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Citations for Polysciences :
51 - 92 of 92 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Cancer Biology 2020Quote: ... complemented with 10% FCS and 2mM glutamine) were transfected using 4μg polyethylenimine (PEI, Polysciences Inc., Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...
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bioRxiv - Biochemistry 2020Quote: Plasmids encoding human EMC4 with an N- or C-terminal 3xHA tag in the pcDNA5/FRT/TO vector were transiently transfected into HEK293T cells using PEI “MAX” (Polysciences). At 3 days after transfection ...
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bioRxiv - Biochemistry 2020Quote: ... All protein encoding sequences were subcloned to pCAG vector with N-terminal FLAG-tag for expression in HEK293F cell lines transfected by polyethylenimines (PEIs, Polysciences). Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
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bioRxiv - Immunology 2020Quote: 1L of 293SGlycoDelete cells16 were cultured to a density of 1 million cells/mL and transiently transfected with 500μg of pαH-RBD-Fc using 2 mg of polyethylenimine (PEI, Polysciences, Cat# 24765). Cultural supernatant was harvested 6 days post-transfection by centrifugation and sterile filtered using a 0.22μm vacuum filter ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Immunology 2020Quote: ... the PolyLink Protein Coupling Kit (Polysciences) was used as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: PolyLink Protein Coupling Kit (Polysciences, Inc) was used to prepare 50µl of fluorescence beads solution ...
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bioRxiv - Biophysics 2019Quote: ... we used a protein coupling kit (Polysciences) to coat micro-spheres with WGA14 ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Biophysics 2021Quote: ... via Carbodiimide reaction (PolyLink Protein Coupling Kit, Polysciences Inc.). Briefly ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were transfected with 100 ng of human or rhesus ACE2 receptor plasmid DNA using polyethylenimine (Polysciences). After 24 h ...
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bioRxiv - Immunology 2020Quote: ... at 10,000 cells in 100μl PBS and human serum opsonised Fluoresbrite™ Yellow Green Microspheres 1μm (Polysciences) were added at a concentration of 10:1 for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Env plasmids were co-transfected in human embryonic kidney 293T cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid and pMV-Rev 0932 for Envs carrying native codons ...
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bioRxiv - Biophysics 2020Quote: ... with anti-digoxigenin antibodies from Sigma-Aldrich using a carbodiimide reaction (Poly-Link Protein Coupling Kit, Polysciences Inc.) The protein coated beads were prepared by mixing 50 ng of MBP ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PolyLink Protein Coupling Kit (for COOH microparticles, Polysciences, Catalogue number 24350-1) was used to coat COOH - functionalized microspheres (31.7 μm diameter ...
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bioRxiv - Immunology 2021Quote: ... Env plasmids were co-transfected in Human Embryonic Kidney 293T or GnT1-293S cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid (23 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant truncated human TSEN complexes were produced in human embryonic kidney (HEK) 293F cells by cotransfection using linear polyethyleneimine (PEI) (MW 25000, Polysciences) and incubated for 72 h at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... fused to β-arrestin2 or the human MOR (0.1 µg β-arrestin2-LgBIT cDNA, 2 µg of the hMOR-SmBIT cDNA) using polyethylenimine (PEI; Polysciences EuropeGmbH ...
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bioRxiv - Immunology 2024Quote: ... Env plasmids were co-transfected with MuLV Gag plasmid and pMV-Rev 0932 in human embryonic kidney 293T (or GnT1-) cells using polyethyleneimine (PEI Max, Polysciences, Inc.). 48h later ...
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bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: 293F cells were co-transfected with a SOSIP-expressing plasmid and a human furin-expressing plasmid at a 4:1 ratio (4 SOSIP : 1 furin) using polyethyleneimine (PEI; Polysciences, Inc. Warrington, PA). Transfected cells were grown for 3-5 days in a tissue culture incubator at 37°C ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Systems Biology 2021Quote: ... Firefly reporter and Renilla transfection control were transfected together with plasmids expressing viral proteins using polyethylenimine (PEI, Polysciences) for untreated and treated conditions ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids for these proteins were co-transfected with AnkR-GFP in HEK293T cells using PEI Max (Polysciences, 24765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Samples from NHP were depleted using purified DENV or BSA control protein adsorbed onto Polybeads polystyrene microspheres (Polysciences, Inc.) as reported earlier (11 ...
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bioRxiv - Cell Biology 2023Quote: ... 293FT cells were transfected with plasmids encoding FLAG- and/or HA-tagged proteins using Polyethylenimine Max (MW: 40,000; Polysciences). After 48 h ...
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bioRxiv - Neuroscience 2019Quote: ... Slit2-Fc (N-(aa1-840) and C-terminal (aa841-1290) fragments) proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Molecular Biology 2022Quote: Proteins were produced by transient transfection of plasmids into HEK 293F cells using the polyethylenimine (PEI) Max reagent (Polysciences, Inc.) with a 1:3 ratio (wt/wt ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Biophysics 2021Quote: ... in a microcentrifuge tube and resuspending in coupling buffer (400 μl in first wash and 170 μl in second washing) (PolyLink Protein Coupling Kit, Polysciences Inc.). Then 20 μl of the freshly prepared EDCA solution (20 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: 100 million cells were seeded in 15 cm dishes and the protein-DNA contacts were cross-linked using 1% (v/v) formaldehyde (Polysciences, 04018-1) for 10 min at room temperature with shaking ...
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bioRxiv - Immunology 2020Quote: ... BioInvent n-CoDeR® scFv were selected using biotinylated in-house produced recombinant LILRB3-hFc recombinant fusion proteins (captured with streptavidin-coated Dynabeads®) with or without competition or LILRB1-hFc coated to etched polystyrene balls (Polysciences, US) or plastic immunotubes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...