Labshake search
Citations for Polysciences :
351 - 400 of 1055 citations for 7 chloro 3' 4 6 trimethoxy 5' methylspiro 1 benzofuran 2 6' cyclohex 2 ene 1' 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... The transfection was performed with a 1:1 (w:w) HC/LC ratio using a 1:2.5 ratio with 1 mg/l PEImax (Polysciences) in 200 μl Opti-MEM ...
-
bioRxiv - Microbiology 2023Quote: ... and pHelper (CellBio Labs) at a 1:1:1 ratio using polyethyleneimine (PolySciences). Supernatant was collected 72hrs post-transfection ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected with BirAGFP-KIF13B Tail 2 or Tail 3 constructs (designated C2 and C3, respectively, in (Serra-Marques et al., 2020)) using Polyethylenimine (PEI; Mw 2500; Polysciences) at a 3:1 PEI:DNA ratio (w/w) ...
-
bioRxiv - Cell Biology 2019Quote: ... samples were washed 3 times for 30 mins with PBS then incubated through a cryoprotective series of sucrose solutions of increasing concentration from 10-50% for ≥3 hours each before embedding into OCT compound (Tissue Tek) in disposable cryomolds (Polysciences) and frozen in an isopentane bath cooled by liquid nitrogen ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
-
bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
-
bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... For formation of DNA:PEI complexes 1 μg DNA/mL transfection volume and 5 μg of 40 kDa PEI (Polysciences) were first diluted separately in 5% transfection volume in supplemented F17 media ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
-
bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed 4 x 5 min in PBS and mounted with Aqua-Poly/mount (Polysciences, 18606). STED images were acquired by sequential acquisition of each channel ...
-
bioRxiv - Biochemistry 2023Quote: ... and pCAG ATG14 or mCherry-ATG14 plasmids were co-transfected at a 1:1:1:1 mass ratio using the polyethelenimine (PEI) (Polysciences) transfection method ...
-
bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then washed and fixed with 2% ultra-pure formaldehyde (Polysciences, Warrington, PA) for 20 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... 1.5 μl retrograde tracer Fast Blue (2% w/v in 0.9% saline; Polysciences, Germany) was injected into the operated stifle joints 7 days prior to mouse sacrifice to label knee innervating neurons.
-
bioRxiv - Biophysics 2021Quote: ... The water surrounding the spheres was seeded with 2 µm polystyrene spheres (Polysciences 19814). PIV analysis was performed using PIVlab [53] ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with polyethylenimine (PEI; linear, MW 25,000; Polysciences, cat. No. 23966-2) at a 2:1 ratio (PEI:DNA ...
-
bioRxiv - Immunology 2021Quote: ... Small-scale vector preparations were generated by polyethylenimine or PEI (Polysciences, Cat #24765-2) triple transfection of AC1 or AC3 ITR-flanked transgene ...
-
bioRxiv - Biochemistry 2022Quote: ... An aqueous 10µg/ml stock of linear 25kDa PEI (Polysciences, catalogue number 23966-2) was used for the transfection ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 19.5 μg of psPAX2 were transfected using polyethylenimine (1μg/μl, Polysciences # 23966-2) into 293T cells plated in 6-well plates ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were infiltrated with 1:1 Polybed812 (Polysciences) resin:acetone for 24 hours ...
-
bioRxiv - Cell Biology 2022Quote: ... worms were mounted on 8–10% agarose pads in 3 μL of a 0.5 μM polystyrene bead slurry (Polysciences, Warrington, PA) in PBS pH 7.4 (Kim et al. ...
-
bioRxiv - Developmental Biology 2020Quote: ... 0.1% Triton X-100, 2.5% DABCO) (Hama et al., 2011) for at least 3 days and mounted in Aqua-Polymount (Polysciences Inc. 18606). Whole-mount colorimetric in situ hybridizations and live worms were imaged on a Leica M165F ...
-
bioRxiv - Molecular Biology 2021Quote: ... OSCs were harvested and 500.000 OSCs were washed three times and coupled to 10 µl of activated Concanavalin A-coated magnetic beads (Polysciences, #86057-3) per sample ...
-
bioRxiv - Immunology 2023Quote: ... 0.5 mM spermidine in 1X protease inhibitor buffer) and incubated with activated concanavalin A (ConA) coated magnetic beads (Polysciences - 86057-3) in PCR strip tubes at room temperature for 10 minutes ...
