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Citations for Polysciences :
551 - 600 of 608 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade B BH10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and gRNA expression plasmid (6 μg) were co-transfected with transfection reagent at a 2.6/1 ratio of Polyethylenimine (PEI; Polysciences, Warrington, USA) (μg)/total DNA (μg) ...
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bioRxiv - Microbiology 2022Quote: ... mice were fasted for two hours and then given a single dose of 1×106 10 μm Fluoresbrite® YG carboxylate microspheres (Polysciences) via oral gavage ...
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bioRxiv - Neuroscience 2022Quote: ... cell nuclei were stained by DAPI for 1 min at RT and tissues were mounted in Aqua-Poly/Mount (Polysciences, #18606). Images were acquired using Zeiss LSM 880 confocal microscope with a Zeiss 20x/0.8 Plan-Apochromat objective and later analyzed using ImageJ.
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 100 μg plasmid in complex with 250 μg PEI MAX 4000 (Polysciences, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 5.7 μg/150 mm dish of construct DNA was transfected with 22.8 μg/150 mm dish of pAAV9 capsid plasmid and 11.45 μg/150 mm dish of pAdDeltaF6 helper plasmid using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
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bioRxiv - Bioengineering 2024Quote: ... negative staining was performed by depositing for 10 minutes with the gird membrane side down on a drop of 1% (w/v) uranyl acetate (Polysciences, USA). The remaining solution on the grid was removed using filter paper (Whatman ...
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bioRxiv - Molecular Biology 2024Quote: ... was co-transfected with a pcDNA5/FRT/TO plasmid containing the gene of interest in a 1:10 ratio using polyethylenimine “MAX” (Polysciences, 24765). After medium exchange 6 h post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells grown in 10 ml of medium (3.0 x 106 cells/ml) were transfected with 6 µg of pCAGGS-NP (1-395) using Polyethyleneimine MAX (Polysciences, Warrington, PA, USA). Three days post-transfection ...
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bioRxiv - Bioengineering 2021Quote: ... DexMA was dissolved at 0.5 g mL-1 in a 1:1 mixture of milli-Q water and dimethylformamide with 1% (w/v) Irgacure 2959 photocrosslinker and 0.625 mM methacrylated rhodamine (Polysciences, Inc., Warrington, PA). For matrix displacement studies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then transfected with 100 µg plasmid in complex with 250 µg PEI MAX 4000 (Polysciences, Inc, Cat. No. 24765-1). Cell culture supernatants were collected at 48 to 72 hours post transfection ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
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bioRxiv - Immunology 2022Quote: ... the next day cells were first transfected with 1 μg/well of the paT7 plasmids using polyethyleneimine (PEI) (Polysciences, Inc. Warrington, PA); 2 hours later ...
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bioRxiv - Cancer Biology 2022Quote: ... or HEK293T cells were seeded for 24h and transiently transfected with the indicated vectors using either polyethyleneimine polymer (Polysciences Inc; 23966-1) TransIT-X2 Dynamic Delivery System (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells grown to 70-80% confluency were transfected with 6 μg ITR-containing plasmid and 22 μg pDGM6 using 112 μg acidified PEI (Polysciences, 23966-1). 48-72 hours after transfection ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... histological analysis of periodic acid-Schiff stain was performed on mouse kidney sections using PAS Stain Kit (24200-1, Polysciences, Warrington, PA). Images were visualized at 25× magnification using Leica TCS SP8 (Leica Microsystems ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Immunology 2020Quote: ... to 100 μL and then added to 6.0 μg of polyethylenimine (PEI) (1 mg/mL, linear, 25 kDa; Polysciences, Warrington, FL, USA) diluted in PBS to 100 μL (3:1 ratio of PEI to DNA (w/w)) ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Biophysics 2022Quote: ... Solutions were then placed into fresh tubes along with a single polystyrene bead (1/8 inch Polyballs, Cat. No. 17175; Polysciences, Warrington, PA) and agitated again for 30 min to remove trace hexadecane still present in solution ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transiently transfected with GFP-tagged versions of the hnRNPDL-1/-2/-3 isoforms and linear polyethylenimine (PEI; Polysciences, Eppelheim, Germany) in a 1:3 DNA:PEI ratio ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... 