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Citations for Polysciences :
1 - 50 of 587 citations for Mouse Anti Human Immunodeficiency Virus HIV 1 p17 1981 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Cell Biology 2020Quote: Third-generation VSV.G pseudotyped HIV-1 lentiviruses were produced by polyethylenimine (PEI, Polysciences #23966-2)-transfection of HEK293T cells and packaged with VSVG-coats using established methods ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: HIV-1 Gag-iFRET plasmid DNA was transfected into HEK293T cells using a PEI transfection method (Polysciences) and into Jurkat cells using Lipofectamine LTX transfection reagent (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... 1 milligram of plasmid DNA encoding HIV-1 Env per 1 liter of cells was diluted in DMEM and mixed with polyethyleneimine (PEI, Polysciences, Inc.). The DNA was allowed to complex with PEI for 25 min and then cells were added ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Immunology 2023Quote: HEK293T cells at 60-70% confluence were transiently transfected with HIV-1 ADA gp145 Env using polyethylenimine (PEI Max, Polysciences) in a 1:3 ratio of plasmid to PEI (weight to weight) ...
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bioRxiv - Microbiology 2019Quote: ... Infectious full-length and chimeric HIV clones were prepared by linear polyethylenimine 25K (Polysciences), Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Immunology 2023Quote: ... plasmids encoding for HIV Env SOSIP trimers were cotransfected in HEK293F cells using PEI-MAX 4000 transfection reagent (Polysciences, Inc.). After transfected cells were incubated for four days ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 1 M anti-digoxygenin coated polystyrene beads (Polysciences) were conjugated to the peptide construct through λ-DNA of the peptide construct by incubation in a buffer containing 10 mM Tris HCl pH 7.5 and 150 mM NaCl for 30 minutes ...
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bioRxiv - Biophysics 2019Quote: ... 1 μM anti-digoxigenin coated polystyrene beads (Polysciences), diluted in a buffer containing 10 mM Tris HCl pH 7.5 and 50 mM NaCl were added to the imaging chamber and incubated for 30 min ...
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Biophysics 2024Quote: ... we diluted 1 μm anti-digoxigenin-coated polystyrene beads (Polysciences) in nucleosome dilution buffer and added it to the imaging chamber for 30 minutes ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Immunology 2020Quote: ... at 10,000 cells in 100μl PBS and human serum opsonised Fluoresbrite™ Yellow Green Microspheres 1μm (Polysciences) were added at a concentration of 10:1 for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
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bioRxiv - Immunology 2021Quote: ... Env plasmids were co-transfected in Human Embryonic Kidney 293T or GnT1-293S cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid (23 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant truncated human TSEN complexes were produced in human embryonic kidney (HEK) 293F cells by cotransfection using linear polyethyleneimine (PEI) (MW 25000, Polysciences) and incubated for 72 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Immunology 2024Quote: ... Env plasmids were co-transfected with MuLV Gag plasmid and pMV-Rev 0932 in human embryonic kidney 293T (or GnT1-) cells using polyethyleneimine (PEI Max, Polysciences, Inc.). 48h later ...
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bioRxiv - Biophysics 2020Quote: ... and GFP in 1:1:1 ratio using polyethyleneimine (54) (Polysciences, Inc.; 23966-1; lot: 705149). We verified each construct by sequencing before and after subcloning ...
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bioRxiv - Neuroscience 2023Quote: ... at a 1:1:1 ratio using PEI-polyethyleneimine “max,” (Polysciences #24765). Media was changed to serum free DMEM at 16 hours post transfection ...
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bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... mUNC80 and HA-mUNC79 plasmids at a ratio of 1:1:1:1 using polyethylenimine (PEI 25K, Polysciences) and incubated for 24h before recording ...
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bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
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bioRxiv - Microbiology 2023Quote: ... and pHelper (CellBio Labs) at a 1:1:1 ratio using polyethyleneimine (PolySciences). Supernatant was collected 72hrs post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
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bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
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bioRxiv - Biophysics 2019Quote: ... anti-digoxigenin coated polystyrene beads (Polysciences) were attached to the immobilized construct by incubation in a buffer containing 10 mM Tris HCl pH 8 and 50 mM NaCl for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... or Bouin’s fixative (Polysciences; anti-pMRLC) (Westerfield ...
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bioRxiv - Cell Biology 2020Quote: Ten million mouse embryonic fibroblasts (ATCC) were transfected with 90uL of Polyethylenimine (PEI MAX #24765 Polysciences) diluted in 240uL NaCl 150mM and 15ug of the pX335-gRNA and pUC19-homology arms-mCherry plasmids also diluted in 240uL NaCl 150mM ...
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bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...