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Citations for Polysciences :
1 - 50 of 543 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and packaging plasmids pCMV-dR8.91 (900 ng) and pCMV-VSV-G (100 ng) with 12 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences 24765-1) into HEK293T cells that had been grown to 60-80% confluence in 6-well plates ...
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bioRxiv - Bioengineering 2020Quote: ... methacryloxypropyl trimethoxysilane (MAPTMS, 99%, Polysciences Inc.) was hydrolyzed with dilute HCl ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Genetics 2020Quote: ... with transfection mix composed of 100 ng of 25 kDa linear polyethylenimine (Polysciences) and DNA for GRK2-based Gα activation biosensor (40 ng of FLAG-hGIPR(WT ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed by adding 100 μl 4% paraformaldehyde (Polysciences) for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Biophysics 2021Quote: ... coated with polyethlenimine (100 µg/ml; Polysciences). Neurons were incubated in 5% CO2 at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... with 100 ng total DNA per well using polyethyleneimine (PEI; linear 25 kDa; Polysciences) at a DNA/PEI mass ratio of 1:3 ...
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bioRxiv - Neuroscience 2023Quote: ... We transfected 1000 ng of DNA per well using PEI-MAX (Polysciences # 24765-100) using a ratio of 1000 ng of DNA ...
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bioRxiv - Biochemistry 2020Quote: Plasmids (500 ng DNA per ml culture) and polyethylenimine (MW 25,000; Polysciences; 3 µg per ml culture) were mixed with OptiMEM (Gibco ...
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Bioengineering 2023Quote: ... we combined 100 ng of DNA with 1 μL of PEI MAX solution (polyethyleneimine, Polysciences) in 10 μL of DMEM and incubated at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T cells were co-transfected with 100 ng of corresponding plasmids using linear PEI (Polyethyleneimine, Polysciences). 24h after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Biochemistry 2024Quote: ... 50 ng/well in total) using linear PEI 25 kDa (1 mg/mL, Polysciences Inc.) transfection reagent ...
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... Yeast cells were then incubated with 0.01% Uvitex2B (a chitin marker; Polysciences) and mAb 18B7 (10 µg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 18 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 1,000 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2.93 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 300 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were co-transfected with the plasmids containing the bait and prey ORF (100 ng for each) pre-mixed with polyethylenimine (PEI) (Polysciences Inc.) and OptiMEM (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... and 100 ng of the G2-tagged targets (CTD, RPB1 or RPB2, respectively) using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). When indicated ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were co-transfected with both plasmids containing the Venus fragments-tagged bait and prey ORF (100 ng for each) which were pre-mixed and incubated with polyethylenimine (PEI) (Polysciences Inc.) and OptiMEM (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... or 12mm circular #1.5 coverslips (Neuvitro) coated with polyethylenimine (100 µg/ml; Polysciences). Neurons were incubated in 5% CO2 at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... followed by ∼3 microliters of the final assay solution containing 5 ng mL-1 500 nm diameter beads (PolySciences Inc,), coated nonspecifically with neutravidin18(ThermoFisher Inc.).
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bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... and LPS (100 μg/ml) coupled to 3-μm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Bioengineering 2022Quote: ... 750 ng of ABEs and 250 ng of sgRNA expression plasmids were co-transfected using polyethyleneimine (PEI, Polysciences) following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 spike gene expression vector in conjunction with lentiviral vectors p8.91 and pCSFLW using polyethylenimine (PEI, Polysciences, Warrington, USA). Pseudotype-containing supernatants were harvested 48 hours post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 500 ng of GFP-LRRK2 construct and 500 ng of GFP-Rab10 using polyethylenimine (PEI, Polysciences). After 36 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Expi293F cells at a density of 3×106 cells/mL were transfected with 1mg/L of culture of plasmid complexed with PEI Max 40K (cat. 24765-100, Polysciences) in a 1:2 plasmid:PEI w/w ratio in 40mL Opti-MEM (cat ...
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bioRxiv - Biophysics 2020Quote: ... 0.4 ng of 500 nm diameter polystyrene beads (Polysciences) were coated with 5 mg⋅mL−1 neutravidin solution (Thermo Fisher ...
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Rationally designed protein bandpass filters for controlling cellular signaling with chemical inputsbioRxiv - Synthetic Biology 2023Quote: ... and 600 ng of polyethyleneimine (24765-1, Polysciences, Inc.). For drug treatment experiments ...
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bioRxiv - Immunology 2022Quote: ... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Transfection reactions were prepared by the addition of 2-10 ug of bacmid DNA to 100 μl 1xPBS and 12 μL polyethyleneimine (PEI) at 1 mg/mL (Polyethyleneimine “Max” MW 40.000, Polysciences #24765, USA) to 100 μL 1xPBS ...