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Citations for Polysciences :
1 - 50 of 99 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formaldehyde (16%, methanol free) was purchased from Polysciences (Warrington, PA). Phosphate-buffered saline with (PBS) ...
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bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
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bioRxiv - Cancer Biology 2022Quote: ... Control and 5-FU-miR-129 groups were treated with either 75 μg of PEI-MAX (control) (Polysciences, Warrington, PA, USA) or 75 μg PEI-MAX + 75 μg 5-FU-miR-129 (5-FU-miR-129) ...
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bioRxiv - Cell Biology 2022Quote: ... After 6 h of LPS treatment 16% Formaldehyde (18814-20, Polysciences Inc.) was added to the medium in the dish to a concentration of 1% and incubated (gently mixing ...
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES) with formaldehyde (molecular grade methanol-free 16% formaldehyde, Polysciences Inc.) at a final percentage of 1% for 30 minutes at room temperature in a vertical rotator ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Cell Biology 2020Quote: ... and cell pellets resuspended in 9.37 ml PBS plus 0.63 ml 16% formaldehyde-EM grade (Polysciences, Inc) and incubated at room temperature for 10 min ...
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bioRxiv - Physiology 2022Quote: ... 16 μg AAV-U6-sgRNA-TBG-Cre plasmid and 144 μl of 1 mg/ml Polyetherimide (PEI) (Polysciences) was mixed in 9 ml OptiMEM (Thermo) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Biochemistry 2019Quote: ... cells were fixed for 10 mins at RT with 4% weight:volume FA (Polysciences, Inc) in 1x PBS ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS and mounted in Mowiol (Polysciences). Images were acquired using an Olympus IX81 inverted microscope (Olympus ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Developmental Biology 2019Quote: Flies of both genders were raised as described above and larvae from second instar to pre-pupal stages collected and dissected directly into fresh 4% EM grade paraformaldehyde solution (from a 16% stock. Polysciences) in PBS with 0.3% TritonX-100 then incubated for 25 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids were diluted in DMEM and incubated for 20 min at RT with Polyethylenimine (PEI, 23966, Polysciences) at a ratio 1:3 DNA:PEI ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Developmental Biology 2021Quote: ... After washing 3 times in PBS sections were mounted with Aquapolymount (Polysciences Inc.), and coverslipped (Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Ovaries were either kept whole or dissected further into ovarioles and fixed for 10 mins using 16% methanol-free formaldehyde (Polysciences, Inc., Warrington, PA, USA) diluted to a final concentration of 4% in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated for 2h at RT and then washed in PBS and further incubated for 30 minutes in 1 ml 50 mM ethanolamine (Polysciences). The beads were then washed 3 times in PBS and left in PBS on a cold room rotator.
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bioRxiv - Neuroscience 2022Quote: ... then washed 3 time in 1xPBS before being mounted with Aqua Polymount (Polysciences, 18606-20). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then washed several times before mounting using aquamount (Polysciences Inc., Cat#: 18606). The following antibodies were used ...
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bioRxiv - Developmental Biology 2023Quote: ... Strips were washed 3 times with PBS and mounted on slides using Aquamount (18606, Polysciences) with the flat side of the strips facing the coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... cell nuclei were stained by DAPI for 1 min at RT and tissues were mounted in Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using Zeiss LSM 880 confocal microscope with a Zeiss 20x/0.8 Plan-Apochromat objective and later analyzed using ImageJ.
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bioRxiv - Neuroscience 2019Quote: ... the sections were washed three times in PBS before being mounted in Aqua-Poly Mount (Polysciences). Slides were allowed to dry for at least one hour and then imaged within two days.
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Cancer Biology 2022Quote: ... BM was washed three times in PBS then mounted on glass-bottom dishes using Polymount medium (Polysciences) on both sides of the mesentery.
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 3 times with PBS for 10 minutes and mounted using AquaPolymount (Polysciences, Warrington, USA). Images were obtained on a Confocal and STED FACILITY line microscope (Abberior Instruments ...
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bioRxiv - Cell Biology 2019Quote: ... Embryos were washed three times with PBT and mounted on glass slides using Aquapolymount (Polysciences, Warrington, PA). Imaging was done on a Zeiss (Oberkochen ...
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bioRxiv - Neuroscience 2022Quote: Slices were washed 3 times for 10 minutes in PBS and mounted with Aqua-Poly/Mount (Polysciences). Images were taken using a Zeiss LSM780 confocal microscope and images were processed using ImageJ software ...
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bioRxiv - Biochemistry 2021Quote: ... the samples were incubated for 3 h at RT in the mixture of EtOH and LR White resin (Polysciences, Inc., Warrington, PA, USA) in the following proportions ...
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bioRxiv - Neuroscience 2021Quote: ... washed again three times for 10 min in 0.1 M PBS and coverslipped with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Biochemistry 2019Quote: ... sections were washed again several times with 0.1 % Tween in PBS and mounted with Aqua Polymount (18606, Polysciences). Sections were visualized using a Leica SP8 confocal laser scanning microscope ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were then washed two more times with PBS before mounting in Aqua/Polymount medium (Polysciences, Inc.) on a glass slide.
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bioRxiv - Neuroscience 2021Quote: ... washed again three times for 10 min in 0.1 M PBS and coverslipped with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped with Aqua-Poly/Mount (Polysciences, Inc.) mounting media.
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bioRxiv - Molecular Biology 2023Quote: ... After washing 4 times with PBS the coverslips were mounted using Aqua-Poly/mount (Polysciences, Warrington, PA, USA). Pictures were made using the Zeiss Axio Imager 2 fluorescent microscope at a 40x zoom ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times with DPBS before embedding with Aqua-Poly/Mount (#18606-20, Polysciences Inc., USA) and a cover glass ...
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bioRxiv - Microbiology 2023Quote: ... The samples were washed three times with PBS and mounted on glass slides using Aqua- Poly/Mount (Polysciences, UK). The stained cells were observed using a confocal microscope system (AX ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with FACS buffer and fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Warrington, PA) diluted in FACS buffer (fixation buffer) ...
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bioRxiv - Genomics 2022Quote: ... The size was defined with a linear model by acquiring time of fight (TOF) of standard sized beads (Megabead NIST Traceable Particles (Polysciences): 60 μm (#64200-15) ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.
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bioRxiv - Cell Biology 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Neuroscience 2019Quote: ... Slides were washed again 3 times for 5 min in PBS and then cover-slipped with Aqua-Poly/Mount (Polysciences, Inc). Images were acquired using a Leica TCS SP8 confocal microscope ...
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bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
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bioRxiv - Cell Biology 2021Quote: ... we washed three times with PNT and stored embryos in 50% glycerol until mounted on glass slides using Aquapolymount (Polysciences, Warrington, PA).
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bioRxiv - Pathology 2019Quote: ... Sections were washed with PBS/5% goat serum 3 times for 3 minutes each and fixed with Aqua-Mount (Polysciences, Warrington, PA). Control kidney sections lacked primary antibodies.