Labshake search
Citations for Polysciences :
101 - 150 of 383 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... plasmids using polyethylenimine (PEI) transfection (PEI MAX® MW 40,000, Polysciences) as previously described ...
-
bioRxiv - Molecular Biology 2024Quote: ... All plasmids were cotransfected to HEK Expi293 cells by polyethylenimine (Polysciences) at 37°C for 72 hours ...
-
bioRxiv - Microbiology 2021Quote: ... and the plasmid containing a gene of interest using polyethylenimine (PEI, Polysciences). psPAX2 was a gift from Didier Trono (Addgene plasmid #12260 ...
-
bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...
-
bioRxiv - Biophysics 2021Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg polyethylenimines (PEIs, Polysciences) in 50 mL SMM 293T-II medium (Sino Biological Inc. ...
-
bioRxiv - Cell Biology 2021Quote: ... plasmid co-transfections were performed with 1mg/ml PEI MAX (24765, Polysciences) with DNA:PEI at a ratio of 1:3 as follows ...
-
bioRxiv - Molecular Biology 2020Quote: ... using 1.5 mg plasmid DNA and 7.5 mg of PEI MAX (Polysciences) in 500 mL ProCHO5 medium (Lonza) ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection into HEK 293T cells was carried out using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Invitrogen) ...
-
bioRxiv - Biochemistry 2022Quote: HEK-293T cells were transiently transfected with plasmid DNA using polyethylenimine (Polysciences) at a ratio of DNA 1:3.4 polyethylenimine ...
-
bioRxiv - Neuroscience 2020Quote: Cultured cell lines were transfected with plasmid DNA and PEI MAX (Polysciences) complexes (ratio of DNA to PEI MAX ...
-
bioRxiv - Genetics 2021Quote: ... expression plasmids into HEK293T cells using a 1mg/mL PEI solution (Polysciences). We collected lentiviral supernatants at 48 hours and 72 hours after transfection and concentrated lentiviruses by ultracentrifugation for 120 minutes at 19,500 rpm using a Beckman SW28 ultracentrifuge rotor at 4°C.
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected with CRISPR-Cas9 plasmids using PEI-Max (Polysciences Inc.) as described previously (Morciano et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with plasmid DNA and 25 kDa polyethyleneimine (Polysciences, PA) at 1 μg/mL in Freestyle 293 media ...
-
bioRxiv - Biophysics 2021Quote: ... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
-
bioRxiv - Cell Biology 2022Quote: ... packaging plasmid by using polyethylenimine (Xiang et al. 2012) (#23966-1, Polysciences) reagent as manufacturers’ instruction ...
-
bioRxiv - Microbiology 2022Quote: ... pLV-EGFP-luciferase-N and psPAX2 plasmid using polyethyleneimine (PEI, Polysciences, USA). Supernatants were harvested at 48 h and 72 h ...
-
bioRxiv - Molecular Biology 2022Quote: A standard triple plasmid transfection using polyethyleneimine (PEI)-MAX (Polysciences, MW, 40,000) method was used for AAV production as described previously (22) ...
-
bioRxiv - Biochemistry 2023Quote: ... mixed with sequence-verified plasmid DNA and PEI MAX transfection reagent (Polysciences) at a 1:3 mass ratio of DNA:PEI ...
-
bioRxiv - Molecular Biology 2024Quote: ... and transfected with plasmid DNA and PEI MAX (Polysciences, Warrington, PA, USA) complexes (DNA to PEI MAX ratio ...
-
bioRxiv - Neuroscience 2024Quote: ... Expi293F cells were transiently transfected with the plasmid and polyethylenimines (PEIs) (Polysciences). The total amount of plasmids for transfection of one liter of cell culture is 2 mg and plasmids were pre-mixed with PEIs in 50 mL fresh medium in a 1:2 (w/w ...
-
bioRxiv - Biophysics 2022Quote: ... 100 μg mL-1 streptomycin and 5% CO2 atmosphere at 370 C were transiently transfected with wild type human and rat NTSR1 in pcDNA3.1 (4 μg DNA per 75% confluent 10 cm dish) via hybridization with polyethylenimine (PEI, Polysciences, Inc.). The following day ...
-
bioRxiv - Biochemistry 2021Quote: ... 0.5mg of both plasmids were incubated with 3mg of Linear PEI 25K (Polysciences) in 100ml of Opti-MEM (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection was performed using polyethylenimine (#23966-2; Polysciences Inc., Warrington, PA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... The cells were transiently transfected with the expression plasmid and polyethyleneimine (PEI) (Polysciences) at a density of 2.0-2.5×106 cells per ml ...
