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Citations for Polysciences :
351 - 400 of 900 citations for 7 METHYLIMIDAZO 1 2 B 1 2 4 TRIAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... transfection was carried out using 0.5 μg of plasmid DNA and 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Calculations of relative fluorescence per cell were done by normalizing red chlorophyll fluorescence per cell to 2 μm reference fluorescent beads (catalog no. 18604; Polysciences, Warrington, PA, USA) as previously described [72] ...
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bioRxiv - Microbiology 2021Quote: ... 5×105 cells per well in 6-well plates were transfected with 2 μg of plasmid using 8 μg of polyethylenimine (23966, Polysciences, Inc., Warrington, PA), which was reconstituted at 1 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... The transfection was performed using linear polyethyleneimine hydrochloride (40,000, PEI MAX, Polysciences, Cat# 49553-93-7). For each vector 5× 15cm dishes were transfected and subsequently AAV particles were purified from cell pellet and media using iodixanol gradient purification as previously reported79.
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bioRxiv - Biochemistry 2021Quote: ... and wildtype b0,+AT (the pEZT-C-TwinStrep-mCherry-OaSLC7A9 plasmid) were transfected into HeLa cells at 1:1 molar ratio by using PEI MAX (40,000 MW Linear; Polysciences, Inc.). The ratio of DNA ...
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... RIKEN BRC) [22] were mixed with 111 µl of 1 mg/ml PEI MAX® (pH7.5) (Cat. No. 24765-1, Polysciences) in Opti-MEM™ I Reduced Serum Medium (Cat ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Immunology 2021Quote: ... using PEI Max (1 mg/mL, Polysciences, Inc.) at a 3:1 ratio (PEI Max:DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... Six□micrograms of PEI-Max (Polysciences, #24765-1) were used for transfection ...
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bioRxiv - Microbiology 2021Quote: ... using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). To measure NS3/4A activity ...
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bioRxiv - Microbiology 2021Quote: ... 1 M anti-digoxygenin coated polystyrene beads (Polysciences) were conjugated to the peptide construct through λ-DNA of the peptide construct by incubation in a buffer containing 10 mM Tris HCl pH 7.5 and 150 mM NaCl for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). For the NKG2D blocking assay ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). OT-1 cells were analyzed by flow cytometry using a FACSCanto II cell analyzer (Becton Dickinson ...
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bioRxiv - Biophysics 2019Quote: ... 1 μM anti-digoxigenin coated polystyrene beads (Polysciences), diluted in a buffer containing 10 mM Tris HCl pH 7.5 and 50 mM NaCl were added to the imaging chamber and incubated for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl PEI-max (1 μg/μl; Polysciences) was mixed with DNA (1 μg/ml cells ...
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bioRxiv - Neuroscience 2023Quote: ... or GluN2B using PEI Max (Polysciences, 24765-1). 24 h after transfection ...
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bioRxiv - Microbiology 2023Quote: ... 500 µL PEI-max (1 µg/µL; Polysciences) was mixed with DNA (1 µg/mL cells ...
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bioRxiv - Immunology 2023Quote: ... 72 uL 1 mg/mL polyethylenamine (Polysciences 23966), and 600 uL DMEM ...
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bioRxiv - Immunology 2023Quote: ... using PEI Max (1 mg/mL, Polysciences, Inc.) at a 3:1 ratio (PEI Max:DNA) ...
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bioRxiv - Microbiology 2023Quote: ... for 1 min and Phosphotungstic acid (Polysciences, 25088D) for 10 mins with washes between each step ...
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bioRxiv - Developmental Biology 2024Quote: ... positioned in an embedding mold (18646A-1, Polysciences) containing 100% OCT ...
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bioRxiv - Cancer Biology 2019Quote: ... 7.5×106 HEK293T cells were seeded into 10 cm dishes and transfected with the vector of interest using PEI (Polysciences 23966-2, Warrington, PA) along with pCMV-VSV-G (Addgene #8454 ...
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bioRxiv - Microbiology 2020Quote: ... ANDV GPC or HTNV GPC (100 μg, in pCAGGS vector) and Tim-1 (6 μg) using PEI (3:1 PEI: DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Bioengineering 2021Quote: ... and packaging plasmids pCMV-dR8.91 (900 ng) and pCMV-VSV-G (100 ng) with 12 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences 24765-1) into HEK293T cells that had been grown to 60-80% confluence in 6-well plates ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Immunology 2020Quote: ... 1 milligram of plasmid DNA encoding HIV-1 Env per 1 liter of cells was diluted in DMEM and mixed with polyethyleneimine (PEI, Polysciences, Inc.). The DNA was allowed to complex with PEI for 25 min and then cells were added ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then transfected with 1 μg of JFH1-QL/GLuc2A in vitro transcribed RNA (1 μg) using polyethylenimine (Polysciences, Inc.). Supernatants were harvested and mixed with Renilla luciferase buffer and substrate ...
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bioRxiv - Microbiology 2024Quote: ... and NiV-M-GFP at a 9:1:5 ratio by using polyethylenimine (PEI) at 1 mg/ml (Polysciences, 23966-100). At 48hrs posttransfection ...
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bioRxiv - Neuroscience 2021Quote: ... COS-7 cells were transiently transfected with appropriate cDNA using PEI (Polyethylenimine, linear, MW 25000; Polysciences, Inc).
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bioRxiv - Biophysics 2021Quote: ... at a density of 5-7 million cells and transfected the next day with PEI (Polysciences, Germany) and 16:4:4:8 μg DNA encoding hASIC1a TAG variants ...
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bioRxiv - Neuroscience 2020Quote: ... fixed in 4% Formaldehyde (Polysciences 18814) / PEM buffer (0.1 M PIPES ...
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bioRxiv - Microbiology 2021Quote: ... were fixed with 4% formaldehyde (Polysciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2020Quote: ... fixed in 4% paraformaldehyde (Polysciences, #18814) and mounted in Prolong Diamond to preserve GFP signal were also prepared ...
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bioRxiv - Physiology 2021Quote: ... ATP [5mM] and 4% formaldehyde (Polysciences). After fixation ...
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bioRxiv - Microbiology 2022Quote: ... 200 μl of 4% PFA (Polysciences) was added to the upper chamber of the Transwells and incubated for 30 min ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...