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Citations for Polysciences :
51 - 100 of 1047 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Immunology 2020Quote: ... the PolyLink Protein Coupling Kit (Polysciences) was used as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: PolyLink Protein Coupling Kit (Polysciences, Inc) was used to prepare 50µl of fluorescence beads solution ...
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bioRxiv - Cell Biology 2019Quote: ... MDCK-II cells were transfected with 1 μg plasmid DNA per 106 cells using either polyethlyenimine (Polysciences Inc) or Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2023Quote: ... the pCAGGS vectors containing the sequences encoding the immunoglobulin heavy and light chains were cotransfected into HEK293T cells at 1:1 ratio using PEI Max (Polysciences, Cat# 24765-1). The culture medium was refreshed with DMEM (low glucose ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: COS-1 cells were transfected with AcGFP-CDC42 using PEI MAX (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with either 1 mg/ml polyethylenimine (PEI; Polysciences, Inc.) or Effectene transfection reagent (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were triple-transfected using polyethylenimine Max (PEI Max, Polysciences 24765-1) with 12 μg of pAAV-helper ...
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bioRxiv - Neuroscience 2023Quote: Cells for ICC were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Microbiology 2023Quote: ... 1 million HEK293T cells were seeded into 6-well plates and transfected using polyethylenimine (PEI) (#23966-1; Polysciences, Inc.) with 1 μg of the indicated DNA constructs at a 1:5 ratio with PEI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at a 2:1:1 ratio in HEK293T cells using 3 μg polyethylenimine (25 kDa linear, Polysciences, Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were infiltrated with a 1:1 mixture of propylene oxide and Polybed 812 epoxy resin (Polysciences, Inc.) for 2 hours and infiltrated overnight ...
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bioRxiv - Biophysics 2019Quote: ... we used a protein coupling kit (Polysciences) to coat micro-spheres with WGA14 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... and wildtype b0,+AT (the pEZT-C-TwinStrep-mCherry-OaSLC7A9 plasmid) were transfected into HeLa cells at 1:1 molar ratio by using PEI MAX (40,000 MW Linear; Polysciences, Inc.). The ratio of DNA ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Synthetic Biology 2022Quote: ... were transfected with pHelper, pRepCap and pTransgene (2:1:1 ratio, 2 μg DNA per million cells) using Transport 5 transfection reagent (Polysciences) with a 2:1 PEI:DNA ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using 90 mg of PEI-Max (1 mg/mL, Polysciences) with ...
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bioRxiv - Microbiology 2019Quote: ... Agarose embedded cell pellets were post-fixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Cells in each well were transfected using polyethylenimine (PEI MAX; Polysciences 24765-1) with 0μg helper plasmid pAdΔF6 ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were transfected with 1 mg/mL polyethylenimine (PEI; Polysciences, Warrington, PA) at a ratio of PEI-to-DNA of 3:1 ...
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bioRxiv - Biophysics 2020Quote: ... Cells were transiently transfected with desired cDNAs using Polyethylenimine (1 μg/μl; Polysciences) for electrophysiological studies ...
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bioRxiv - Microbiology 2022Quote: ... Cells were transfected using 90 μg of PEI-Max (1 mg/mL, Polysciences) with ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were transfected using polyethylenimine (PEI, from Polysciences, Inc. cat. no. 23966-1) in OptiMEM (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using 90 mg of PEI-Max (1 mg/mL, Polysciences) with ...
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bioRxiv - Cell Biology 2021Quote: Frozen cells were freeze-substituted into Lowicryl HM20 resin (Polysciences Inc., 15924-1) using freeze-substitution device (Leica EM AFS2 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected using polyethylenimine (PEI) (Polysciences, 23966-2, 1 mg/mL) and 3rd generation lentivirus packaging vectors (pMDLg ...
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bioRxiv - Neuroscience 2023Quote: ... The HEK293T cells were transfected using 1 mg/mL Polyethylenimine (PEI; 23966, Polysciences) in a ratio of 3 μL to 1 μg DNA ...
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bioRxiv - Immunology 2023Quote: ... Cells were co-transfected using 90 µg PEI- Max (1 mg/mL, Polysciences) with 15 µg HIV-luciferase plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were transfected via polyethyleneimine (PEI; Polysciences, Warrington, PA, cat. no. 23966-1). The protocol was adapted from Longo et al.41 ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The cells were transfected using polyethyleneimine (PEI, MW 40,000; 1 mg/ml, Polysciences) using 2 µg of plasmid DNA ad 6 µl of PEI ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Expi293 cells were transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 x 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). 24-hours following transfection ...
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bioRxiv - Immunology 2020Quote: ... 1 milligram of plasmid DNA encoding HIV-1 Env per 1 liter of cells was diluted in DMEM and mixed with polyethyleneimine (PEI, Polysciences, Inc.). The DNA was allowed to complex with PEI for 25 min and then cells were added ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then transfected with 1 μg of JFH1-QL/GLuc2A in vitro transcribed RNA (1 μg) using polyethylenimine (Polysciences, Inc.). Supernatants were harvested and mixed with Renilla luciferase buffer and substrate ...
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bioRxiv - Biophysics 2021Quote: ... via Carbodiimide reaction (PolyLink Protein Coupling Kit, Polysciences Inc.). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with the corresponding plasmids using polyethylenimine (1 μg/μL [pH 7]; Polysciences) in a 1:3 ratio ...
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bioRxiv - Cell Biology 2021Quote: ... and pMD2.G were co-transfected into 293T cells using polyethyleneimine (PEI, Polysciences 23966-1). Virus-containing supernatant media were harvested 48 hours post transfection and passed through 0.45 µm filters ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells stably transduced with HSPB8 were transiently transfected using PEI MAX (24765-1, PolySciences Europe ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Cell Biology 2019Quote: ... into HEK 293T cells using 1mg/mL of PEI Max 40000 (Polysciences, cat. 24765-1). After 48 hours of transfection ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cross-linked with 1% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA), for 10 min at 26°C followed by another 5 min incubation after adding glycine to a final concentration of 125 mM to stop the cross-linking reaction ...