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Citations for Polysciences :
1 - 50 of 664 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Microbiology 2022Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells using PEI Max (Polysciences, Cat# 24765-1). At 48 h posttransfection ...
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bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Bioengineering 2023Quote: ... Membrane associated receptor candidates were transfected by polyethylenimine (PolySciences # 23966). Cells were seeded on Neuvitro Poly-D-lysine coated sterile German glass coverslips (Fisher Scientific #NC0343705 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... 170 ng of expression vectors encoding androgen receptor (AR) tagged with eGFP (eGFP-AR) or mutants were transfected per well using polyethylenimine (PEI) (Polysciences) at a ratio of 1 µg DNA to 3 µl PEI ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were transfected with indicated receptor constructs (3.5 μg) and βarr2 (βarr2WT/DM) constructs (3.5 μg) using polyethylenimine (PEI) linear (Polysciences, Cat no. 19850) at a ratio of 1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Canada) and transiently co-transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimin (PEI; Polysciences Inc., USA). PEI was dissolved in water as 1 mg/mL stock solution with pH of 6.5–7.5 and kept at −20°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were dissociated using 0.05% Trypsin with 0.53 mM EDTA (Wisent Inc., Montreal, Canada) and transiently transfected with receptor and biosensor DNA using 25 kDa linear polyethylenimine (Polysciences Inc., Warrington, PA, US) in Cellstar® PS 96-well cell culture plates (Greiner Bio-One ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen sections and plated cells were mounted following antibody staining using AquaPolymount (Polysciences, Heidelberg, Germany) and imaged using a Zeiss Apotome ImagerZ2 (Zeiss ...
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bioRxiv - Zoology 2024Quote: ... Exsheathed larvae and adult worms were cultured in the presence/absence of 0.5 mm FITC labelled and/or 6 mm PE labelled fluorescent beads (Polysciences) for 5 days at 0.5µM (1 x107 beads/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
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bioRxiv - Neuroscience 2024Quote: ... Sections stained with antibodies against MBP or pMLC2/Pan-NF were placed in Liberate Antibody Binding (L.A.B. Polysciences Inc.), after the initial PBS wash ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transiently transfected at a density of 3 × 106 cells/mL using linear polyethylenimine (Polysciences). 24 h following transfection ...
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bioRxiv - Genomics 2022Quote: ... cells are transfected using PEI (Polysciences) with 1 ug of total DNA comprising 500 ng of piggyBac (fused or unfused ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... cells using polyethylenimine (PEI) (Polysciences, Inc.) to generate the lentiviral particles ...
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bioRxiv - Genetics 2024Quote: ... into cells using PEI (Polysciences, #24765) or lipofectamine 3000 (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... 0.625 μg of plasmid DNAs per million cells followed by 2.5 μg polyethyleneimine per million cells (Polysciences) were added to the cells and gently mixed ...
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bioRxiv - Neuroscience 2023Quote: ... When staining for Aspartoacylase (ASPA) sections were incubated with Liberate Antibody Binding Solution (L.A.B., Polysciences) for 10 minutes prior to the blocking stop ...
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bioRxiv - Biochemistry 2021Quote: ... DNAs were transfected cells using polyethylenimine (Polysciences). After 48-72 h expression ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using PEI MAX (Polysciences) at a DNA:PEI ratio of 1:3.75 ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 10% paraformaldehyde (Polysciences) for 24 hours and then stained with anti-spike antibodies and plaques were counted.
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed with 4 % paraformaldehyde (Polysciences) and stained with the antibody anti-mouse KI67 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 10% formaldehyde (Polysciences). The next day ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using PEI MAX (Polysciences) at a DNA:PEI ratio of 1:3.75 ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected using PEI Max (Polysciences) and relevant expression vector ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were fixed with 4% paraformaldehyde (Polysciences) for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Microbiology 2024Quote: ... HEK-293T cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described (30 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Genomics 2024Quote: ... CaGHV-1 RTA was expressed as an N-terminally 3xFLAG-tagged protein in HEK293T cells by transfecting the cells with PEI (Polysciences). The CaGHV-1 ORF50 promoter fragments were PCR amplified and cloned into pGL4.15 luciferase reporter vector (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were mounted with Aqua Poly/Mount (Polysciences) and imaged using a Zeiss LSM510 META or LSM880 laser confocal microscope under a 40x objective ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with PEI (Polysciences, #23966-2) using 15 μg of CIGAR-seq vector ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using linear polyethylenimine (Polysciences Inc.) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using polyethylenimine (PEI, Polysciences Inc.) and 150 mM NaCl using a 1:6 (w:v ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were embedded with Aqua-Poly/Mount (Polysciences). The primary and secondary antibodies used for immunostaining are listed in Appendix Table S3.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transiently transfected with polyethyleneimine (Polysciences) with different StrepII-GFP-CSPP1 constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 37°C and permeabilized in ice-cold 90% methanol (Merck ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were transfected using Polyethylenimine MAX (Polysciences Inc.) or 293fectin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The cells were fixed with 10% formaldehyde (Polysciences) for 24 hours after which the ELISA procedure mentioned above was performed using serum from each vaccinated animal.
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...