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Citations for Polysciences :
1 - 50 of 678 citations for Rat T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... The surface attached cells were fixed in 4% formaldehyde (Polysciences) for 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... by co-transfection heavy chain and light chain expressing plasmids using polyethylenimine (PEI, Linear, MV∼25,000, Polysciences, Inc. Cat. No. 23966) and culture in 37 °C degree shaker at 125RPM and 8% CO2.
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bioRxiv - Immunology 2021Quote: ... by co-transfecting heavy chain and light chain expressing plasmids using polyethylenimine (PEI, Linear, MV∼25,000, Polysciences, Inc. Cat. No. 23966) and culture in 37.0C degree shaker at 125RPM and 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Immunology 2021Quote: ... Two vectors containing paired TCRα and β chains were transfected together into Phoenix packaging cells using PEI MAX (Polysciences). Supernatant containing retroviruses was used for infection into mouse T cell hybridoma with an NFAT-GFP reporter gene (Matsumoto et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and beta-gal plasmid using PEI Max 40000 (PolySciences, USA). The next day transfection medium was exchanged with fresh medium ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... the glass surfaces were treated with 0.5% glutaraldehyde (Polysciences, Inc.) for 30 min ...
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bioRxiv - Immunology 2021Quote: ... HEK293A cells were transfected with equal amounts of the heavy and the corresponding light chain plasmid using linear PEI (Polysciences Cat# 23966). The media was changed after 24 h to BASAL media (50% DMEM 12430 ...
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bioRxiv - Microbiology 2022Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells using PEI Max (Polysciences, Cat# 24765-1). At 48 h posttransfection ...
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bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Immunology 2022Quote: ... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... the polystyrene beads containing surface primary amino groups (PolySciences, 17145-5, Diameter 3 μm) were incubated with 10 mM of Sulfo-NHS-LC-LC-Biotin (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The surface was visualised by adding black 10 μm polystyrene microspheres (Polysciences, Warrington, USA) on top of the mucus ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Immunology 2022Quote: ... with equal amounts of Ig heavy and light chain expression vectors using polyethylenimine Max (PEI- MAX, Polysciences) as a transfection reagent ...
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bioRxiv - Immunology 2020Quote: ... Both MPs and NPs were surface modified with branched polyethylenimine (Polysciences, MW=70,000, Warrington, PA) through reaction via EDC and sulfo-NHS with a non-toxic level of PEI conjugated to PLGA particles (~6.5 µg PEI/mg PLGA ...
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bioRxiv - Immunology 2022Quote: ... the pCAGGS-RBD plasmid was transfected into ∼5×107 adherent HEK-293/T17 cells (ATCC CRL-11268) in a T-175 using PEI (Polyethylenimine, linear 25,000 m.w.(Polysciences, Warrington, PA, USA). Plasmid was mixed at a 1:3 ratio with PEI (20ug of plasmid ...
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bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
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bioRxiv - Neuroscience 2020Quote: ... tissue samples were placed into embedding moulds (Polysciences, T-8), filled with warm gelatine (20% ...
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bioRxiv - Bioengineering 2023Quote: ... The light and heavy chain expression plasmids of LH03 or LH05 were mixed with 25 kDa linear polyethylenimine (PEI, Polysciences) and transiently transfected in HEK293E cells ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 1 cm catheter with biofilm formed on inside surface was fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Immunology 2020Quote: ... HEK293A were transfected with equal amounts of the heavy and the corresponding light chain plasmid using linear PEI (Polysciences Cat# 23966). The media was changed after 24 h to BASAL media (50% DMEM 12430 ...
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bioRxiv - Microbiology 2020Quote: ... transfections were done in T-75 flasks using polyethylenimine (Polysciences Inc, PA, USA). Transfections were done according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Treated rat explants were fixed in 4% PFA (Polysciences, Inc., Warrington, PA, USA) at room temperature for 40 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the pigmentation of RPE cells was removed using Melanin Bleach Kit (Polysciences), followed by 3 washes with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with benzidine in combination with the Differential Quick Stain Kit (PolySciences, Warrington, PA) (manufacturers protocol) ...
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bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
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bioRxiv - Immunology 2022Quote: Spleens and MLN were harvested from OT-II (Thy1.1) mice and T lymphocytes were enriched using nylon wool fiber columns (Polysciences, Inc., Warrington, PA). CD4+ T cells were positively selected with CD4 (L3T4 ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were infiltrated with propylene oxide for 10 min and embedded using a Poly/Bed 812 kit (Polysciences, Warrington, PA, USA; Cat:08792). The specimens were sectioned at 200 nm using an ultramicrotome (EM-UCT ...
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bioRxiv - Immunology 2020Quote: ... the PolyLink Protein Coupling Kit (Polysciences) was used as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: PolyLink Protein Coupling Kit (Polysciences, Inc) was used to prepare 50µl of fluorescence beads solution ...
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bioRxiv - Biophysics 2019Quote: ... we used a protein coupling kit (Polysciences) to coat micro-spheres with WGA14 ...
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bioRxiv - Cancer Biology 2022Quote: ... Picrosirius Red stain Kit (Polysciences Inc., PA, USA) was used with a slight modification of the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The Fungi-Fluor® Kit (Polysciences, PA, USA) was applied following the manufacturers protocol ...
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bioRxiv - Biophysics 2021Quote: ... via Carbodiimide reaction (PolyLink Protein Coupling Kit, Polysciences Inc.). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... and Picrosirius red staining kit (Polysciences, Inc., Warrington, PA) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transiently transfected at a density of 3 × 106 cells/mL using linear polyethylenimine (Polysciences). 24 h following transfection ...
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bioRxiv - Genomics 2022Quote: ... cells are transfected using PEI (Polysciences) with 1 ug of total DNA comprising 500 ng of piggyBac (fused or unfused ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... cells using polyethylenimine (PEI) (Polysciences, Inc.) to generate the lentiviral particles ...
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bioRxiv - Genetics 2019Quote: ... Samples were embedded using the Poly/Bed 812 Kit (Polysciences). After pure fresh resin embedding and polymerization in a 65°C oven (TD-700 ...
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bioRxiv - Cancer Biology 2021Quote: ... we used Rapid PTAH Stain Kit (Polysciences, Warrington, PA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were stained with Weigert’s Hematoxylin Kit (Polysciences, Warrington, USA) for 8 min and washed for 10 min under running tap water ...