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Citations for Polysciences :
151 - 200 of 236 citations for KHK IN 2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... This was followed by 2 minutes incubation of the slices in 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences). rinsed 5 × 2 min in chilled buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Whole larva were then mounted between a 2% M9 agarose pad and coverslip in M9 containing 0.1μm polystyrene beads (Polysciences, Inc.) and 10 mM Levamisole to induce worm paralysis (Reich et al. ...
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bioRxiv - Microbiology 2021Quote: Prior to flow cytometry analysis 2 μl of fluorescent microspheres (1×10−3 of the stock Fluoresbrite® YG Microspheres 0.75μm, Polysciences) was added and the volume was adjusted to 500 μl by adding FACSFlow solution (10 mM phosphate-buffered saline ...
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bioRxiv - Immunology 2020Quote: Mice were given a single oral gavage of 2 × 1011 of Fluoresbrite Yellow Green labelled 200 nm microbeads (Polysciences, Hirschberg an der Bergstrasse ...
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bioRxiv - Molecular Biology 2022Quote: L1 and L4 larvae were mounted on a 10% agarose pad and immobilized in a 1-2 μL suspension of 0.1 μm polystyrene beads (Polysciences), as described in (Kim et al ...
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bioRxiv - Biophysics 2022Quote: ... The cells in each well were transiently transfected with 1 μg HcKCR1/2-CGFP DNA plasmid using 3μg linear polyethylenimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with 1 μg/ml of purified plasmid DNA and 2 μg/ml of polyethylenimine (Polysciences Inc.). Cells were harvested 72 hours post transfection and frozen at −80°C until used ...
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bioRxiv - Genetics 2019Quote: ... a kind gift from the Nolan lab (https://web.stanford.edu/group/nolan/_OldWebsite/retroviral_systems/phx.html) with Polyethylenimine (PEI, Polysciences Cat. 23966-2). Viral supernatant was collected after 48 hours post transfection.
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bioRxiv - Genomics 2021Quote: ... and 2 μm-diameter fluorescent beads were added to each sample as an internal standard (Polysciences, Warminster, PA, USA). The samples were analyzed on a FACSCanto™ II Flow Cytometry Analyzer Systems (BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... were transfected with 1 μg DNA/ml cells in a 3:2 (LC:HC) ratio were transfected using polyethylenimine HCl MAX (Polysciences). After 4 to 5 days of expression ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were grown in 100 mm diameter dishes to 90% confluency and were subsequently transfected with 6 μg of pCG1_SARS-2-S plasmid using polyethylenimine (PEI) Max (MW = 40,000KDa, Polysciences, Germany) as transfection reagent ...
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bioRxiv - Cell Biology 2022Quote: ... PRMT6-HA or Pu.1 as indicated using Polyethylenimine according to the manufacturer’s protocol (PEI, Polysciences, Cat#24765-2)
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bioRxiv - Microbiology 2022Quote: Leishmania promastigotes were harvested in logarithmic growth phase and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... luciferase encoding reporter plasmid (pTG126) and plasmid encoding Wuhan strain SARS-CoV-2 S using 24 μL polyethylenimine (Polysciences) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using PolyJet™ DNA In Vitro Transfection Reagent (Signagen, #SL100688) or PEI (Polysciences Inc, 23966-2) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... MLV-GagPol/pHIV 8.1 and pHIT VSVg at a ratio of 2:3:1 for 48 h using polyethylenimine (Polysciences, Germany). 293T supernatant was collected and filtered through a 0.2-μm filter before being used to transduce MDCK II cells ...
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bioRxiv - Neuroscience 2021Quote: ... Cutaneous afferents innervating both hind paws of male C57BL/6J mice were labelled with the retrograde tracer Fast Blue (2% w/v in sterile PBS; Polysciences) when animals were aged 9 weeks ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with FACS buffer and fixed in 200 µl of 2% ultrapure formaldehyde (Polysciences, Warrington, PA) diluted in FACS buffer (fixation buffer) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Microbiology 2020Quote: Subconfluent 293T cells in a T75 flask were transfected with 15 μg of the SARS-CoV-2 S plasmid using polyethylenimine (Polysciences), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with 0.5 μg/mL 40-6-diamino-2-phenylindole (DAPI) for 10 minutes and the slides were mounted with Aqua-Poly/Mount (Polysciences).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cells in a single well of 6-well plate (confluency ≥80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Physiology 2020Quote: ... were transiently co-transfected with either A1R-Nluc or A3R-Nluc and β-arrestin2-YFP (total 2 μg, at a 1:6 ratio) using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Microbiology 2020Quote: 293T cells were grown to ~70% confluence and transfected with a mixture of 2 μg of DNA encoding the gRNA and 6 μL of Polyethylenimine (PEI, Polysciences catalogue #24765 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 3×105 cells/well (12-well plate) or 2×106 (10-cm dish) and transfected on the following day using polyethylenimine (PolySciences).
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Bioengineering 2022Quote: ... Methacrylation (20% theoretical) was performed to obtain OMA (5OX30MA) by reacting oxidized alginate with 2-aminoethyl methacrylate hydrochloride (AEMA, Polysciences). To synthesize OMA ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the pNL4-3-inGluc and S constructs in a 2:1 ratio using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) in order to generate viral particles ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Synthetic Biology 2023Quote: ... transient transfection mixtures were created by mixing 500 ng of plasmid with polyethyleneimine (PEI-MAX, linear 40 kD #24765-2, Polysciences) at 4.12 μg of polyethyleneimine per microgram of DNA in 150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vector Per2::dLuc using PEI Max (Polysciences). At 48 hours post-transfection ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vectors Per2::dLuc using PEI Max (Polysciences). At 48 hours post- transfection ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed three times in PBS for 10 minutes and coverslipped using 2 drops of Aqua-Poly/Mount (Polysciences, Inc.) as the mounting media.