Labshake search
Citations for Polysciences :
501 - 550 of 750 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Transfection solution was prepared by combining 20 μL (per dish, hereafter) of polyethylenimine solution (Polysciences; 1 mg/mL) and a plasmid mixture consisting of 500 ng Lg-βarr1 or Lg-βarr2 and 1μg μOR-Sm in 1 mL of Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... GST-ATG9 (828-839)-ATG101 were co-transfected with ATG13 (1-197) using the polyethylenimine (PEI)-MAX (Polysciences) transfection system ...
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bioRxiv - Cancer Biology 2022Quote: ... and 12 μg of pLKO-shRNA-puro using 63 μL of polyethylenimine (PEI – 1 μg/μL; Polysciences - 24765) mixed in 750 μL of 10 mM HEPES pH 7.4 and 150 mM NaCl in water ...
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bioRxiv - Physiology 2022Quote: ... 16 μg AAV-U6-sgRNA-TBG-Cre plasmid and 144 μl of 1 mg/ml Polyetherimide (PEI) (Polysciences) was mixed in 9 ml OptiMEM (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were transfected using a polyethylenimine (PEI) reagent (2.5 µl of 1 mg/ml per well hereafter; Polysciences) with these UTS2R plasmids (100 ng per well hereafter) ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed in sodium cacodylate buffer at room temperature and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with 67.5 μg of polyethyleneimine (PEI) Max 40,000 (Polysciences, Inc, Cat. No. 24765-1) in complex with 3.15 μg of plasmid encoding a spike variant ...
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bioRxiv - Biophysics 2023Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 121.6 μL of 1 mg/mL of linear Polyethylenimine (PEI; MW 25,000; Polysciences) and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... 1.2 μg of DNA was mixed with 2.4 μL of 1 mg/mL polyethylenimine (linear PEI, MW 25,000, Polysciences) in one tenth of the final culture volume of medium ...
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bioRxiv - Biophysics 2024Quote: ... Cells were transfected using a polyethylenimine (PEI) agent (10 μl of 1 mg/ml per dish hereafter; Polysciences) with pCAG plasmids encoding human A1R ...
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bioRxiv - Developmental Biology 2021Quote: ... were stained with 2% uranyl acetate (Polysciences; Cat#6159-44-0) and lead citrate (Polysciences ...
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bioRxiv - Molecular Biology 2019Quote: ... Polyethylenimine (PEI) 25kD Linear Form was obtained from Polysciences (#23966-2). All other materials were of reagent grade.
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bioRxiv - Cancer Biology 2022Quote: ... and pLP/VSVG (2 µg) (clontech) using Polyethylenimine (PEI) Max (Polysciences). Virus containing supernatant was collected 48 h after transfection and cleared through a 0.45-µm PDF filter ...
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bioRxiv - Neuroscience 2021Quote: ... or 5 µl of 2% Fast-Blue (Polysciences, Warrington, PA, USA). Tracer leakage was prevented by sealing the reservoir around the nerve with Vaseline (Vaselinum album ...
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bioRxiv - Neuroscience 2020Quote: ... and 48 μl of 2 mg/mL polyethyleneimine (PEI; Polysciences, 24765) were mixed and transfected into Lenti-X 293T (Clontech ...
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bioRxiv - Physiology 2021Quote: ... Cells were transfected with Polyethylenimine-PEI Transfection Reagent (Polysciences, 23966-2) carrying the LC3-eGFP plasmid (60 ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfection of packaging cells was performed using polyethyleneimine (Polysciences, 23966-2) by mixing with DNA in a 3:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: NGN2-iN lentiviruses were produced by polyethylenimine (Polysciences, PEI, 23966-2,) in HEK 293T cells (ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... using Polyethylenimine according to the manufacturer’s instructions (PEI, Polysciences #24765-2).
