Labshake search
Citations for Polysciences :
101 - 117 of 117 citations for RPA reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... 293T cells were co-transfected with LentiCRISPR v2 plasmids along with packaging plasmids psPAX2 and pMD2.G using PEI transfection reagent (#23966-100, Polysciences). Lentiviruses were collected 48 hours after transfection ...
-
bioRxiv - Developmental Biology 2022Quote: ... The CSII-CMV-MCS-IRES2-Bsd vector (Fbln7 FL, dCC or control eGFP) was transfected into 293T cells using polyethylenimine reagent (PEI, Polysciences Inc.) together with lentivirus packaging vectors (pRSV-Rev ...
-
bioRxiv - Cell Biology 2022Quote: 200,000 HeLa cells/35-mm well were seeded onto coverslips (Marienfeld, 630-2190) and transfected using polyethylenimine transfection reagent (PEI, Polysciences, 23966) in a 1:6 (w/w ...
-
bioRxiv - Bioengineering 2022Quote: ... and gRNA expression plasmid (6 μg) were co-transfected with transfection reagent at a 2.6/1 ratio of Polyethylenimine (PEI; Polysciences, Warrington, USA) (μg)/total DNA (μg) ...
-
bioRxiv - Cell Biology 2022Quote: ... AAV-pro 293T cells were transfected with gRNA vectors along with AAV packaging vectors AAV2/9 and pAdDeltaF6 using the PEI MAX transfection reagent (Polysciences, 24765). 72 hours after transfection ...
-
bioRxiv - Microbiology 2022Quote: ... the culture medium was removed and the cells transfected with pCMV-ORF45HA or pEGFP-C1 (Mock control) using Polyethylenimine (PEI) transfection reagent (Polysciences, Inc.). Briefly ...
-
bioRxiv - Synthetic Biology 2023Quote: ... they were transfected by mixing roughly 600 ng of plasmid mixture per well with 1.6 μL Transporter 5 Transfection Reagent (Polysciences, Warrington, PA) in 60 μL 150 mM NaCl ...
-
bioRxiv - Biochemistry 2024Quote: ... The DNA was added to 200 μl of DMEM and mixed with 7.5 μl of PEI reagent (Polysciences Inc cat# 23966). Tubes were incubated at room temperature for 15 min before adding to cells ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
-
bioRxiv - Biochemistry 2022Quote: ... Transfection was initiated by the addition of plasmid DNA and polyethyleneimine as transfection reagent (linear 25-kDa polyethyleneimine, Polysciences, Inc., Warrington, PA). Twenty-four hours post-transfection the cell cultures were diluted with an equal volume of fresh media supplemented with valproic acid (2.2 mM final concentration ...
-
bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
-
bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
-
bioRxiv - Biochemistry 2024Quote: ... cells on every dish were transfected with 15 μg of pcDNA3-HA-BACH1 using the PEI MAX reagent (24765, Polysciences, Warrington, PA). At 48 h after transfection ...
-
bioRxiv - Immunology 2023Quote: ... at a density of 2.0×106 cells/ml and co-transfected with pMlink-N-FLAG-p22phox-NOX2 and pMlink-EROS using PEI 4000 reagent (Polysciences, Warrington, PA). The transfected cells were cultured for 48 h and collected by centrifuged (4,000 × g ...
-
bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were seeded for 18–24 hr and transiently transfected with the indicated vectors using either the JetPrime reagent (Polyplus-transfection; 114-15) or polyethylenimine polymer (Polysciences Inc; 23966-1), following manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Vero/TMPRSS2 cells were transfected with pCAGGS-blast-RcACE2 or pCAGGS-blast-hACE2 plasmids using the PEI MAX transfection reagent (Polysciences, Warrington, PA, USA). Transfected cells were treated with 10 μg/mL blasticidin S (Kaken Pharmaceutical ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...