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Citations for Polysciences :
351 - 400 of 580 citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... mixed with the transfection reagent polyethylenimine hydrochloride (PEI Max, 24765-1, Polysciences, Inc., Warrington, PA, USA) at a 1:2 ratio (W/V) ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Cancer Biology 2019Quote: Retrovirus was produced in Plat-E cells by transient transfection with PEI (Polysciences; Cat#23966-1) according to standard protocols ...
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bioRxiv - Biochemistry 2022Quote: ... 30,000 cells per 48 well were plated and transfected with 1 μl PEI (Polysciences, PEI 25K), 75 ng of promoter reporter plasmids (3xMBS-pGL4.10 or pGl4.10 empty vector ...
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bioRxiv - Biophysics 2020Quote: ... 400 μg of plasmid was premixed with 1.2 mL of 1 mg/mL PEI MAX (Polysciences) in 15 mL of FreeStyle 293 expression medium ...
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bioRxiv - Neuroscience 2019Quote: ... DNA was mixed with 10 µl PEI (polyethylenimine, 1 mg/ml, Polysciences Europe GmbH, Eppelheim, Germany) in 500 µl MEM without supplements ...
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bioRxiv - Microbiology 2020Quote: ... Each expression vector was transiently transfected into Expi293 cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Physiology 2020Quote: ... animals were post-fixed with 1% aqueous solution of osmium tetroxide (Polysciences Europe GmbH 0972B-5) and 1.5% potassium ferrocyanide (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... These samples were washed in phosphate buffer and post-fixed in 1% osmium tetroxide (Polysciences Inc.) for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Bioengineering 2021Quote: ... 1 mg/mL plasmid DNA was mixed with 2 mg/mL linear 25 kDa polyethyleneimine (Polysciences) and transfected in OptiPRO SFM medium (4% final volume) ...
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bioRxiv - Cancer Biology 2022Quote: ... The transfection was performed with polyethylenimine (PEI MAX Transfection Grade Linear Polyethylenimine Hydrochloride, 24765-1, Polysciences) as described previously74 ...
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bioRxiv - Microbiology 2022Quote: ... The cells were trypsinized and fixed freshly prepared mixture of 1% glutaraldehyde (Polysciences Inc, Warrington, PA) and 1% osmium tetroxide (Polysciences Inc. ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in phosphate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc., Warrington, PA) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... At least 1×106 cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently incubated to 4 °C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Biophysics 2022Quote: ... the excess solvent was blotted and 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was added on the grid ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed at intervals post infection in freshly prepared mixture of 1% glutaraldehyde (Polysciences Inc.) and 1% osmium tetroxide (Polysciences Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... encoding rep and cap sequences together with transfection reagent polyethylenimine-Max (PEI-Max; Polysciences, #24765-1) at a ratio of plasmid DNA:PEI of 3:1 ...
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bioRxiv - Neuroscience 2024Quote: ... and frozen in a mixture of dry ice and ethanol in embedding molds (Polysciences 18646A-1) and left at -80 °C for up to one year ...
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bioRxiv - Biophysics 2019Quote: ... 1 μL of the PB including 3% fluorescent microbeads (ex:441, em:486, 1.0 μm, Polysciences, Inc.) was spread at the same time with the KI cells ...
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bioRxiv - Immunology 2021Quote: ... DNA transfections were performed using polyethylenimine (PEI) (1 mg/mL, linear, 25 kDa; Polysciences, Warrington, FL, USA) at a 3:1 ratio of PEI to DNA (w/w ...
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bioRxiv - Neuroscience 2019Quote: ... We then sterilized 1- and 6-µm diameter polystyrene microbeads (Polysciences, Cat#: 07310-15 and 07312-5) as noted in Cone et al. ...
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bioRxiv - Neuroscience 2021Quote: ... the tissues were rinsed twice in propylene oxide for 30 min each (Polysciences, Inc., cat# 00236–1). This was followed by incubation on a mixer at room temperature overnight in an approximately 1 mL mixture of 1 part propylene oxide and 1 part Polybed resin solution (Poly/Bed® 812 Embedding Media ...
