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Citations for Polysciences :
1 - 50 of 745 citations for Dengue Virus Serotype 3 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Neuroscience 2022Quote: ... and envelope plasmids using PEI (Polysciences, 24765-100). Media from transfected cells was harvested at 24 ...
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bioRxiv - Neuroscience 2024Quote: Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
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bioRxiv - Immunology 2022Quote: ... and pMD2G-VSV-G (envelope) using PEI-MAX (Polysciences). Packaged lentivirus was used to transduce 293T cells in the presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... Plat-E cells were co-transfected with target gene vectors and an amphotropic envelope vector using polyethylenimine (PEI; Polysciences, Warrington, PA, USA) as described previously39 ...
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bioRxiv - Cell Biology 2020Quote: ... envelope (pMD2-VSV) and pLenti6/V5 plasmids using linear polyethylenimine (PEI) (23966-1, PolySciences Europe ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Neuroscience 2020Quote: ... All other AAVs were prepared in large scale HEK293T cell cultures that were transfected with mixture plasmids of AAV encoding full-length cDNAs and the serotype DJ packaging plasmids pHelper and pRC-DJ with polyethyleneimine (Polysciences). 48 h after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Microbiology 2022Quote: ... carrying defective envelope of VSV plasmid (trans form) and GFP reporter plasmid by using Transporter TM5 transfection reagent (PEI, 40,000Da, PolySciences), according to the provider guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were bound to Concanavalin A beads (86057-3, Polysciences Inc), followed by permeabilization using a digitonin containing buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 250.000 cells were captured with BioMagPlus Concanavalin A (Polysciences, 86057-3) and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4 ...
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bioRxiv - Cell Biology 2020Quote: ... PlatE cells were co-transfected with the packaging plasmid pMRX-puro-tfLC3 plasmid and the envelope-encoding plasmid pVSVG by using of polyethylenimine (Polysciences Inc.). Supernatants were harvested 48 h post-transfection and filtered.
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bioRxiv - Molecular Biology 2023Quote: For HIV-1 virus growth assay pNL4-3 and its mutated versions pNL4-3 TC-1G and pNL4-3 TC-3G were transfected into freshly seeded 293T cells using polyethylenimine (Polysciences) [51] ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transiently transfected at a density of 3 × 106 cells/mL using linear polyethylenimine (Polysciences). 24 h following transfection ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... Hek293T-cells were transfected with pNL4-3 or pCH058/2960 or pNL4-3 and pMM310 (pBlam-Vpr) via polyethylenimine (Polysciences Inc.)[67] ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Cancer Biology 2023Quote: ... 293 T cells were cultured to a degree of 80%-90% and co-transfected with the three-plasmid system (packaging vectors psPAX2, envelope plasmid pMD2.G, and CAR plasmid) using polyethyleneimine (Polysciences, PEI MAX 40000). At 6-8 h of post-transfection ...
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated at 37°C for 3 days and later fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed in 3% paraformaldehyde and were stained for 1 hour with 1% Uvitex (Polysciences), which specifically binds to the chitin of the fungal cell wall ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated at 37°C for 3 days and then fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed using 3% formaldehyde and fixed cells were placed on poly-L-lysine coated multi-well slides (Polysciences Inc., Warrington, PA, USA). Cells were then blocked for one hour using phosphate-buffed saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...
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bioRxiv - Immunology 2023Quote: ... 3 μm (#19118-2; Polysciences) or 6 μm (#17145-4 ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... pNL4-3 and pNL4-3dNef were transfected into HEK293T cells using polyethylenimine (PEI) transfection reagents (Polysciences, PA, USA). At 48 h post-transfection ...