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Citations for Polysciences :
1 - 50 of 55 citations for Mouse Anti Dengue Virus Pan Serotype NS1 Protein Antibody FG10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
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bioRxiv - Neuroscience 2020Quote: ... All other AAVs were prepared in large scale HEK293T cell cultures that were transfected with mixture plasmids of AAV encoding full-length cDNAs and the serotype DJ packaging plasmids pHelper and pRC-DJ with polyethyleneimine (Polysciences). 48 h after transfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Immunology 2020Quote: ... the PolyLink Protein Coupling Kit (Polysciences) was used as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: PolyLink Protein Coupling Kit (Polysciences, Inc) was used to prepare 50µl of fluorescence beads solution ...
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bioRxiv - Biophysics 2019Quote: ... we used a protein coupling kit (Polysciences) to coat micro-spheres with WGA14 ...
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bioRxiv - Biophysics 2021Quote: ... via Carbodiimide reaction (PolyLink Protein Coupling Kit, Polysciences Inc.). Briefly ...
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bioRxiv - Biophysics 2020Quote: ... with anti-digoxigenin antibodies from Sigma-Aldrich using a carbodiimide reaction (Poly-Link Protein Coupling Kit, Polysciences Inc.) The protein coated beads were prepared by mixing 50 ng of MBP ...
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bioRxiv - Neuroscience 2021Quote: ... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PolyLink Protein Coupling Kit (for COOH microparticles, Polysciences, Catalogue number 24350-1) was used to coat COOH - functionalized microspheres (31.7 μm diameter ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Biophysics 2019Quote: ... anti-digoxigenin coated polystyrene beads (Polysciences) were attached to the immobilized construct by incubation in a buffer containing 10 mM Tris HCl pH 8 and 50 mM NaCl for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... or Bouin’s fixative (Polysciences; anti-pMRLC) (Westerfield ...
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bioRxiv - Cell Biology 2020Quote: Ten million mouse embryonic fibroblasts (ATCC) were transfected with 90uL of Polyethylenimine (PEI MAX #24765 Polysciences) diluted in 240uL NaCl 150mM and 15ug of the pX335-gRNA and pUC19-homology arms-mCherry plasmids also diluted in 240uL NaCl 150mM ...
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Neuroscience 2020Quote: IgSF8-Fc and Tenascin-R-Fc proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Systems Biology 2021Quote: ... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids for these proteins were co-transfected with AnkR-GFP in HEK293T cells using PEI Max (Polysciences, 24765) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and rabbit anti-neuron-specific enolase (Polysciences). Secondary hydrogen peroxidase-conjugated antibodies ...
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bioRxiv - Immunology 2020Quote: Samples from NHP were depleted using purified DENV or BSA control protein adsorbed onto Polybeads polystyrene microspheres (Polysciences, Inc.) as reported earlier (11 ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Microbiology 2021Quote: ... 1 M anti-digoxygenin coated polystyrene beads (Polysciences) were conjugated to the peptide construct through λ-DNA of the peptide construct by incubation in a buffer containing 10 mM Tris HCl pH 7.5 and 150 mM NaCl for 30 minutes ...
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bioRxiv - Biophysics 2019Quote: ... 1 μM anti-digoxigenin coated polystyrene beads (Polysciences), diluted in a buffer containing 10 mM Tris HCl pH 7.5 and 50 mM NaCl were added to the imaging chamber and incubated for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... Slit2-Fc (N-(aa1-840) and C-terminal (aa841-1290) fragments) proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were produced by transient transfection of plasmids into HEK 293F cells using the polyethylenimine (PEI) Max reagent (Polysciences, Inc.) with a 1:3 ratio (wt/wt ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μg of human PANX1 plasmid and 25 μg of mouse Src Y529F mutant plasmid was used) was mixed with 150ug of PEI 25K (Polysciences) in a and incubated at room temperature for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... in a microcentrifuge tube and resuspending in coupling buffer (400 μl in first wash and 170 μl in second washing) (PolyLink Protein Coupling Kit, Polysciences Inc.). Then 20 μl of the freshly prepared EDCA solution (20 mg/ml ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Cell Biology 2023Quote: The WT and Akt2 KI mouse eyes were immersed fixed in cold 2.5% glutaraldehyde (25% glutaraldehyde stock EM grade, Polysciences, Warrington, PA) and 2% paraformaldehyde (Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen sections and plated cells were mounted following antibody staining using AquaPolymount (Polysciences, Heidelberg, Germany) and imaged using a Zeiss Apotome ImagerZ2 (Zeiss ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The sections were incubated in Liberate Antibody Binding Solution (LAB solution; Polysciences, Philadelphia, PA, USA) at room temperature for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... When staining for Aspartoacylase (ASPA) sections were incubated with Liberate Antibody Binding Solution (L.A.B., Polysciences) for 10 minutes prior to the blocking stop ...
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bioRxiv - Molecular Biology 2021Quote: 100 million cells were seeded in 15 cm dishes and the protein-DNA contacts were cross-linked using 1% (v/v) formaldehyde (Polysciences, 04018-1) for 10 min at room temperature with shaking ...
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bioRxiv - Immunology 2020Quote: ... BioInvent n-CoDeR® scFv were selected using biotinylated in-house produced recombinant LILRB3-hFc recombinant fusion proteins (captured with streptavidin-coated Dynabeads®) with or without competition or LILRB1-hFc coated to etched polystyrene balls (Polysciences, US) or plastic immunotubes ...