-310 mOsm, pH adjusted to 7.4 with NaOH) supplemented by SC79 (5uM, Tocris 4635) or A769662 (1uM ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in an ad hoc defined generic medium (Supplementary Table 2) supplemented with Rho-kinase inhibitor (ROCKi; Tocris) and Primocin ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in DMEM-1%BSA and incubated in suspension for 30 min at 37°C in 10% CO2 and 2 μg/ml CT04 (#CT04-A, Cytoskeleton or 10 μM EHT1864 (#3872, Tocris). The vehicle control included the corresponding dilution of DMSO as needed ...
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bioRxiv - Microbiology 2024Quote: ... the cells were hybridized with V-EV71-C1 probe (Biotechne) at 40°C for 2 hours and then the signals were revealed using TSA Vivid 570 kit (Tocris). The slides were then incubated with blocking buffer and incubated with MAB979 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... another group of animals received an ip injection of WIN55,212-2 (0.5 mg/kg) along with CB1 receptor antagonist SR141716A (0.5 mg/kg, ip) (Tocris Bioscience, Bristol, UK). Rats were randomly selected for each group ...
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bioRxiv - Cell Biology 2022Quote: Compounds which were considered a hit after library screening were purchased from the following commercial suppliers: 2-TEDC (Tocris Bioscience), Anethole-trithione (ATT ...
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bioRxiv - Pathology 2022Quote: About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The slow delayed rectifier potassium current (IKs) was isolated as the current sensitive to 2 µM JNJ303 (Tocris, Bristol, UK). 10 µM nifedipine 5 µM E4031 and 1 µM SEA0400 (all from Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and a fourth group (DZP) received 2 mg/kg diazepam (CAS #439-14-5; Tocris, 2805; German-Ponciano et al., 2020) as a pharmacological control of anxiolytic activity ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was then changed to 3N (50:50 DMEM/F12:neurobasal with N2 and B27 supplements) (Shi et al., 2012) without vitamin A with 2 µM DMH1 (Tocris, 4126), 2 µM XAV939 (Cayman Chemical ...
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bioRxiv - Systems Biology 2020Quote: ... On Day 3 we changed the medium to RPMI + 2% B-27 (minus insulin) + 1uM Xav 939 (Tocris Bioscience, cat. 3748) and incubated the cells for 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was then changed to 3N (50:50 DMEM/F12:neurobasal with N2 and B27 supplements) without vitamin A with 2 µM DMH1 (Tocris, 4126), 2 µM XAV939 (Cayman Chemical ...
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bioRxiv - Physiology 2023Quote: ... the cell medium was replaced with the experimental medium (2 ml/well) consisting of DMEM containing 1 nM of 1,25-dihydroxyvitamin D3 (Tocris; Cat. #2551), as described previously58 ...
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bioRxiv - Cell Biology 2024Quote: ... 1.0×105 cells/cm2 for anti-fibrotic condition consisting in FibroGRO 2% FBS supplemented with 75ng/mL of bFGF and 500nM of TGF-β inhibitor (A83-01, Tocris). iPSC-cFbs were maintained for 7 days in culture replacing medium each second day ...
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bioRxiv - Immunology 2024Quote: ... The following inhibitors were added to the cells for 24 hours before harvesting: the NF-κB inhibitor JSH23 (2 µM, Tocris Bioscience), STING inhibitor H151 (5 µM ...
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bioRxiv - Cell Biology 2024Quote: ... + 10 μM Tranylcypromine (2-PCPA) HCl (DMSO; Selleckchem S4246) + 20 μM Forskolin (DMSO; Selleckchem S2449) + 1 μM Ch 55 (DMSO; Tocris 2020) + 5 μM EPZ004777 (DMSO ...
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bioRxiv - Neuroscience 2023Quote: iPSCs were replated at a density of 2 x 104 cells/cm2 in mTeSR medium containing 10 µM Rho kinase inhibitor (Y-27632, Tocris #5849), which was removed after 24 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibitors were resuspended in DMSO and serially diluted in medium to their final working concentration and added on day 0 (Lats1/2 inhibitor Truli40 (CSNpharm, # CSN26140) or the Limk1 inhibitor Damnacanthal41 (Tocris, # 1936)) ...
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bioRxiv - Neuroscience 2022Quote: ... Non-specific binding was measured in the presence of 2 µM of Xanthine Amine Congener (XAC, Tocris Bioscience, Bristol, United Kingdom), and the resulting values were subtracted from those obtained with [3H]DPCPX35 to determine the specific binding and normalized for protein concentration ...
