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Citations for Polysciences :
1 - 50 of 65 citations for Recombinant Human Interleukin 7 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Biochemistry 2020Quote: ... HA-tagged constructs and bioGFP-tagged constructs using MaxPEI (Polysciences) in a ratio of 3/1 PEI/DNA ...
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bioRxiv - Immunology 2022Quote: ... pH 7 (Polysciences) to transfect 293Freestyle cells ...
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bioRxiv - Bioengineering 2023Quote: ... Membrane associated receptor candidates were transfected by polyethylenimine (PolySciences # 23966). Cells were seeded on Neuvitro Poly-D-lysine coated sterile German glass coverslips (Fisher Scientific #NC0343705 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were transfected with 8 μ of plasmid DNA (SF-tagged LRRK2 WT, or SF-tagged LRRK2 G2019S) per 10cm2 dish using polyethyleneimine (Polysciences) solution ...
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bioRxiv - Neuroscience 2021Quote: ... 2500 sterilized FITC-tagged microspheres (Polysciences Inc., PA, USA) suspended in a 100µl of PBS were slowly injected into the CCA using a 33G hypodermic needle (TSK Laboratory International ...
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bioRxiv - Immunology 2023Quote: ... or Myc-tagged constructs by using polyethylenimine (PEI, Polysciences, 23966). After 24 hr later ...
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bioRxiv - Neuroscience 2023Quote: ... 80uL of PEIMAX (Polysciences #49553-93-7) were then added ...
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bioRxiv - Bioengineering 2024Quote: ... using PEI MAX (Polysciences, 49553-93-7). Plasmids were acquired through the NIH HIV Reagent Program ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with the Strep-tagged constructs using polyethylenimine (PEI, Polysciences), in a ratio of 3:1 for PEI:DNA ...
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bioRxiv - Genetics 2019Quote: ... then transfected with the corresponding SF-TAP-tagged DNA constructs using PEI reagent (Polysciences) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Microbiology 2021Quote: ... using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 600 ng of PsPax2 (Addgene # 12260) ...
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bioRxiv - Microbiology 2023Quote: ... Biofilm competitions utilized 7 mm polystyrene beads (Polysciences, Inc, Warrington, PA) as a surface for cell attachment ...
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bioRxiv - Bioengineering 2024Quote: ... 174 μl (1 μg/ul) polyethylenimine (PEI, Polysciences, 49553-93-7) was vortex mixed with 1930 μl Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
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bioRxiv - Bioengineering 2023Quote: ... separate samples of 10 kPa solution were made substituted with Nile blue-tagged acrylamide (Polysciences Inc. #25395-100) at 1:1000.
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were transfected with indicated receptor constructs (3.5 μg) and βarr2 (βarr2WT/DM) constructs (3.5 μg) using polyethylenimine (PEI) linear (Polysciences, Cat no. 19850) at a ratio of 1 ...
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bioRxiv - Biophysics 2021Quote: ... Cells in a single dish were transfected with 1.25 μg of c-term EGFP tagged hTMEM175 plasmid using 3.75 μg of PEI 25 k (Polysciences, Inc). Electrophysiological recordings were performed 48-72 hr after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Canada) and transiently co-transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimin (PEI; Polysciences Inc., USA). PEI was dissolved in water as 1 mg/mL stock solution with pH of 6.5–7.5 and kept at −20°C ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: HEK293TR cells were transfected with pcDNA4/TO encoding SNAP-tagged β2AR and A2AR with PEI transfection reagent (Polysciences Europe GmbH) (PEI:DNA ratio of 3:1) ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T were seeded in 10-cm dishes then transfected with 10 ug of myc-tagged hnRNPA1 and hnRNP A1dm mutant using polyethyleneimine (PolySciences). Cells were treated with 100 uM 4-SU 16 hours prior to UV-crosslinking ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were co-transfected with both plasmids containing the Venus fragments-tagged bait and prey ORF (100 ng for each) which were pre-mixed and incubated with polyethylenimine (PEI) (Polysciences Inc.) and OptiMEM (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... by pre-mixing C-terminal AVI-tagged ZM197-ZM233V1V2 or C-terminal AVI-tagged CAP256-wk34c80-RnS-3mut-2G with a furin expression plasmid using Polyethylenimine (PEI) 40K (Polysciences, Inc.) or Turbo293 (Speed BioSystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then grown to optical density of 1.2×106 cells/mL at 37 °C and transfected with Flag-Tagged plasmids using PEI (Polysciences, 23966-2). After 48 h of transfection ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293FT cells were grown at 1 x 106 cells/mL and transfected with C-terminal FLAG-tagged WT or active-site mutants cloned in pcDNA3.1 using PEI-max (Polysciences #24765-100). Cells were treated with 10 mM sodium butyrate for ∼16 h after transfection and cultured for two additional days ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with the corresponding plasmids using polyethylenimine (1 μg/μL [pH 7]; Polysciences) in a 1:3 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... Huh-7 were transfected with linear 25 kDa polyethyleneimine (PEI, Polysciences Inc., Warrington, PA, USA) by using a 3:1 ratio of PEI/DNA (as described in [58]) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Montreal, Canada) and transiently transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimine (Polysciences Inc., Warrington, PA, US) in Cellstar® PS 96-well cell culture plates (Greiner Bio-One ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... The transfection was performed using linear polyethyleneimine hydrochloride (40,000, PEI MAX, Polysciences, Cat# 49553-93-7). For each vector 5× 15cm dishes were transfected and subsequently AAV particles were purified from cell pellet and media using iodixanol gradient purification as previously reported79.
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bioRxiv - Cell Biology 2022Quote: ... A total of 1 µg per one million cells of the plasmids containing CMV promoter and 3×FLAG-tagged geneswere mixed with a three-fold excess (w/w) of polyethylenimine MAX 40 kDa (Polysciences, Warrington, PA, USA) in 4 ml OptiPro (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were transiently transfected with appropriate cDNA using PEI (Polyethylenimine, linear, MW 25000; Polysciences, Inc).
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The day after seeding, pcDNA3L-His-CAMYEL plasmid were transiently transfected into cells using linear polyethyleneimine (PEI, m.w. 25 kDa) (#23966, Polysciences, Warrington, PA, USA). The DNA-PEI complex mixture was added into the cells at the ratio of 1:6 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...