Labshake search
Citations for Polysciences :
401 - 450 of 455 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Neuroscience 2023Quote: Yellow-green fluorescent spheres (ex 441 nm, em 486 nm, Fluoresbrite® YG Microspheres 0.20 µm, Polysciences, Cat. # 17151) were mounted in 2% agarose in CUBIC-R+ medium (RI 1.52 ...
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Microplastic consumption induces inflammatory signatures in the colon and prolongs a viral arthritisbioRxiv - Immunology 2021Quote: The MPs comprised Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1.00 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in aqueous suspension without sodium azide ...
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bioRxiv - Genetics 2021Quote: ... Masson’s Trichrome staining was performed on frozen sections using the Masson’s Trichrome stain kit (Cat# 25088-100; Polysciences; Warrington, PA) and the following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Cancer Biology 2022Quote: ... RIKEN BRC) [22] were mixed with 111 µl of 1 mg/ml PEI MAX® (pH7.5) (Cat. No. 24765-1, Polysciences) in Opti-MEM™ I Reduced Serum Medium (Cat ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Genomics 2023Quote: ... Expi293F cells at a density of 3×106 cells/mL were transfected with 1mg/L of culture of plasmid complexed with PEI Max 40K (cat. 24765-100, Polysciences) in a 1:2 plasmid:PEI w/w ratio in 40mL Opti-MEM (cat ...
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bioRxiv - Neuroscience 2023Quote: ... on a microscope slide with 1.6 µL of a solution containing equal measures of polystyrene beads (Polybead CAT No. 00876-15, Polysciences Inc.) and 30 mM muscimol (CAT No ...
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bioRxiv - Genetics 2023Quote: ... and an ITR-flanked AAV plasmid (pAAVsc-JeT-MCOLN1-pA) using polyethyleneimine (PEI) (Cat. no 23966, Polysciences, Warrington PA, USA). AAV was collected from serum-free culture media at 72 h and from the cells and media 120 h post transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293 cells were co- transfected with 3μg of either V2RWT or V2RT360A along with 2μg of βarr1-mYFP in presence or absence of 2μg of Ib30 with help of polyethylenimine (Polysciences; cat. no. 23966) reagent (21μl ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Immunology 2020Quote: ... HEK293A were transfected with equal amounts of the heavy and the corresponding light chain plasmid using linear PEI (Polysciences Cat# 23966). The media was changed after 24 h to BASAL media (50% DMEM 12430 ...
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bioRxiv - Neuroscience 2019Quote: ... for 10 min on 2% agarose pad and then immobilized using 1.5 μl Polystyrene 0.10 micron microspheres and a coverslip (Polysciences, Inc, Cat# 00876). GCaMP3 imaging was performed on a Zeiss AxioImager microscope using a Q-Imaging Rolera EM-C2 EMCCD camera ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Immunology 2021Quote: ... by co-transfecting heavy chain and light chain expressing plasmids using polyethylenimine (PEI, Linear, MV∼25,000, Polysciences, Inc. Cat. No. 23966) and culture in 37.0C degree shaker at 125RPM and 8% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were transfected with indicated receptor constructs (3.5 μg) and βarr2 (βarr2WT/DM) constructs (3.5 μg) using polyethylenimine (PEI) linear (Polysciences, Cat no. 19850) at a ratio of 1 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 100 μg plasmid in complex with 250 μg PEI MAX 4000 (Polysciences, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells were seeded to achieve 50–60% confluence the following day and transfected using PEI (polyethylenimine, Polysciences Inc., Cat# 23966) as follows ...
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bioRxiv - Genomics 2023Quote: ... 15μg of MigR1-OsTIR-GFP vectors were co-transfected with 15μg of pCL-Eco packaging plasmids into HEK293T cells at 90% confluence through PEI (Polysciences Cat#23966). Virus were collected at 48h and 72h respectively and filtered through 0.45μM filters to remove cell debris ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again washed in 1M PBS (4x10 minutes) and were then mounted and coverslipped with coverslips of #1.5 thickness using Aqua-Poly/Mount (Polysciences cat: 18606).