-
bioRxiv - Biophysics 2021Quote: ... the cells were transiently transfected with the expression plasmids using polyethylenimines (PEI, Polysciences). In details ...
-
bioRxiv - Cell Biology 2021Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) as described previously (19 ...
-
bioRxiv - Cell Biology 2021Quote: ... The Huh7 cells were transfected with indicated plasmid using Polyethyleneimine HCl MAX (Polysciences) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) essentially as described previously (19,37) ...
-
bioRxiv - Cell Biology 2019Quote: ... the cells were transiently transfected with the expression plasmids and polyethylenimines (PEIs) (Polysciences). For expressions of the RS head component ...
-
bioRxiv - Microbiology 2023Quote: ... and gRNA containing plasmid lentiCRISPRv2 were transfected into 293T cells using polyethylenimine (Polysciences). Supernatants were collected at 48 and 72 hours post transfection ...
-
bioRxiv - Biochemistry 2022Quote: ... and the resulting plasmids were transfected into HEK293F cells using polyethylenimine (PEI, Polysciences) for expression.
-
bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells (ATCC) were transfected with plasmids using PEI ‘MAX’ (Polysciences Inc.) and subjected to electrophysiological assays 16-72 h after transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) as described previously (29 ...
-
bioRxiv - Developmental Biology 2023Quote: ... pcDNA3.0-3XFLAG-Cry2 plasmids and PEI max 40k (Polysciences Inc CN: 24765-1). 24 hours after transfection ...
-
Structural insights into perilipin 3 membrane association in response to diacylglycerol accumulationbioRxiv - Biochemistry 2023Quote: ... were transfected with 3μg of plasmid DNA/ml using Polyethylenimine HCl MAX (Polysciences) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: Plasmids were transfected in U2OS cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). 15.2 μg of plasmid and 60.8 μg of PEI were diluted in 2ml of Opti-MEM ...
-
bioRxiv - Biochemistry 2023Quote: ... The plasmid expressing CD5L was transfected into the HEK293F cells by polyethylenimine (Polysciences), and the cells were cultured in SMM 293T-I medium (Sino Biological Inc. ...
-
bioRxiv - Microbiology 2022Quote: ... and the plasmid containing a gene of interest or hairpin using polyethylimine (PEI, Polysciences). psPAX2 was a gift from Didier Trono (Addgene plasmid #12260 ...
-
bioRxiv - Microbiology 2022Quote: ... and the lentiviral transfer plasmid containing a gene of interest using polyethylenimine (PEI, Polysciences). 6 hours after transfection ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1.64 pmol respective pLKO plasmid using PEI MAX 40K (Polysciences, Inc, Warrington, PA) at a ratio of DNA to PEI 1:3 ...
-
bioRxiv - Cell Biology 2021Quote: Transfection of Capsaspora cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.). Briefly ...
-
bioRxiv - Cell Biology 2020Quote: ... envelope (pMD2-VSV) and pLenti6/V5 plasmids using linear polyethylenimine (PEI) (23966-1, PolySciences Europe ...
-
bioRxiv - Microbiology 2022Quote: ... Vero/TMPRSS2 cells were transfected with an ACE2-targeting plasmid using PEI MAX (Polysciences). At 24 h posttransfection ...
-
bioRxiv - Developmental Biology 2022Quote: ... Exogenous plasmid DNA was transfected using Transporter™ 5 transfection reagent (Polysciences, PA, USA). Co-transfection of siRNA with plasmid DNA was performed using jetPRIME® transfection reagent ...
-
bioRxiv - Systems Biology 2021Quote: Stable reporter cell lines were transfected with synTF plasmid constructs using polyethylenimine (PEI, Polysciences) as described in (Park et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... together with packaging plasmids pCMV-VSV-G and pCMV-delta8.9 using PEI (Polysciences Inc.) 48 hours after transfection ...
-
bioRxiv - Immunology 2021Quote: ... Plasmid delivery in HEK-TLR4 cells was done using linear polyethyleneimine (PEI) 25K (Polysciences) complexed with DNA in a 2:1 ratio respectively for 24 h.
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were transiently transfected with three plasmids using Polyethylenimine (PEI, Polysciences Inc. 23966-100). These plasmids were variants of pAAV-MCS ...
-
bioRxiv - Genomics 2022Quote: ... and 10µg of EF1ɑ-ligand IRES-EGFP lentiviral transfer plasmid using polyethylenimine (Polysciences Inc.) at a 3:1 PEI:plasmid ratio ...
-
bioRxiv - Immunology 2022Quote: ... 1 mg of plasmid was mixed with 4 mg of polyethyleneimine (PEI; PolySciences Inc.) in Opti-MEM cell culture medium (Thermo Scientific ...