Labshake search
Citations for Polysciences :
1 - 50 of 639 citations for N acetylglucosamine 1 phosphodiester alpha N acetylglucosaminidase NAGPA Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... Expression plasmids (Nsp1/Nsp2/N) and PEI (Polysciences, 260008-5) were separately diluted in Opti-MEM1 (Gibco 31985-047 ...
-
bioRxiv - Bioengineering 2024Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) was obtained from Polysciences Inc (Warrington ...
-
bioRxiv - Microbiology 2020Quote: ... N-(3-Aminopropyl) methacrylamide hydrochloride (APMA, >98%) was obtained from Polysciences Inc ...
-
bioRxiv - Bioengineering 2020Quote: ... was covalently functionalized with N-(3-Aminopropyl)methacrylamide (APMAm; Polysciences, Warrington, PA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
-
bioRxiv - Microbiology 2022Quote: ... pLV-EGFP-luciferase-N and psPAX2 plasmid using polyethyleneimine (PEI, Polysciences, USA). Supernatants were harvested at 48 h and 72 h ...
-
bioRxiv - Bioengineering 2024Quote: ... nominal spring constant 0.08 N/m) were calibrated using the thermal method and one 25-μm diameter polystyrene sphere (07313-5, Polysciences) was affixed to the end of each cantilever with a small amount of UV-cured glue (AA 349 ...
-
bioRxiv - Biochemistry 2020Quote: ... All protein encoding sequences were subcloned to pCAG vector with N-terminal FLAG-tag for expression in HEK293F cell lines transfected by polyethylenimines (PEIs, Polysciences). Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc. ...
-
bioRxiv - Genomics 2024Quote: ... CaGHV-1 RTA was expressed as an N-terminally 3xFLAG-tagged protein in HEK293T cells by transfecting the cells with PEI (Polysciences). The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega ...
-
bioRxiv - Immunology 2023Quote: ... at a density of 2.0×106 cells/ml and co-transfected with pMlink-N-FLAG-p22phox-NOX2 and pMlink-EROS using PEI 4000 reagent (Polysciences, Warrington, PA). The transfected cells were cultured for 48 h and collected by centrifuged (4,000 × g ...
-
bioRxiv - Biochemistry 2024Quote: ... was expressed with a TEV protease cleavable N-terminal 6xHis-tag and IgK secretion signal in HEK293 GnTI-cells upon transient transfection with PEI MAX (Polysciences Inc., 24765). The IgK secretion signal allowed for the secretion of LyLAP recombinant protein ...
-
bioRxiv - Bioengineering 2022Quote: ... and 20 μm FITC (Polysciences Inc., Warrington, USA) particles were suspended in deionized water to make up a final concentration of 1 × 106 beads/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... 2500 sterilized FITC-tagged microspheres (Polysciences Inc., PA, USA) suspended in a 100µl of PBS were slowly injected into the CCA using a 33G hypodermic needle (TSK Laboratory International ...
-
bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
-
bioRxiv - Zoology 2024Quote: ... Exsheathed larvae and adult worms were cultured in the presence/absence of 0.5 mm FITC labelled and/or 6 mm PE labelled fluorescent beads (Polysciences) for 5 days at 0.5µM (1 x107 beads/ml) ...
-
bioRxiv - Neuroscience 2021Quote: ... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections stained with antibodies against MBP or pMLC2/Pan-NF were placed in Liberate Antibody Binding (L.A.B. Polysciences Inc.), after the initial PBS wash ...
-
bioRxiv - Biophysics 2020Quote: ... and GFP in 1:1:1 ratio using polyethyleneimine (54) (Polysciences, Inc.; 23966-1; lot: 705149). We verified each construct by sequencing before and after subcloning ...
-
bioRxiv - Neuroscience 2023Quote: ... at a 1:1:1 ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum free DMEM at 16 hours post transfection ...
-
bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
-
bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
-
bioRxiv - Microbiology 2023Quote: ... and pHelper (CellBio Labs) at a 1:1:1 ratio using polyethyleneimine (PolySciences). Supernatant was collected 72hrs post-transfection ...
-
bioRxiv - Neuroscience 2024Quote: ... at a 1:1:1 molar ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum-free DMEM at 16 hours post-transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
-
bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
-
bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
-
bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
-
bioRxiv - Cell Biology 2020Quote: ... in a ratio of 3:2:1 using 1 mg/ml polyethylenimine (Cat#23966-1, Polysciences, Inc.). Retrovirus was harvested from the medium 48 h post-transfection ...
-
bioRxiv - Biochemistry 2021Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... 3 mL PEI (1 mg ml−1, pH 7.4, Polysciences) was added to 50 mL hybridoma media (Invitrogen ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 1% glutaraldehyde (Polysciences) in 0.1 M cacodylate buffer (Polysciences ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
-
Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
-
bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
-
bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
-
bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...
-
bioRxiv - Neuroscience 2023Quote: 1% Fast Blue in sterile dH2O (17740-1, Polysciences, Warrington, PA) was used as a fluorescent retrograde neuronal tracer to identify gastrointestinal-projecting sensory neurons using a modified method as previously described (76) ...
-
bioRxiv - Cell Biology 2023Quote: ... and HA-ApoER2 or FLAG-ApoER2 at a 1:1:1 ratio in DMEM using polyethylenimine (PEI 25k, Polysciences). After 3-4 h of transfection ...
-
bioRxiv - Neuroscience 2020Quote: ... and light chains (CA5-VL(X3)_pHL_sec) at a 1:1 ratio using polyethyleneimine (PEI, 1 mg/ml, MW 40,000, Polysciences Cat.# 24765) transfection reagent ...
-
bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
-
bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
-
bioRxiv - Systems Biology 2023Quote: ... along with 10 uL of 1 mg/mL PEI (Polysciences 24765-1) in 200 uL of serum-free DMEM ...
-
bioRxiv - Biochemistry 2024Quote: ... 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences, #23966-1) diluted in 18.8 mL Opti-MEM® ...
-
bioRxiv - Cell Biology 2021Quote: ... PEI (Polysciences, 23966-1) or lipofectamine 2000 (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... (PEI; Polysciences, 24765-1), co transfection were at 1:1 (4 µg:4 µg) ...
-
bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
-
bioRxiv - Neuroscience 2021Quote: ... This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media, Polysciences, Inc. ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 1% tannic acid and 1% uranyl acetate (Polysciences Inc. ...
-
bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...