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Citations for Polysciences :
51 - 100 of 229 citations for CTLA 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Cell Biology 2019Quote: ... fixed for 15 min in 4% formaldehyde (Polysciences), washed 3 times with PBS and incubated 10 min with 15mM Glycine ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted in Mowiol 4-88 (Polysciences) and imaged on a Zeiss confocal microscope (LSM 880) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 37°C and permeabilized in ice-cold 90% methanol (Merck ...
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bioRxiv - Developmental Biology 2019Quote: ... After fixation with 4% PFA (Polysciences, Inc., 18814) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected midguts were fixed in 4% Formaldehyde (Polysciences) in 1x PBS for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% methanol-free formaldehyde (Polysciences) and 0.1 M potassium phosphate buffer (pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed 3 × 30 mins in PBS and then immersed through a gradient of sucrose solutions from 10%-50% at 4°C for > 4 hrs each before being embedded in cryomolds (Polysciences 18646A) using OCT (Sakura Finetek ...
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bioRxiv - Biophysics 2019Quote: ... the samples were fixed with 4% paraformaldehyde (PFA; Polysciences) in 1 x PBS for 10 minutes at 25 °C and subsequently rinsed with 1 x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... the COs were fixed with 4% paraformaldehyde (PFA; Polysciences) for 30 min at room temperature and subsequently embedded in cryogel (Tissue-Tek ® O.C.T ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with 4% EM grade Formaldehyde (Polysciences) for 5 minutes at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Genetics 2019Quote: ... The samples were fixed in either 4% formaldehyde (Polysciences) for 30 minutes or in Bouin’s fixative (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) for 10 min on ice before and then incubated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% formalin (Polysciences, 04018-1), permeabilized with 0.3% Triton X-100 in 1X PBS for 20 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were fixed in 4% glutaraldehyde (Polysciences; Cat#18428-5) and 2% acrolein (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... followed by fixation using 4% paraformaldehyde (18814-20, Polysciences Inc.) in 1X PBS for 20 min ...
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bioRxiv - Genetics 2019Quote: ... The surface attached cells were fixed in 4% formaldehyde (Polysciences) for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in PBS with 4% formaldehyde (Polysciences, Inc.) and 0.02% glutaraldehyde for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... 1.5 mg plasmids and 4 mg inear polyethylenimines (PEI) (Polysciences) were pre-incubated in 50 ml fresh medium for 30 min before adding into one litre cells at density of 3.0 × 106 cells ml−1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were embedded in JB-4 Plus plastic resin (Polysciences) and 5-10 μm sections were cut using a Leica RM 2155 microtome ...
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bioRxiv - Biochemistry 2022Quote: ... dissected midguts were immediately placed in fixative (4% formaldehyde (Polysciences), 0.2% saponin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed by adding 100 μl 4% paraformaldehyde (Polysciences) for 15 min ...
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bioRxiv - Systems Biology 2023Quote: ... transferred to a pre-fix solution containing 4% formaldehyde (PolySciences) in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS and stained twice with a 4% hydochloric acid and 4% potassium ferrocyanide (1:1 v/v) solution of for 25 minutes (Polysciences Prussian Blue stain kit, PA USA). After washing with PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescent images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were mounted with Mowiol 4–88 antifade medium (Polysciences Europe). Fixed neurons were imaged on a confocal laser-scanning microscope Leica TCS SP8 with identical settings used for all samples ...
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bioRxiv - Neuroscience 2020Quote: ... brain organoids were placed directly in 4% methanol-free formaldehyde (Polysciences) diluted in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). To block unspecific binding sites the fixed cells were incubated with blocking buffer containing 10% normal goat or donkey serum ...
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bioRxiv - Cell Biology 2021Quote: ... eyes were embedded in glycol methacrylate A resin (JB-4, Polysciences) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and infiltrated in glycol methacrylate resin (JB-4 resin; Polysciences, Inc). Serial parasagittal sections ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently fixed on the plate in 4% formaldehyde (Polysciences, Inc) for 30-40 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescence images were captured using a Nikon Eclipse NiE (40 ×/0.75 Plan Apo Nikon objective ...
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bioRxiv - Developmental Biology 2021Quote: ... ovaries were fixed in 4% formaldehyde (Polysciences, Inc., Warrington, PA, USA) in 0.1 M potassium phosphate buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... The tissue was fixed with 4% paraformaldehyde (Polysciences, Cat# 18814-20) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: Cells were fixed with 4% paraformaldehyde (PFA) (Polysciences, Warrington, United States). Unspecific binding sites were blocked by incubation with blocking buffer ...
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bioRxiv - Biophysics 2022Quote: ... Fixed cells were then mounted with Mowiol 4-88 (Polysciences, Inc.) onto glass slides and kept at 4 °C until imaging.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4-88 (Polysciences). Epifluorescence images were captured as z-projections using a Nikon A1R confocal microscope and analysed by the NIS-Elements software.
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bioRxiv - Neuroscience 2023Quote: ... Skin biopsies were fixed in 4% paraformaldehyde (PFA, Polysciences Europe GmbH) at 4°C overnight ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (w/vol) Mowiol 4-88 (Polysciences). Fluorescence images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Neuroscience 2024Quote: ... embryos or larvae were treated with 4% formaldehyde (Polysciences, Warrington, USA) in PBST at 4 °C over night and mounted in 1.5 % low melting point agarose (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were mounted using 10% (wt/vol) Mowiol 4–88 (Polysciences). Epifluorescent images were captured using a Nikon Eclipse NiE (40×/0.75 Plan Fluor Nikon objective ...
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bioRxiv - Developmental Biology 2024Quote: Preimplantation embryos (E2.5-E4.75) were fixed with 4% formaldehyde (Polysciences 04018) for 10 min ...
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bioRxiv - Neuroscience 2024Quote: Cells were fixed in 4% paraformaldehyde (PFA) (Polysciences, Warrington, FL, USA) for 10 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed in 4% methanol free PFA (Polysciences Cat# 040181) for 3 hrs at room temperature (RT ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were transfected with plasmids encoding ACE2 orthologs or human DPP4 using PEI 40K (Polysciences) according to manufacturer’s instructions ...