100-200 µg of purified bacmid in 1 mL water were incubated with 400 µg of 25000 MW polyethyleneimine (PEI; Polysciences Cat# 23966) in 1 mL water at 55 degC for 30-45 minutes to sterilize ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Microbiology 2022Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells using PEI Max (Polysciences, Cat# 24765-1). At 48 h posttransfection ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for 1 min and coverslips were mounted onto SuperFrost® Plus slides (R. Langenbrink) with fluorescent Aqua-Poly/Mount mounting medium (Polysciences, 18606-20). The cells were imaged using a Leica TCS SP5 confocal laser scanning microscope and an HCX PL APO lambda blue 63× oil UV objective or HCX PL APO 40.0×1.30 OIL objective controlled by the LAS AF scan software (Leica Microsystem ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were seeded for 18–24 hr and transiently transfected with the indicated vectors using either the JetPrime reagent (Polyplus-transfection; 114-15) or polyethylenimine polymer (Polysciences Inc; 23966-1), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with the transfection solution that was prepared by combining 8 µL (per dish, hereafter) polyethyleneimine (PEI) solution (1 mg/mL; Polysciences, Warrington, PA, USA) and a plasmid mixture consisting of 400 ng human LPAR-encoding plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... and 0.625 µg of pMD2.G) using 15 µL of 1 mg/mL transfection grade linear polyethylenimine hydrochloride (MW 40,000) (PEI-MAX-40K) (Polysciences, Warrington, PA, United States) in 500 µL of Opti-MEM ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus was produced in HEK293T cells by transfecting plasmid DNA of interest with packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences, Cat# 23966-1). Media were replaced 6-8 hrs following transfection ...
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bioRxiv - Immunology 2023Quote: ... Thermo Fisher Scientific) for 1 hour after which the coverslips were mounted on slides with aqua-poly/mount (Aqua-Poly/Mount, Polysciences, Warrington, PA, USA) and analyzed by fluorescence microscopy ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ug pHelper plasmid into one 15-cm dish of confluent HEK-293T cells using PEI-Max (Polysciences Inc. catalog # 24765-1). After transfection the next day we changed the medium to 1% FBS ...
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bioRxiv - Immunology 2023Quote: ... we labeled circulating monocytes by injecting the mice retro-orbitally with 1 µm diameter Flouresbrite flash red plain microspheres beads (Polysciences Inc, Warrington, PA) diluted in sterile PBS 24 hours before harvesting ...
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bioRxiv - Synthetic Biology 2024Quote: ... 300 frames) of intravenously perfused (15 µl/min) 1 µm large fluorescent microspheres (Yellow/Green Fluoresbrite carboxylate, Polysciences, 107 microspheres/ml 1x PBS). Speed was determined from the centerline microsphere ...
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bioRxiv - Cancer Biology 2020Quote: Lentivirus was produced in HEK293T cells by transfecting plasmids together with helper packaging plasmids (VSVG and psPAX2) using polyethylenimine (PEI 25000; Polysciences; Cat. No. 23966-1) transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... 1.5 × 108 cells expressing GFP-Nup131 were fixed in 1 mL of a mixture of 4% formaldehyde (18814-10, Polysciences, Inc, Warrington, PA, USA) and 0.01% glutaraldehyde (1909-10 ...
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bioRxiv - Neuroscience 2023Quote: ... Transfection with the plasmid expressing PH-PLCδ::GFP probe was done 24 hours before the imaging experiments using Polyethylenimine solution (PEI; Polysciences Inc.; 1 mg/ml) when the cells were around 70% confluent.
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bioRxiv - Immunology 2023Quote: ... HIV-1 Env and furin protease-encoding plasmids were mixed in a 3:1 Env to furin ratio (w/w) were incubated with PEImax (Polysciences Europe GmBH, Eppelheim, Germany) in a 3:1 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ug of the MAXI-prep DNA that was pre-diluted in 13 ml of Opti-MEM® I Reduced Serum Medium (Thermo, 31985-062) and 800 ug Polyethylenimine (PEI 25K, Polysciences CatNo 23966-1) likewise pre-diluted in 13 ml of Opti-MEM media ...