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Citations for Polysciences :
1 - 50 of 262 citations for Recombinant Human Interleukin 2 Receptor Alpha Fc chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... or pαH-SARS-CoV-2 RBD-Fc using 2 mg polyethylenimine (Polysciences, Cat# 24765). 6 days after transfection ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Membrane associated receptor candidates were transfected by polyethylenimine (PolySciences # 23966). Cells were seeded on Neuvitro Poly-D-lysine coated sterile German glass coverslips (Fisher Scientific #NC0343705 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Neuroscience 2020Quote: IgSF8-Fc and Tenascin-R-Fc proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were transfected with indicated receptor constructs (3.5 μg) and βarr2 (βarr2WT/DM) constructs (3.5 μg) using polyethylenimine (PEI) linear (Polysciences, Cat no. 19850) at a ratio of 1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Canada) and transiently co-transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimin (PEI; Polysciences Inc., USA). PEI was dissolved in water as 1 mg/mL stock solution with pH of 6.5–7.5 and kept at −20°C ...
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bioRxiv - Cancer Biology 2020Quote: ... complemented with 10% FCS and 2mM glutamine) were transfected using 4μg polyethylenimine (PEI, Polysciences Inc., Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Montreal, Canada) and transiently transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimine (Polysciences Inc., Warrington, PA, US) in Cellstar® PS 96-well cell culture plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2019Quote: ... and either 2-µm YG microspheres (Polysciences; 17155-2) or 15-µm scarlet microspheres (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 2 ml 2% PFA (Polysciences, Inc., 18814). After 30 min incubation at room temperature (RT) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 0.2ml of 2% PFA + 2% glutaraldehyde (PolySciences) per well and subsequently stained with 0.2ml of crystal violet solution (8% crystal violet + 20% ethanol in ddH2O) ...
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bioRxiv - Cancer Biology 2019Quote: ... in FACS buffer containing either 2-µm YG microspheres (Polysciences; 17155-2) or a known concentration 15-µm scarlet microspheres ...
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bioRxiv - Cancer Biology 2021Quote: ... at a ratio of 2:2:1 using polyethylenimine (Polysciences, Warrington, PA). After 18 hours of transduction ...
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bioRxiv - Cell Biology 2022Quote: ... Polyethylenimine (Polysciences, 23966-2), Opti-MEM™ (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... Addgene)[70] in a ratio of 5:3:2 using polyethylenimine (24765-2, Polysciences). Virus supernatant was harvested 30 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... or PEI (POLYSCIENCES 23966-2) into HEK293T cells grown on a 10cm dish (Moffat et al. ...
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bioRxiv - Synthetic Biology 2023Quote: Polyethylenimine (PEI) (Polysciences 23966-2) was used to transfect HEK293FT cells ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent 2 μm beads (Polysciences Fluoresbrite® YG Microspheres 2.00μm 18338-5 ...
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bioRxiv - Molecular Biology 2019Quote: ... linear mw 25,000 (Polysciences 23966-2). Cells were washed with DMEM or DMEM-F12 2-4 hours after transfection reagent was added (Longo et al. ...
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bioRxiv - Cell Biology 2021Quote: ... After fixation with 2% glutaraledhyde (Polysciences) in PBS (pH7.4) ...
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bioRxiv - Bioengineering 2021Quote: ... polystyrene latex microspheres (Polysciences, 18142-2) with our HELP gels ...
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bioRxiv - Bioengineering 2023Quote: ... 2-sulfoethyl methacrylate (PolySciences, Warminster, PA) was added to a final concentration of 5 mM ...
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bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 mg of PEI “MAX” (Polysciences) (40 kDa ...
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bioRxiv - Genetics 2020Quote: ... and PEI Max (Polysciences, Inc. 24765-2). At 72 hr posttransfection ...
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bioRxiv - Bioengineering 2021Quote: ... 2-aminoethyl methacrylate (AEMA, 3.04 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 2% paraformaldehyde solution (Polysciences, #18814-20 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Bioengineering 2022Quote: ... A concentration of 2 μg mL-1 plasmid DNA and 2 μg mL-1 plasmid DNA linear 25kDa polyethyleneimine (Polysciences) dissolved in OptiPRO serum free medium (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: All larvae were fixed in 2 mL round bottom tubes with 2 mL of fixation solution [1.5% Formaldehyde (Polysciences, Inc.), 0.1 M PIPES (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... A 2 mm region just rostral of the hippocampus was drop-fixed in 2% PFA/2.5% glutaraldehyde (01909-100, Polysciences, Inc.) at 4°C overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with PEI (Polysciences, #23966-2) using 15 μg of CIGAR-seq vector ...
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bioRxiv - Bioengineering 2022Quote: ... 2 µg/ml linear 25 kDa polyethylenimine (Polysciences), and OptiPRO SFM medium (4% final concentration ...