Labshake search
Citations for Polysciences :
51 - 67 of 67 citations for Penicillin G since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vectors Per2::dLuc using PEI Max (Polysciences). At 48 hours post- transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... The lentivirus was produced by co-transfection of the expression vector coding Lifeact-mCherry and lentiviral packaging plasmids (pCMVdR8.91 and pMD2.G) using PEI MAX (Polysciences #24765) into HEK293T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were co-transfected with LentiCRISPR v2 plasmids along with packaging plasmids psPAX2 and pMD2.G using PEI transfection reagent (#23966-100, Polysciences). Lentiviruses were collected 48 hours after transfection ...
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bioRxiv - Microbiology 2023Quote: ... Lenti-X cells were transfected with third-generation lentiviral mix (pMDLg/pRRE, pRSV-REV, pMD2.G) and dCas9-KRAB (blastR) with polyethylimine (Polysciences). Supernatant was harvested at 48 and 72 hours and 0.45 um filtered before applying to iSLK cells for 48 hours ...
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bioRxiv - Bioengineering 2021Quote: ... and packaging plasmids pCMV-dR8.91 (900 ng) and pCMV-VSV-G (100 ng) with 12 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences 24765-1) into HEK293T cells that had been grown to 60-80% confluence in 6-well plates ...
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bioRxiv - Cell Biology 2019Quote: ... Three-micron sagittal sections were stained with Alcian Blue Hematoxylin / Orange G (ABHOG) or picrosirius red stain (Polysciences Inc, Warrington PA). Frozen samples were fixed overnight ...
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bioRxiv - Cell Biology 2022Quote: HEK-293T cells were transfected with DNA mixes containing lentiviral transfer plasmids, pCRV1-Gag-Pol (Hatziioannou et al., 2004) and pHCMV-VSV-G (Yee et al., 1994) using polyethylenimine (PEI, Polysciences, 23966) in a 1:3 μg DNA/PEI ratio in non-supplemented DMEM ...
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bioRxiv - Molecular Biology 2023Quote: ... Producer cells were transfected with a solution containing the selected lentiviral vector transgene backbone, the packaging plasmids pMDLg/pRRE and pCMV.REV, pMD2.G (Plasmid Factory, Bielefeld, Germany) and polyethylenimine (PEI) (24765, Polysciences, Warrington, US). Transfection media was changes after 4 hours ...
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bioRxiv - Microbiology 2019Quote: ... were co-transfected with 3 µg Gibson Assembly product DNA plus 330 ng pHEF-VSV-G using polyethylenimine (Polysciences, Inc., Warrington, PA) at a ratio of 1 µg total DNA to 4 µg polyethylenimine in 800 µl of 150 mM NaCl [72] ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... Pico- and nano-phytoplankton abundance was measured on unstained samples with fluorescent beads for parameter normalization (0.95 G Fluoresbrite® Polysciences, Warrington, PA), while heterotrophic cell enumeration was performed using SYBR Green® staining as described in (Marie et al ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.625 µg of pMD2.G) using 15 µL of 1 mg/mL transfection grade linear polyethylenimine hydrochloride (MW 40,000) (PEI-MAX-40K) (Polysciences, Warrington, PA, United States) in 500 µL of Opti-MEM ...
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bioRxiv - Cancer Biology 2023Quote: ... 293 T cells were cultured to a degree of 80%-90% and co-transfected with the three-plasmid system (packaging vectors psPAX2, envelope plasmid pMD2.G, and CAR plasmid) using polyethyleneimine (Polysciences, PEI MAX 40000). At 6-8 h of post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... These vectors along with lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293FT cells by polyelthylenimine (Polysciences, Inc; 60 μg/mL) mediated transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Evolutionary Biology 2023Quote: 1,5×105 Cells were seeded on 15 mm round glass coverslips coated with polyethylenimine (PEI) solution (25 g/ml PEI, Polysciences Inc. and 150 nM NaCl) for 48 hours ...