Labshake search
Citations for Polysciences :
201 - 250 of 385 citations for Mouse BEX1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... triple plasmid transfection of 293 adherent cells was performed with a polyethylenimine (PEI)-MAX (Polysciences, MW, 40,000) to DNA ratio of 3:1 (a mass-per-mass ratio) ...
-
bioRxiv - Biophysics 2020Quote: ... plasmids encoding DISP1-A-NNN-SBP were transfected into the HEK293F cells using linear polyethylenimines (PEIs, Polysciences). Both DISP1-A and DISP1-A-NNN were purified essentially as previously described[17] ...
-
bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
-
bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
-
bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... A ratio of 1.2 µg Plasmid DNA per 106 cells and 40 kDa linear PEI MAX (Polysciences) transfection reagent was used for protein expression ...
-
bioRxiv - Molecular Biology 2023Quote: Equal numbers of HEK293T cells were seeded and the different plasmids were transfected with linear polyethylenimine (Polysciences), accordingly to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... and 8µg custom generated lentiviral CMV-PR or 2x/4x/6xPRE-GFP plasmids using PEI (Polysciences, #23966). Medium was refreshed after 24 hours ...
-
bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
-
bioRxiv - Microbiology 2023Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) or TransIT-293 (Takara ...
-
bioRxiv - Immunology 2023Quote: ... plasmids encoding HLA class I/II alleles [46] were transfected in HEK293T cells with PEI MAX (Polysciences).
-
bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK-293T cells were co-transfected with 100 ng of corresponding plasmids using linear PEI (Polyethyleneimine, Polysciences). 24h after transfection ...
-
bioRxiv - Microbiology 2023Quote: ... SIRT3 and scramble control under U6 promoter together with helper plasmid using PEI Max 40000 (Polysciences USA). Next day transfection medium was removed ...
-
bioRxiv - Biochemistry 2023Quote: ... The plasmids expressing Fcα–J and FcRL4 was co-transfected into the HEK293F cells by polyethylenimine (Polysciences). The cells were cultured at 37 °C ...
-
bioRxiv - Microbiology 2024Quote: HIV-1 Gag-iFRET plasmid DNA was transfected into HEK293T cells using a PEI transfection method (Polysciences) and into Jurkat cells using Lipofectamine LTX transfection reagent (Invitrogen) ...
-
bioRxiv - Neuroscience 2024Quote: ... plasmids were diluted in DMEM and incubated for 20 min at RT with Polyethylenimine (PEI, 23966, Polysciences) at a ratio 1:3 DNA:PEI ...
-
bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
-
bioRxiv - Developmental Biology 2021Quote: ... The cells were transiently transfected with ADAMTS6 WT or ADAMTS6 EA expression plasmids using PEI MAX (24765; Polysciences) and were co-cultured with Fbn1-/- mouse embryo fibroblasts (MEFs ...
-
bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...
-
Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
-
bioRxiv - Microbiology 2021Quote: HEK 293T or HeLa cells in 10-cm dishes were infected at 5 p.f.u./cell for 8 h or transfected overnight with the specified epitope-tagged plasmids using polyethylenimine (PEI, Polysciences, 2 μl of 1 mg/ml stock per μg of plasmid DNA) ...
-
bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
-
bioRxiv - Microbiology 2020Quote: ... Then cells were transiently transfected with 2mg expression plasmid per 1 liter using the Polyethylenimine (PEI) MAX (Polysciences) at a density of 1.0 x 106 cells/ml in fresh Union 293 medium ...
-
bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
-
bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
-
bioRxiv - Immunology 2022Quote: ... The S2P or S6P pCMV plasmids were used to transiently transfect HEK293F cells with polyethylenimine (PEI) (Polysciences, #24765). The recombinant S6P was affinity purified from the cell supernatant by using StrepTactin resin (IBA) ...
-
bioRxiv - Cell Biology 2019Quote: ... MDCK-II cells were transfected with 1 μg plasmid DNA per 106 cells using either polyethlyenimine (Polysciences Inc) or Lipofectamine-2000 (ThermoFisher Scientific) ...
-
bioRxiv - Systems Biology 2021Quote: ... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...
-
bioRxiv - Immunology 2020Quote: ... 1 μg of plasmid DNA was mixed with 4 μg of 25,000-molecular-weight polyethylenimine (PEI; PolySciences Inc.) in 66.67 μl Opti-MEM cell culture medium (Gibco) ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293 Freestyle™ were transiently transfected at a density of 2.5 106 cells/mL in 100mL Freestyle medium (Thermo-Fisher) by addition of 150 μg plasmid and 1.8 mL of linear polyethylenimine (PEI, 0.5 mg/ml) (Polysciences). After 24h ...
-
bioRxiv - Immunology 2020Quote: ... pEE12.4-RBD-SpyTag-Histag plasmids were transfected with PEI (PEI MAX-Transfection Grade LinearPolyethylenimine Hydrochloride, Polysciences 24765-1) into 293F cells ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
-
bioRxiv - Neuroscience 2021Quote: ... plasmids for these proteins were co-transfected with AnkR-GFP in HEK293T cells using PEI Max (Polysciences, 24765) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: Plasmid DNA encoding the heavy and light chains of recombinant monoclonal antibodies and polyethylene imine (Polysciences, Warrington, PA) were mixed at a 1:4 ratio ...
-
bioRxiv - Physiology 2022Quote: ... 16 μg AAV-U6-sgRNA-TBG-Cre plasmid and 144 μl of 1 mg/ml Polyetherimide (PEI) (Polysciences) was mixed in 9 ml OptiMEM (Thermo) ...
-
bioRxiv - Bioengineering 2022Quote: ... 750 ng of ABEs and 250 ng of sgRNA expression plasmids were co-transfected using polyethyleneimine (PEI, Polysciences) following the manufacturer’s recommended protocol ...
-
bioRxiv - Biochemistry 2023Quote: HEK cells were transiently transfected at 80% confluency with CibN and CryC plasmids using polyethylenimine (PEI) (Polysciences, Inc.). Plasmids and PEI were diluted in Opti-MEM (Gibco ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with 5 µg plasmid DNA and 15 µg Polyethylenimine (PEI 25K™, Polysciences, 23966-100), both previously prepared in OptiMEM (Gibco ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
-
bioRxiv - Biochemistry 2024Quote: HELBKO HEK293T cells were transfected with plasmids encoding pcDNA5/FRT/TO-SFB-HELB variants using PEI (Polysciences, Inc). After 24 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... Human embryonic kidney 293A cells were then used to produce full length whole Ab 1116NS19.9 or Ab 5b1 from their respective p3BNC expression plasmids that were transfected with polyethylenimine reagent (PEI; Polysciences). Functionality of cloned secreted 1116NS19.9-hIgG1 and 5b1-hIgG1 antibodies was tested by ELISA against antigen-coated plates.
-
bioRxiv - Biophysics 2021Quote: ... Cells in a single dish were transfected with 1.25 μg of c-term EGFP tagged hTMEM175 plasmid using 3.75 μg of PEI 25 k (Polysciences, Inc). Electrophysiological recordings were performed 48-72 hr after transfection ...