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bioRxiv - Neuroscience 2023Quote: ... 3-Chloro-4-fluoro-N-[4-[[2-(phenylcarbonyl) hydrazino]carbonyl]benzyl]benzene-sulfonamide (TCN 201, 3 μM dissolved in DMSO; Tocris Bioscience), (αR,βS)-α-(4-Hydroxyphenyl)-β-methyl-4-(phenylmethyl)-1-piperidinepropanol maleate (Ro25-6981 ...
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bioRxiv - Cell Biology 2022Quote: ... The FFA2R antagonist CATPB ((S)-3-(2-(3-chlorophenyl)acetamido)-4-(4-(trifluoromethyl)-phenyl) butanoic acid) was from Tocris (Britsol, UK).
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bioRxiv - Neuroscience 2024Quote: ... The chemogenetic manipulation was carried out 30 minutes after the intraperitoneal injection of CNO at a dosage of 2 mg/kg (TOCRIS Bioscience) or after the administration of saline ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were either plated on laminin-coated 10 cm plates at 1.2×107 cells per plate for continued differentiation or expanded in motor neuron progenitor MNP medium (1× N2B27 medium supplemented with 3 mM CHIR99021, 2 mM DMH1 (Tocris, #4126), 2 mM SB431542 ...
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bioRxiv - Molecular Biology 2024Quote: ... U2OS cells were extracted 1x PBS containing 0.2% Triton-X100 supplemented with PARP inhibitor (olaparib, 1 μM, Selleckchem #S1060) and PARG inhibitor (PDD 00017273, 1 μM, Tocris #5952) to stabilize ADP-ribosylated species for 5 min on ice prior to fixation with 3% paraformaldehyde/2% sucrose for 15 min at ambient temperature ...
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bioRxiv - Genetics 2024Quote: ... On Day 3 (after 48 hr) media was replaced with CDM containing 2 μM of the Wnt signaling inhibitor Wnt-C59 (5148, Tocris Bioscience) in CDM ...
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bioRxiv - Bioengineering 2021Quote: ... the medium was switched to N2B27 medium supplemented with 100 ng/mL VEGFA (ProSpec, CYT-116) and 2 μM forskolin (Tocris Bioscience, 1099) for two days ...
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bioRxiv - Neuroscience 2020Quote: ... 4-[(2S)-2-[(5-isoquinolinylsulfonyl)methylamino]-3-oxo-3-(4-phenyl-1-piperazinyl)propyl] phenyl isoquinolinesulfonic acid ester (KN-62; Tocris and HelloBio); D-AP5 (HelloBio) ...
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bioRxiv - Genomics 2020Quote: ... supplemented with SMAD inhibitors (DMH-1 2 μM Tocris, Cat.: #4423; SB431542-10 μM Tocris, Cat.: #1614 and CHIR99021 3 μM, CHIR Tocris, Cat.: #4423).
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized into groups and treated daily (5 days on, 2 days off) with 1mg/kg SB290157 trifluoroacetate (Tocris, 6860/5) intra-peritoneally ...
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bioRxiv - Immunology 2024Quote: ... Bone marrow-derived macrophages were plated at 2×105 cells/mL and treated with PBS or 500 ng/mL PAM3CSK4 (Tocris Bioscience, 4633) for 6 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... Between 1.5 × 106 and 3.0 × 106 cells were added to each well in 2 ml mTeSR medium with 10 μM Rho-kinase inhibitor Y-27632 dihydrochloride (Tocris, UK # 1254). To aggregate the cells into the microwells ...
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bioRxiv - Neuroscience 2020Quote: ... slices were equilibrated for 10 minutes while being continuously superfused at room temperature with oxygenated aCSF at 1-2 ml/min containing picrotoxin (50 μM, Tocris Bioscience, MO, USA) to block GABAA receptor-mediated inhibitory responses.
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bioRxiv - Neuroscience 2024Quote: ... a third group of animals were treated as those in group 2 with the addition of CB1 receptor antagonist AM251 (1 µM) (Tocris Bioscience, Bristol, UK). After 8 DIV ...
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bioRxiv - Bioengineering 2024Quote: ... FAST-STEM hiPSCs grown to 80% confluency were dissociated into single cells using TypLE for 5 minutes and seeded into the Aggrewell at a density of 1.2×105 cells per-well in 1 mL of mTesR Plus with 10 μg/mL ROCK Inhibitor Y-27632 (Tocris Bioscience, Bristol, UK; 1254) and 1% Penicillin-Streptomycin (Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... was obtained through the administration of exponential doses with concentrations of 1 nM to 10 mM at 2 min intervals before the administration of 10 μM scopolamine (Tocris Bioscience Cat#1414/1G). After another equilibrium period ...