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Citations for Polysciences :
1 - 50 of 576 citations for Recombinant Human Neuropilin 1 Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Neuroscience 2020Quote: IgSF8-Fc and Tenascin-R-Fc proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Immunology 2020Quote: ... or pαH-SARS-CoV-2 RBD-Fc using 2 mg polyethylenimine (Polysciences, Cat# 24765). 6 days after transfection ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... complemented with 10% FCS and 2mM glutamine) were transfected using 4μg polyethylenimine (PEI, Polysciences Inc., Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Immunology 2020Quote: ... at 10,000 cells in 100μl PBS and human serum opsonised Fluoresbrite™ Yellow Green Microspheres 1μm (Polysciences) were added at a concentration of 10:1 for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
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bioRxiv - Immunology 2021Quote: ... Env plasmids were co-transfected in Human Embryonic Kidney 293T or GnT1-293S cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid (23 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant truncated human TSEN complexes were produced in human embryonic kidney (HEK) 293F cells by cotransfection using linear polyethyleneimine (PEI) (MW 25000, Polysciences) and incubated for 72 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Biophysics 2020Quote: ... and GFP in 1:1:1 ratio using polyethyleneimine (54) (Polysciences, Inc.; 23966-1; lot: 705149). We verified each construct by sequencing before and after subcloning ...
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bioRxiv - Immunology 2024Quote: ... Env plasmids were co-transfected with MuLV Gag plasmid and pMV-Rev 0932 in human embryonic kidney 293T (or GnT1-) cells using polyethyleneimine (PEI Max, Polysciences, Inc.). 48h later ...
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bioRxiv - Neuroscience 2023Quote: ... at a 1:1:1 ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum free DMEM at 16 hours post transfection ...
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bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
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bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
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bioRxiv - Microbiology 2023Quote: ... and pHelper (CellBio Labs) at a 1:1:1 ratio using polyethyleneimine (PolySciences). Supernatant was collected 72hrs post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
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bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
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bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
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bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
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bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
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bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
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bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Developmental Biology 2019Quote: Fluorescent red beads of 1 uM diameter (1:500, 18660-5, Polysciences) were injected into the KV of embryos at the 6-somite stage ...
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bioRxiv - Microbiology 2019Quote: ... followed by 1 h of fixation in 1% osmium tetroxide (Polysciences Inc.). After extensive wash with dH20 ...
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bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...