Labshake search
Citations for Polysciences :
1 - 50 of 639 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and packaging plasmids pCMV-dR8.91 (900 ng) and pCMV-VSV-G (100 ng) with 12 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences 24765-1) into HEK293T cells that had been grown to 60-80% confluence in 6-well plates ...
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bioRxiv - Cell Biology 2021Quote: ... 12 µl PEI transfection reagent (Polysciences, 24765) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... and 12 μg of pLKO-shRNA-puro using 63 μL of polyethylenimine (PEI – 1 μg/μL; Polysciences - 24765) mixed in 750 μL of 10 mM HEPES pH 7.4 and 150 mM NaCl in water ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared mixing 8-12 μg of bacmid DNA in 100 μL 1xPBS and 12 μg polyethyleneimine (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) in 100 μL 1xPBS and the reaction was allowed to proceed for 20-30 minutes before addition to an Sf9 monolayer containing well ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection reactions were prepared mixing 8-12 μg of bacmid DNA in 100 μL 1xPBS and 12 μg polyethyleneimine (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) in 100 μL 1xPBS and the reaction was allowed to proceed for 20-30 minutes before addition to an Sf9 monolayer containing well ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared by the addition of 2-10 ug of bacmid DNA to 100 μl 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine “Max” MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Biochemistry 2021Quote: ... Transfection reactions were prepared by the addition of 2-10 μg of bacmid DNA to 100 μL 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine ‘‘Max’’ MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...
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bioRxiv - Immunology 2023Quote: HEK293T cells were cultured in 12-well plates and transfected by polyethyleneimine (PEI, Polysciences) with following constructs ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... each 6 cm dish of cells was transfected with 2-12 μg of poly(ethylenimine) (Polysciences, Eppelheim, Germany) and 1-6 μg of plasmid DNA ...
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bioRxiv - Biophysics 2022Quote: ... Cells were plated to 1.5 x 105 cells per well in 12-well plates 24 hours prior to transfection with polyethylenimine PEI (Polysciences). The primary transfection mixtures contained 125 ng pFLuc-TAR-140 reporter plasmid ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 12 µL of 100 mg/mL linear polyethyleneimine (PEI) at molecular weight 25 kDa (Polysciences, Warrington, PA, USA) into 38 µL of a 150 mM NaCl solution ...
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bioRxiv - Biophysics 2024Quote: Adherent HEK293T cells were seeded in a 12-wells plate and transfected at 70% confluency using PEI MAX® - Transfection Grade Linear Polyethyleneimine Hydrochloride (MW 40,000) (Polysciences) at a 1:3 DNA ...
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells were transfected at 70% confluency in a 6-well plate or 12-well plate using PEI MAX® - Transfection Grade Linear Polyethyleneimine Hydrochloride (MW 40,000) (Polysciences) at a 1:3 DNA ...
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bioRxiv - Cell Biology 2024Quote: ... was co-transfected with packaging plasmids pMD2.G (500 ng) and psPAX (500 ng) into 293T cells using 12 μl PEI (Polysciences) in a 6-well plate for 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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bioRxiv - Microbiology 2023Quote: HEK293T cells (2×106 cells on a 10 cm dish) were transfected with 12 μg of plasmid DNA and 36 μg of polyethylenimine hydrochloride/PEI MAX (Polysciences, Inc., PA, USA). The cells were then cultured for 20 h ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells (2 × 106 cells on a 10 cm dish) were transfected with 12 μg of plasmid DNA and 36 μg of polyethylenimine hydrochloride (PEI) MAX (Polysciences, Inc., PA, USA) for 20 h ...
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bioRxiv - Neuroscience 2021Quote: ... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
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bioRxiv - Neuroscience 2024Quote: ... Sections stained with antibodies against MBP or pMLC2/Pan-NF were placed in Liberate Antibody Binding (L.A.B. Polysciences Inc.), after the initial PBS wash ...
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bioRxiv - Biophysics 2020Quote: ... and GFP in 1:1:1 ratio using polyethyleneimine (54) (Polysciences, Inc.; 23966-1; lot: 705149). We verified each construct by sequencing before and after subcloning ...
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bioRxiv - Neuroscience 2023Quote: ... at a 1:1:1 ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum free DMEM at 16 hours post transfection ...
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bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
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bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
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bioRxiv - Microbiology 2023Quote: ... and pHelper (CellBio Labs) at a 1:1:1 ratio using polyethyleneimine (PolySciences). Supernatant was collected 72hrs post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... at a 1:1:1 molar ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum-free DMEM at 16 hours post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
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bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
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bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
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bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1% glutaraldehyde (Polysciences) in 0.1 M cacodylate buffer (Polysciences ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences, #23966-1) diluted in 18.8 mL Opti-MEM® ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...