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bioRxiv - Neuroscience 2021Quote: ... mixed with the transfection reagent polyethylenimine (PEI) hydrochloride (PEI Max, 24765-1, Polysciences, Inc., Warrington, PA, USA) at a 1:2 ratio (W/V) ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was added at a final concentration of 0.675 μg/mio cells and polyethylenimine (Polysciences, #23966-1) was added to a final concentration of 0.01 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were incubated using 1 mg/mL of Polyethylenimine (PEI, Linear, MW 25000, Polysciences, PA, USA) at a ratio of 1:6 (DNA:PEI) ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and mixed with 20 μl IMDM medium containing 1.2 μl of 1 mg/mL polyethylenimine (PEI, Polysciences). Following a 20 min incubation step at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed in phosphate buffer and post-fixed in 1% osmium tetroxide (Polysciences Inc., Warrington, PA) for 1 hr ...
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bioRxiv - Biophysics 2022Quote: ... The transfection solution was prepared by combining 5 μL of 1 mg/ml polyethylenimine Max solution (Polysciences) and a plasmid mixture consisting of 200 ng ssHA-FLAG-NTS1 and 500 ng alkaline phosphatase (AP)-tagged TGF-α (AP-TGFα ...
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bioRxiv - Bioengineering 2020Quote: ... After washing with acetic acid (1%, Fluka) samples were incubated with safranin O (0.1%, 15 min, Polysciences) to stain proteoglycans in red ...
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bioRxiv - Microbiology 2021Quote: ... The re-circularized genome was transfected into 293TT cells with PEI max transfection reagent (Polysciences, 24765-1). Progeny viral particles were purified on a linear cesium chloride gradient and titered as described previously (27 ...
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bioRxiv - Immunology 2020Quote: Peeled dermis and epidermis sheets were cut to 1 cm2 and fixed in 2% formaldehyde (Polysciences, 18814) and 30% sucrose in PBS for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The entire intestine was dissected out and fixed in 1× PBS plus 8% EM-grade paraformaldehyde (Polysciences) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were incubated in 50:50 mixture of methanol and JB4 resin (Polysciences, 00226-1, GMBH, Germany) overnight ...
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bioRxiv - Neuroscience 2022Quote: To identify sensory neurons innervating the knee joint retrograde labeling with Fast Blue (FB, #17740-1, Polysciences) was performed ...
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bioRxiv - Microbiology 2023Quote: ... and 2 μg plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... were purchased from Sigma Aldrich while PSS of Mw = 1 000 (PSS_5) and Mw = 8 890 (Mw/Mn = 1.09) (PSS_43) were bought from Polysciences and Scientific Polymer Products Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 500 ng plasmids expressing parental S or its derivatives using PEI Max (Polysciences, Cat# 24765-1) or TransIT-293 (Takara ...
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bioRxiv - Microbiology 2024Quote: HIV-1 Gag-iFRET plasmid DNA was transfected into HEK293T cells using a PEI transfection method (Polysciences) and into Jurkat cells using Lipofectamine LTX transfection reagent (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected with 1 μg/ml DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences). Conditioned medium containing the secreted recombinant protein was collected 4 days post transfection and filtered ...
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bioRxiv - Bioengineering 2024Quote: ... 150,000 cells/mL with 1 v/V% 500 nm fluorescent beads (Floresbrite YG carboxylate beads, Polysciences, Inc.) were encapsulated within each hydrogel on a Nunc glass bottom dish (Nunc Glass ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293FT cells were transfected with the lentiviral vector pCLTs together with the packaging plasmids pCMV-VSVG-RSV-Rev (addgene) and pCAG-HIVgp (addgene) at a 1:0.5:0.5 ratio using PEI Max reagent (Polysciences) according to the Manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells transfected with 1 µg of plasmids containing a gene of interest (Table 4) using polyethylenimine (PEI, Polysciences). 4 hours post transfection ...
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bioRxiv - Developmental Biology 2021Quote: For sectioning embryos in 100% methanol were mounted in JB4 plastic sectioning medium (Polysciences cat. no 00226-1). Dehydrated samples were infiltrated with 10 ml JB4 solution A plus catalyst for two days ...
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bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed for 1 h at 4°C in 4% paraformaldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Biophysics 2021Quote: ... cells were transfected using Env-deficient HIV-1 backbone plasmid pSG3ΔEnv (NIH ARRRP) and 25 kDa PEI (Polysciences) as a transfection reagent ...