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Citations for Polysciences :
1 - 50 of 166 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... with modified pCW57.1 target vector in a ratio of 5:1:5:5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966) as transfection reagent ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested five days after transfection ...
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bioRxiv - Biophysics 2022Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... Constructs were transfected together into HEK293 cells using polyethylenimine (PEI, Polysciences) with a 24-h incubation time for protein expression ...
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bioRxiv - Bioengineering 2023Quote: ... Membrane associated receptor candidates were transfected by polyethylenimine (PolySciences # 23966). Cells were seeded on Neuvitro Poly-D-lysine coated sterile German glass coverslips (Fisher Scientific #NC0343705 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 or 293T cells were performed using polyethylenimine (PEI) (Polysciences) transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 and U2OS cells were transiently transfected using PEI-Max Transfection reagent (Polysciences, 24765) and optimem (gibco ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Biochemistry 2020Quote: ... HDX-MS and MALS analysis were expressed in HEK293-GnT1 suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transiently co-transfected with lentiviral vectors using PEI MAX reagent (Polysciences, Warrington, PA, USA). Four hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Four retrograde tracers were employed for a total of 29 injections: (i) cholera toxin subunit B (CTB; Polysciences Inc, Eppelheim, Germany), (ii ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: HEK293 or HEK293T cells were transfected with plasmids expressing GFP-fused NCAN using Lipofectamine 3000 (Thermo) or PEI Max (Polysciences). After three or four days ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were transfected with indicated receptor constructs (3.5 μg) and βarr2 (βarr2WT/DM) constructs (3.5 μg) using polyethylenimine (PEI) linear (Polysciences, Cat no. 19850) at a ratio of 1 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Canada) and transiently co-transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimin (PEI; Polysciences Inc., USA). PEI was dissolved in water as 1 mg/mL stock solution with pH of 6.5–7.5 and kept at −20°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transporter 5™ reagent (Polysciences, Inc, Cat #26008-5) was used to transfect parental HEK293T/17 cells ...
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bioRxiv - Biochemistry 2023Quote: ... -MCM9 M45E or -GFP control (Transporter 5, Polysciences 26008-5) and harvested 3 days later for HA-immunoprecipitation as previously described39 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Montreal, Canada) and transiently transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimine (Polysciences Inc., Warrington, PA, US) in Cellstar® PS 96-well cell culture plates (Greiner Bio-One ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Fast Blue (5%, Polysciences) or rAAV-hSyn-EGFP (1012vp/ml ...
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bioRxiv - Neuroscience 2021Quote: ... or Aquamount (#18606-5, Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The day after seeding, pcDNA3L-His-CAMYEL plasmid were transiently transfected into cells using linear polyethyleneimine (PEI, m.w. 25 kDa) (#23966, Polysciences, Warrington, PA, USA). The DNA-PEI complex mixture was added into the cells at the ratio of 1:6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL PEI (Polysciences, #23966-1) in 50 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescent polychromatic beads (Polysciences, 19507-5) with a 0.5 μm particle size were added to each well at a final concentration of 3.64×108 beads/mL and incubated at 20°C for 10 minutes with shaking ...