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bioRxiv - Biochemistry 2024Quote: ... The DNA was added to 200 μl of DMEM and mixed with 7.5 μl of PEI reagent (Polysciences Inc cat# 23966). Tubes were incubated at room temperature for 15 min before adding to cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... following by curing the resin overnight at 4°C in a plastic embedding mold (Cat no.-15899-50, Polysciences Europe GmbH, Germany). Polymerized sample blocks were further hardened by overnight baking at 70°C.
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bioRxiv - Immunology 2019Quote: ... the crude nuclei were precipitated by centrifuge then were resuspended and bind with Bio-Mag Plus Concanavalin A coated beads (Polysciences, cat# 86057), after blocking for 5 min ...
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bioRxiv - Neuroscience 2019Quote: ... slices were floated onto Superfrost™ glass slides with the membrane confetti in contact with the slide and Menzel-Gläser #1.5 glass coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 100 µg plasmid in complex with 250 µg PEI MAX 4000 (Polysciences, Inc, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post transfection ...
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bioRxiv - Biochemistry 2022Quote: ... media was changed for DMEM high glucose supplemented with only 2.5% FBS and cells were transfected using PEI (Polysciences; cat. no. 23966) as follow ...
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bioRxiv - Biochemistry 2021Quote: ... Stable cell lines expressing N-terminal Flag tagged receptor constructs in pcDNA3.1 vector were generated by transfecting HEK-293 cells with 7 μg of plasmid DNA using polyethylenimine (PEI) (Polysciences, Cat. no. 19850), followed by selection using G418 (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... slides were dipped in distilled water and Menzel-Gläser #1,5 coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
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bioRxiv - Neuroscience 2019Quote: ... slices were floated onto Superfrost glass slides with the membrane confetti in contact with the slide and Menzel-Gläser #1,5 glass coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
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bioRxiv - Evolutionary Biology 2019Quote: ... Slides were washed once more in PBS in the dark and stored for imaging with Aqua Poly/Mount (Polysciences, Inc. Cat. # 18606). Images were at the UC Irvine Optical Biology Core Facility using a Zeiss LSM700 confocal microscope under 20x objective ...
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bioRxiv - Immunology 2021Quote: ... HEK293A cells were transfected with equal amounts of the heavy and the corresponding light chain plasmid using linear PEI (Polysciences Cat# 23966). The media was changed after 24 h to BASAL media (50% DMEM 12430 ...
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bioRxiv - Biophysics 2022Quote: ... Solutions were then placed into fresh tubes along with a single polystyrene bead (1/8 inch Polyballs, Cat. No. 17175; Polysciences, Warrington, PA) and agitated again for 30 min to remove trace hexadecane still present in solution ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... 100-200 µg of purified bacmid in 1 mL water were incubated with 400 µg of 25000 MW polyethyleneimine (PEI; Polysciences Cat# 23966) in 1 mL water at 55 degC for 30-45 minutes to sterilize ...
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bioRxiv - Biochemistry 2023Quote: HEK293T cells cultured in DMEM with 10% Fetal Calf Serum were transfected using linear polyethylenimine (MW ∼25,000, Cat#23966 Polysciences Inc., Warrington, PA), 40 µg/ 10 cm dish and plasmid DNA ...
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bioRxiv - Biophysics 2020Quote: ... 1 L of cells at 1×106 cells were transfected with complexes composed of 250 μg of plasmid DNA and 750 μg of polyethylenimine (Polysciences, Cat. # 24765-1). For the expression involving kifunensine ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Microbiology 2022Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells using PEI Max (Polysciences, Cat# 24765-1). At 48 h posttransfection ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was produced in HEK293T cells by transfecting plasmid DNA of interest with packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences, Cat# 23966-1). Media were replaced 6-8 hrs following transfection ...
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bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...
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bioRxiv - Molecular Biology 2023Quote: ... melanin of part of samples were breached before oil red O staining using Melanin Bleach Kit (Cat # 24883 Polysciences, Inc., Warrington, PA, USA). After washing in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...