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Citations for Polysciences :
51 - 100 of 971 citations for 7 cyano 1 2 3 4 tetrahydroisoquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...
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bioRxiv - Neuroscience 2023Quote: Cells for ICC were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biochemistry 2024Quote: ... in a 6:4:1 ratio using PEI-max (Polysciences #24765-100). Virus-containing medium was harvested two days post-transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl polyethylenimine (PEI) 25K (1mg/ml; Polysciences, 23966-1) per μg of plasmid DNA were mixed with pre-warmed Opti-MEM media (ThermoFisher Scientific ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 600 ng of PsPax2 (Addgene # 12260) ...
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bioRxiv - Microbiology 2023Quote: ... Biofilm competitions utilized 7 mm polystyrene beads (Polysciences, Inc, Warrington, PA) as a surface for cell attachment ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biophysics 2023Quote: ... spin coated in 1% (w/w) PVA (Mowiol 4-88, #17951, Polysciences Inc.) in nanopure water on a coverslip.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... 1 mg of plasmid was mixed with 4 mg of polyethyleneimine (PEI; PolySciences Inc.) in Opti-MEM cell culture medium (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... 1 uL (or 1.5 uL for target-binding experiments) of 1 mg/mL PEI (Polysciences, 24765-2) dissolved in water was added to DNA in media and vortexed for 10 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 1 μg ml-1 DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences), pre-incubated for 15 min in Optimem medium (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Biophysics 2022Quote: ... for 1 hour and finally mounted on glass slides with Mowiol 4-88 (Polysciences, Inc.) and kept at 4°C overnight.
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bioRxiv - Neuroscience 2021Quote: ... neurons were stained with 1 mL of 2% uranyl acetate (Polysciences #21447-25) in water for 30 min at room temperature and then briefly washed with 50% ethanol (Pharmco #111000200 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg/μL PEI (Polyethylenimine HCl MAX Linear MW 40000 (PolySciences, 24765–2)) and 1 μg/ μL DNA (3:1 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected using polyethylenimine (PEI) (Polysciences, 23966-2, 1 mg/mL) and 3rd generation lentivirus packaging vectors (pMDLg ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of 1 mg/ml linear Polyethylenimine (PEI; Polysciences, Inc, 24765-2) and 1-2 µg of each plasmid ...
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bioRxiv - Biophysics 2019Quote: ... 1 μL of the PB including 3% fluorescent microbeads (ex:441, em:486, 1.0 μm, Polysciences, Inc.) was spread at the same time with the KI cells ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... 4 ml (from 1 mg/ml stock) of linear polyethyleneimine (PEI) MAX (Mw 40,000; Polysciences Inc.) was diluted in the same medium to a volume of 30 ml in a separate tube and mixed ...
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bioRxiv - Neuroscience 2019Quote: ... and 1 µm (2 µL at 1.5×107 beads/µL; Polysciences, Cat#: 07310-15) polystyrene microbeads through a 50-100 µm cannula in the cornea formed by a beveled glass micropipette connected by polyethylene tubing to a Hamilton syringe (Hamilton Company Reno ...
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bioRxiv - Molecular Biology 2022Quote: A stock solution of 1 mg / ml linear Polyethylenimine (PEI; Polysciences, Inc 24765-2) was prepared in a buffer containing 25 mM Hepes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were picked up onto 1×2 mm formvar coated copper slot grids (Polysciences), and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Physiology 2020Quote: ... were transiently co-transfected with either A1R-Nluc or A3R-Nluc and β-arrestin2-YFP (total 2 μg, at a 1:6 ratio) using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...