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Citations for Polysciences :
251 - 300 of 1011 citations for 7 Oxa 1 thia 3 azaspiro 4.4 non 2 ene 4 6 dione 2 amino 8 8 dimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cells were harvested by centrifugation and fixed by the addition 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc.) in 100 mM sodium cacodylate buffer (pH 7.2 ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed with fresh 3% paraformaldehyde at 4°C for 10 mins and smeared on poly-L-lysine pre-coated slides (Polysciences, Inc., USA). Cells were washed once with 1× PBS and incubated with 100 times dilution of BODIPY® FL vancomycin (Van-FL ...
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bioRxiv - Neuroscience 2023Quote: Cells for ICC were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Neuroscience 2019Quote: ... We then sterilized 1- and 6-µm diameter polystyrene microbeads (Polysciences, Cat#: 07310-15 and 07312-5) as noted in Cone et al. ...
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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bioRxiv - Cell Biology 2020Quote: RPE1 cells were transfected with PX458 vector with specific BBS sgRNA using polyethylenimine (Polysciences, Inc., 23966-2). After 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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bioRxiv - Bioengineering 2023Quote: ... Transient transfection mixtures were created by mixing 2 µg of plasmid mixture with polyethyleneimine (PEI-MAX, Polysciences) at 4 μg of polyethyleneimine per microgram of DNA ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 600 ng of PsPax2 (Addgene # 12260) ...
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bioRxiv - Microbiology 2023Quote: ... Biofilm competitions utilized 7 mm polystyrene beads (Polysciences, Inc, Warrington, PA) as a surface for cell attachment ...
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bioRxiv - Neuroscience 2021Quote: iPSC-derived cortical neurons were fixed with 4% formalin (Polysciences, cat.04018-1) for 15 mins ...
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bioRxiv - Biophysics 2023Quote: ... spin coated in 1% (w/w) PVA (Mowiol 4-88, #17951, Polysciences Inc.) in nanopure water on a coverslip.
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Cell Biology 2019Quote: ... and 500 µL of the melted agarose was supplemented with 15 µL of 2-µm polystyrene beads (Polysciences). Twenty µL each of the FP and agarose/beads solutions were mixed thoroughly ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were transfected with 2 µg total DNA and 7.35 µl polyethylenimine HCl MAX transfection reagent (Polysciences, Inc). Wells co-transfected with mClover3-TDPΔNLS and HSP104 variants received 1 µg of each plasmid ...
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bioRxiv - Microbiology 2021Quote: ... spores were harvested in KK2 buffer and fixed with 2% paraformaldehyde with 2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2020Quote: ... Live animals were immobilized with 0.01% tetramisole and 0.1% tricaine in M9 buffer and mounted on 2% agarose pads or with 0.05 μm Polybead microspheres (Polysciences) diluted 1:2 in water and mounted on 5% agarose pads ...
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bioRxiv - Developmental Biology 2019Quote: ... and triple transfected into 10 15-cm plates of HEK293T cells using PEI transfection reagent (Polysciences, 23966- 2). 60 h after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... 293T media was changed and the cells were transfected using 1mg/ml of polyethylenimine (PEI) (Polysciences, 23966-2).
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20U/ml) for 2 min at 35°C followed by 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% formaldehyde (Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and heparin (20 U/ml) for 2 min at 35°C followed by 0.15 M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences), 2% paraformaldehyde (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 4µg pMDG.2 plasmid and 4µg psPAX plasmid by using the transfection reagent Polyethylenimine (PEI) (Polysciences #23966-100) at a PEI:DNA ratio of 4:1 ...
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bioRxiv - Immunology 2022Quote: ... HEK293E cells were transfected at a density of 106 cells/mL using 0.5 mg/mL of pTT3-RBD-HISAVI DNA and 2 mg/mL filter sterilized pH 7.0 Polyethylenimine (PEI) MAX (40,000 MW, Polysciences). Six days later ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by 2 minutes incubation of the slices in 0.15M cacodylate buffer containing 2.5% glutaraldehyde (Polysciences). rinsed 5 × 2 min in chilled buffer ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Immunology 2022Quote: ... 1 mg of plasmid was mixed with 4 mg of polyethyleneimine (PEI; PolySciences Inc.) in Opti-MEM cell culture medium (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Cell Biology 2024Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in MilliQ water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Whole larva were then mounted between a 2% M9 agarose pad and coverslip in M9 containing 0.1μm polystyrene beads (Polysciences, Inc.) and 10 mM Levamisole to induce worm paralysis (Reich et al. ...
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bioRxiv - Immunology 2020Quote: Mice were given a single oral gavage of 2 × 1011 of Fluoresbrite Yellow Green labelled 200 nm microbeads (Polysciences, Hirschberg an der Bergstrasse ...
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bioRxiv - Genetics 2019Quote: ... a kind gift from the Nolan lab (https://web.stanford.edu/group/nolan/_OldWebsite/retroviral_systems/phx.html) with Polyethylenimine (PEI, Polysciences Cat. 23966-2). Viral supernatant was collected after 48 hours post transfection.
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bioRxiv - Genomics 2021Quote: ... and 2 μm-diameter fluorescent beads were added to each sample as an internal standard (Polysciences, Warminster, PA, USA). The samples were analyzed on a FACSCanto™ II Flow Cytometry Analyzer Systems (BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: Leishmania promastigotes were harvested in logarithmic growth phase and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM phosphate buffer ...
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bioRxiv - Immunology 2023Quote: ... luciferase encoding reporter plasmid (pTG126) and plasmid encoding Wuhan strain SARS-CoV-2 S using 24 μL polyethylenimine (Polysciences) in Optimem (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using PolyJet™ DNA In Vitro Transfection Reagent (Signagen, #SL100688) or PEI (Polysciences Inc, 23966-2) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... containing 10 µL of PEI reagent (2 mg/mL concentration of PEI MAX MW 40,000: Polysciences, Warrington, PA, USA) and then transfected into 293T cells grown to confluency in 6-well plates ...
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bioRxiv - Cell Biology 2023Quote: Cells grown on 3 mm aclar disks (carbon coated) were fixed using a mixture of 2% (v/v) formaldehyde and 0.2% (v/v) glutaraldehyde (Polysciences) in 0.1M phosphate buffer ...