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bioRxiv - Cancer Biology 2023Quote: ... were transfected into HEK293FT cells using 2 μg of Polyethylenimine (Polysciences). Lentiviral particle containing media was filtered using 0.45μm cellulose acetate syringe filter and used for transduction ...
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bioRxiv - Microbiology 2020Quote: ... The fixed suspension of bacteria was washed in sodium cacodylate buffer and postfixed in 1% osmium tetroxide (Polysciences Inc.) for 1 h ...
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Novel amiloride derivatives that inhibit bacterial motility across multiple strains and stator typesbioRxiv - Microbiology 2021Quote: ... and incubated for 10 mins and washed with motility buffer and flushed with 1 μm polystyrene bead (Polysciences, Inc) solution ...
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bioRxiv - Immunology 2021Quote: ... 480µg of each plasmid was co-transfected with 120µg of BirA (Howarth et al., 2008) and 12mg PEI-Max (1 mg/mL solution, Polysciences) in the presence of 200 µM biotin (final concentration) ...
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bioRxiv - Neuroscience 2021Quote: ... Day 1 adults were placed on a 10% agarose pad and immobilized with a slurry of polystyrene beads (Polysciences). A 1KHz train of 100 fs pulses (15-30 nJ/pulse ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Immunology 2021Quote: ... pSIREN- or pMXs-based plasmids were co-transfected together with pVSVG into Plat-E cells (kindly provided by T. Kitamura, University of Tokyo) using PEI Max (Mw 40,000, 24765-1; Polysciences). The virus-containing supernatant was harvested 2 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and sorting efficiency was verified manually by sorting a specified number of 1-μm yellow-green microspheres (Polysciences, USA) onto a glass slide and counting the beads under an epifluorescence microscope ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear, MW 25,000, 23966-1-A, Polysciences) transfection method (45) ...
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bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
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Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2022Quote: ... expi293F cells were transfected with antibody expression plasmids at a density of 2.5 million cells per/ml using 1 mg/ml PEI-Max transfection reagent (Polysciences). Supernatants were harvested 4-5 days post-transfection and tested for their SARS-CoV-2 RBD binding potential by enzyme-linked immunosorbent assay (ELISA) ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Microbiology 2023Quote: HIV-1 viruses were generated by transfection of proviral DNA into HEK293T cells via polyethylenimine (PEI, Polysciences, Cat. #23966) transfection protocol ...
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bioRxiv - Biophysics 2023Quote: ... The cells in each well were transiently transfected with 1 μg DNA plasmid using 3μg linear polyethyleneimine (PEI, MW 25000, Polysciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Imaging was carried out by placing adult worms in 1.5 μl of M9 mixed with 1.5 μl of 1 μm polysterene microspheres (Polysciences Inc.) on a coverslip which was mounted carefully onto a 5% agarose pad.
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Fluoresbrite® yellow-green polystyrene-based microspheres with a diameter of 1 µm (Cat# 17154-10) purchased from Polysciences as 2.5% w/v in a sterile aqueous suspension without sodium azide ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... or with GFP-tau and vectors pCDNA3 overexpressing Cdk5 and p25 (1:0.85:0.15) using linear Polyethylenimine (PEI; Polysciences, Inc.). Cells were harvested 48 hours after transfection by centrifugation and flash-frozen ...
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bioRxiv - Cell Biology 2022Quote: ... 2-3μl of 1.25% (w/v) polystyrene microspheres (Polybead Microspheres 0.05μm, Polysciences) and 0.2mM levamisole (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Cells were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer pH 7.2 for 1 h at room temperature and subsequently transferred to 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... samples were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100mM PIPES/0.5mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... were produced as previously described using a PEI (polyethylenimine, Polysciences, 24765-2) transfection protocol followed by iodixanol gradient purification ...
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bioRxiv - Molecular Biology 2019Quote: Overexpression of vectors was performed using Polyethylenimine MAX 4000 (Polysciences, 24885-2) with a DNA:PEI ratio of 1:10 ...