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Citations for Polysciences :
251 - 300 of 301 citations for Rat Heat Shock Protein Beta 2 HSPB2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 spike gene expression vector in conjunction with lentiviral vectors p8.91 and pCSFLW using polyethylenimine (PEI, Polysciences, Warrington, USA). Pseudotype-containing supernatants were harvested 48 hours post-transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted embryos were transferred via a hand drawn glass capillary to a 2 uL drop of M9 buffer containing ∽100 polystyrene beads 20 um in diameter (Polysciences Inc.), sandwiched between two pieces of #1.5 coverglass ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Slides were stained using a Masson Trichrome stain kit (Polysciences, 25088-1) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the pigmentation of RPE cells was removed using Melanin Bleach Kit (Polysciences), followed by 3 washes with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Collagens in the organoids were stained using Masson’s Trichrome Stain Kit (Polysciences) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2023Quote: ... and embedded in epoxy resin (Spurr Low Viscosity Embedding Kit, Polysciences Inc) as described earlier (84) ...
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bioRxiv - Immunology 2023Quote: ... and collagen staining was performed using a Picrosirius Red Stain Kit (Polysciences) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was run twice on a BD Canto II flow-cytometer with 2 μm diameter fluorescent beads (Polysciences, Warminster, PA, USA) as a size and fluorescence standard ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Molecular Biology 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells/mL culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa PEI (Polysciences, 24765-1) per mL culture volume ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Neuroscience 2022Quote: ... mt-GCaMP6f: AAV1) and transfer plasmids were mixed and diluted in saline before mixing with saline-diluted polyethylenimine (PEI, Polysciences #23966-2) and brief vortexing ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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bioRxiv - Microbiology 2020Quote: ... Calculations of relative fluorescence per cell were done by normalizing red chlorophyll fluorescence per cell to 2 μm reference fluorescent beads (catalog no. 18604; Polysciences, Warrington, PA, USA) as previously described [72] ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Cancer Biology 2021Quote: ... tumors were stained using the picrosirius red stain kit (Polysciences Inc., Warrington, PA). For fibronectin staining ...
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bioRxiv - Bioengineering 2021Quote: ... Picrosirius red staining was performed using a commercially available kit (24901, Polysciences, Inc.) at day 3 and day 7 ...
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bioRxiv - Genomics 2021Quote: ... III and undifferentiated collagens using the picrosirius stain kit (Polysciences, Inc., Pennsylvania, USA). The observer was blinded to the sample identification to avoid subconscious bias ...
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bioRxiv - Cancer Biology 2022Quote: ... Collagen staining was performed using a Picrosirius red staining kit (#24901; Polysciences, Inc.) per the manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... stained with benzidine (staining heme) and Differential Quik Stain Kit (PolySciences, Warrington, PA) to differentiate globin and DNA as described before5 ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Cell Biology 2022Quote: ... Bones were then washed for 10 minutes in PBS (3 times) and stained with a 1:1 mixture of 2% aqueous osmium tetroxide (cat# 23310-10, Polysciences, Inc., Warrington, PA, USA) and 5% potassium dichromate for 48 hours ...
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bioRxiv - Immunology 2020Quote: 0.75 μm diameter fluorescent beads (Fluoresbrite YG Carboxylate Kit I 21636-1, Polysciences Inc.) were suspended at a concentration of 0.04% (w/w ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were embedded using the Poly/Bed 812 Embedding Kit (Polysciences, #08792-1) and sectioned to a thickness of 200 to 250 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... sections were rehydrated and stained for collagen using a Picro Sirius red staining kit (PolySciences). After staining ...
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bioRxiv - Cancer Biology 2019Quote: ... Picrosirius Red (PRS) staining was done using a commercial staining kit (#24901, PolySciences, Warrington, PA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CRUK0764 were labelled with CellVue® Red Mini Kit for Membrane Labeling (Polysciences, 25567-1) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were stained with benzidine in combination with the Differential Quick Stain Kit (PolySciences, Warrington, PA) (manufacturers protocol) ...
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bioRxiv - Cell Biology 2024Quote: Staining for collagen was performed using picrosirius red staining kit per the manufacturer’s instructions (Polysciences Inc.). Digital morphometric measurements and collagen quantitation were performed as previously described (65) ...
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bioRxiv - Bioengineering 2020Quote: ... Sections were stained with picrosirius red/hematoxylin using the Picrosirius Red Stain Kit (PolySciences, Warrington, PA, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Picrosirius Red staining of kidneys was carried out on 10μm thick sections using a kit (Polysciences 24901). Immunohistochemistry of Wilms’ Tumour (WT-1) ...
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bioRxiv - Plant Biology 2022Quote: ... The specimen was washed with acetone and embedded in Spurr resin (Spurr Low Viscosity Embedding Kit, Polysciences). Ultra-thin sections (70–80 nm ...
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bioRxiv - Genetics 2021Quote: ... Masson’s Trichrome staining was performed on frozen sections using the Masson’s Trichrome stain kit (Cat# 25088-100; Polysciences; Warrington, PA) and the following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... High resolution episcopic microscopy (HREM) was performed as previously described97 using an JB-4 embedding kit (00226-1, Polysciences GMBH, Germany). μMRI and HREM data were analysed using OsiriX MD DICOM viewer version 9.0.2 (Pixmeo) ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were then dehydrated with increasing concentrations of ethanol (50-100%),infiltrated with propylene oxide for 10 min and embedded with a Poly/Bed 812 kit (Polysciences, USA) and polymerized for 18 hours at 60°C ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Molecular Biology 2023Quote: Staining of lung sections for fibrillar collagen was performed using the Picrosirius Red Stain Kit following the manufacturer’s instructions (Polysciences, Inc., Warrington PA). Digital morphometric measurements were performed on multiple lobes and multiple levels with ten random peripheral lung images devoid of large airways per slide analyzed at a final magnification of 100× using Image J as published (34 ...
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bioRxiv - Developmental Biology 2022Quote: ... Fixed samples were gradually dehydrated in an increasing series of ethanol concentrations and were embedded in a methacrylate resin (JB-4 kit, Polysciences, Warrington, PA) containing eosin and acridin orange as contrasts agents as previously described (Mohun and Weninger ...
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bioRxiv - Molecular Biology 2023Quote: ... melanin of part of samples were breached before oil red O staining using Melanin Bleach Kit (Cat # 24883 Polysciences, Inc., Warrington, PA, USA). After washing in distilled water ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were infiltrated with propylene oxide for 10 min and embedded using a Poly/Bed 812 kit (Polysciences, Warrington, PA, USA; Cat:08792). The specimens were sectioned at 200 nm using an ultramicrotome (EM-UCT ...