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Citations for Polysciences :
151 - 200 of 604 citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RIKEN BRC) [22] were mixed with 111 µl of 1 mg/ml PEI MAX® (pH7.5) (Cat. No. 24765-1, Polysciences) in Opti-MEM™ I Reduced Serum Medium (Cat ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Immunology 2021Quote: ... using PEI Max (1 mg/mL, Polysciences, Inc.) at a 3:1 ratio (PEI Max:DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... Six□micrograms of PEI-Max (Polysciences, #24765-1) were used for transfection ...
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bioRxiv - Microbiology 2021Quote: ... using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). To measure NS3/4A activity ...
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bioRxiv - Microbiology 2021Quote: ... 1 M anti-digoxygenin coated polystyrene beads (Polysciences) were conjugated to the peptide construct through λ-DNA of the peptide construct by incubation in a buffer containing 10 mM Tris HCl pH 7.5 and 150 mM NaCl for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). For the NKG2D blocking assay ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). OT-1 cells were analyzed by flow cytometry using a FACSCanto II cell analyzer (Becton Dickinson ...
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bioRxiv - Biophysics 2019Quote: ... 1 μM anti-digoxigenin coated polystyrene beads (Polysciences), diluted in a buffer containing 10 mM Tris HCl pH 7.5 and 50 mM NaCl were added to the imaging chamber and incubated for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl PEI-max (1 μg/μl; Polysciences) was mixed with DNA (1 μg/ml cells ...
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bioRxiv - Neuroscience 2023Quote: ... or GluN2B using PEI Max (Polysciences, 24765-1). 24 h after transfection ...
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bioRxiv - Microbiology 2023Quote: ... 500 µL PEI-max (1 µg/µL; Polysciences) was mixed with DNA (1 µg/mL cells ...
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bioRxiv - Immunology 2023Quote: ... 72 uL 1 mg/mL polyethylenamine (Polysciences 23966), and 600 uL DMEM ...
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bioRxiv - Immunology 2023Quote: ... using PEI Max (1 mg/mL, Polysciences, Inc.) at a 3:1 ratio (PEI Max:DNA) ...
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bioRxiv - Microbiology 2023Quote: ... for 1 min and Phosphotungstic acid (Polysciences, 25088D) for 10 mins with washes between each step ...
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bioRxiv - Developmental Biology 2024Quote: ... positioned in an embedding mold (18646A-1, Polysciences) containing 100% OCT ...
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bioRxiv - Bioengineering 2021Quote: ... and packaging plasmids pCMV-dR8.91 (900 ng) and pCMV-VSV-G (100 ng) with 12 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences 24765-1) into HEK293T cells that had been grown to 60-80% confluence in 6-well plates ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Immunology 2020Quote: ... 1 milligram of plasmid DNA encoding HIV-1 Env per 1 liter of cells was diluted in DMEM and mixed with polyethyleneimine (PEI, Polysciences, Inc.). The DNA was allowed to complex with PEI for 25 min and then cells were added ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then transfected with 1 μg of JFH1-QL/GLuc2A in vitro transcribed RNA (1 μg) using polyethylenimine (Polysciences, Inc.). Supernatants were harvested and mixed with Renilla luciferase buffer and substrate ...
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bioRxiv - Microbiology 2024Quote: ... and NiV-M-GFP at a 9:1:5 ratio by using polyethylenimine (PEI) at 1 mg/ml (Polysciences, 23966-100). At 48hrs posttransfection ...
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bioRxiv - Molecular Biology 2021Quote: 100 million cells were seeded in 15 cm dishes and the protein-DNA contacts were cross-linked using 1% (v/v) formaldehyde (Polysciences, 04018-1) for 10 min at room temperature with shaking ...
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bioRxiv - Developmental Biology 2022Quote: Worms were mounted without anesthetic on 10% agarose pads in a 1:1 solution of M9 buffer and 0.1 μM polystyrene beads (Polysciences, Inc., Warrington, PA) essentially as described (Rosu and Cohen-Fix 2020) ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells/mL culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa PEI (Polysciences, 24765-1) per mL culture volume ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected using PEI-max (#24765-1, Polysciences) with 100 ng of pSNL-N2-C or pSNL-N2-Muc7 and 100 ng of pSNL-N1-”C interactors” ...
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bioRxiv - Cell Biology 2020Quote: ... and 20 μl polyethylenimine (PEI; 1 mg/ml) (Polysciences) was added ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1:3 25 kDa linear Polyethylenimine (Polysciences, #23966). The cells were grown for 20-28h ...
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bioRxiv - Cell Biology 2021Quote: ... according to manufacturer instructions or PEI (Polysciences 23966-1). 48 hours post transfection ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Neuroscience 2021Quote: ... molecular weight (MW) 25,000 (Polysciences, cat. no. 23966-1) was used as the transfection reagent ...
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bioRxiv - Neuroscience 2022Quote: ... Polyethylenimine (PEI, Polysciences, Inc., 1 mg/mL, pH 7.0), or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... using 5 μL of Polyethylenimine (PEI) (Polysciences, 23966-1) at 1 mg/mL stock concentration ...
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bioRxiv - Neuroscience 2019Quote: ... The beads are 1 µm silica (Polysciences 24326-15), functionalized through reaction with tri-ethoxy-sliane-PEG biotin (Nanocs #PEG6-0023) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were stained with 1% aqueous uranyl acetate (Polysciences, Inc. ...
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Rationally designed protein bandpass filters for controlling cellular signaling with chemical inputsbioRxiv - Synthetic Biology 2023Quote: ... and 600 ng of polyethyleneimine (24765-1, Polysciences, Inc.). For drug treatment experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with polyethyleneimine (PEI) (Polysciences; #23966-1) with pCMV-dR8_91 and pVSV-G packaging plasmids ...
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bioRxiv - Immunology 2023Quote: ... 1.6 mL of 40 K polyethylenimine (Polysciences 24765-1) (1 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 µl transfection reagent PEI (1 µg/µl, Polysciences) was added and incubated for 30 min at RT and added to the 10 cm dish with HEK293T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... 7.35µg of PEI MAX (Polysciences Inc., Cat. #24765-1) and 10mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... samples were stained with 1% aqueous uranyl acetate (Polysciences, Inc. ...