-
bioRxiv - Developmental Biology 2020Quote: ... High resolution episcopic microscopy (HREM) was performed as previously described97 using an JB-4 embedding kit (00226-1, Polysciences GMBH, Germany). μMRI and HREM data were analysed using OsiriX MD DICOM viewer version 9.0.2 (Pixmeo) ...
-
bioRxiv - Cell Biology 2022Quote: ... were transiently co-transfected with 4xPylT/PylRS(Y306A, Y384F) and 4xPylT/EMC3(Amber[TAG])-GFP plasmids at a ratio of 4:1 using PEI “MAX” (Polysciences, USA). The cells were grown in the presence of 0.5 mM AbK (Iris Biotech ...
-
bioRxiv - Microbiology 2023Quote: ... then a 1:1 solution of ethanol:Hexamethyldisiloxazne (HMDS) (Polysciences) followed by pure HMDS ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 1 mg/mL polyethylenimine (PEI) (Polysciences, 23966-1) in a 4:1 ratio of DNA (μg):PEI (μg) ...
-
bioRxiv - Microbiology 2021Quote: ... highly synchronized infected RBCs were fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
-
bioRxiv - Bioengineering 2023Quote: ... and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI, Cat# 23966, Polysciences). The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol ...
-
bioRxiv - Cell Biology 2023Quote: Transfections were done transiently using PEI transfection reagent (1 mg ml-1 stock, Polysciences, 24765-1), 4 µg of transfection reagent per 1 µg of plasmid ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 15min at RT after which they were washed once for 15min in PBST and 3×15min in PBS before mounting with Aqua-Poly/Mount (Polysciences, #07918606-20). All experiments were performed with wild-type mice from the NMRI strain and carried out upon approval and following the guidelines of the ethics committee of IGC.
-
bioRxiv - Microbiology 2019Quote: ... were co-transfected with 3 µg Gibson Assembly product DNA plus 330 ng pHEF-VSV-G using polyethylenimine (Polysciences, Inc., Warrington, PA) at a ratio of 1 µg total DNA to 4 µg polyethylenimine in 800 µl of 150 mM NaCl [72] ...
-
bioRxiv - Physiology 2024Quote: ... The balloon was inflated after the first diagonal branch of the LAD in each animal and 3 ml polystyrene microspheres (Polybead, 90 μm, Polysciences, Warrington, PA) were slowly injected via the lumen of the angioplasty catheter ...
-
bioRxiv - Neuroscience 2020Quote: ... UK) and the left tibia was injected with 5 μl of 4% Fast Blue neuronal tracer (Polysciences Inc., Germany). After a 7-day recovery period ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV7A2 and AAV9A2 caps were used in this study) and adenoviral helper plasmid at a 1:1:1 molar ratio using a polyethylenimine (PEI MAX; Polysciences, Warrington, USA, cat.no. 24765-1) as the transfection reagent ...
-
bioRxiv - Synthetic Biology 2019Quote: ... A master stock of PEI was made by dissolving linear PEI (Polysciences Cat. No. 23966-2) in deionized water at a concentration of 0.323g/L ...
-
bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
-
bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
-
bioRxiv - Cell Biology 2022Quote: ... A total of 1 µg per one million cells of the plasmids containing CMV promoter and 3×FLAG-tagged geneswere mixed with a three-fold excess (w/w) of polyethylenimine MAX 40 kDa (Polysciences, Warrington, PA, USA) in 4 ml OptiPro (Invitrogen) ...
-
bioRxiv - Biochemistry 2021Quote: ... the samples were incubated for 3 h at RT in the mixture of EtOH and LR White resin (Polysciences, Inc., Warrington, PA, USA) in the following proportions ...
-
bioRxiv - Microbiology 2022Quote: ... followed by a 1:2 mixture of propylene oxide for and Polybed 812 epoxy resin for 3 hours and transferred to 100% resin to embed overnight (Polysciences, Inc.,Warrington, PA). Ultrathin sections (70-80 nm ...
-
bioRxiv - Microbiology 2020Quote: ... we introduced 1 mL of 1×109 / mL 1 µm diameter YG carboxylate fluorescent microspheres (Polysciences, Warrington, PA) to the overlying water of a LSSM sediment core ...
-
bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
-
bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
-
bioRxiv - Cell Biology 2020Quote: ... 900 μL 1 mg/mL PEI MAX 40k (Polysciences, 24765-1) and 300 μg DNA ...