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Citations for Polysciences :
251 - 300 of 1042 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Cell Biology 2020Quote: ... from Bangs Laboratories and Latex polymer 3-micron beads (17134-15) from Polysciences. Murine IFNγ (315-05 ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 15 µl of BioMag ConcanavalinA beads (Polysciences, #86057-3) in binding buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed 3 × 30 mins in PBS and then immersed through a gradient of sucrose solutions from 10%-50% at 4°C for > 4 hrs each before being embedded in cryomolds (Polysciences 18646A) using OCT (Sakura Finetek ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then rinsed 1 x 5 min with Tris buffer without milk powder and mounted using Aqua-Poly/Mount (Polysciences, Eppelheim, Germany). Antibodies were specific for peripheral myelin protein (PMP2 ...
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bioRxiv - Neuroscience 2023Quote: ... amphotericin B (0.5 mg ml−1) and small amounts of glass beads (5–15 μm in diameter; Polysciences, Inc., Warminster, PA, USA) (pH 7.3 ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Biophysics 2019Quote: ... the samples were fixed with 4% paraformaldehyde (PFA; Polysciences) in 1 x PBS for 10 minutes at 25 °C and subsequently rinsed with 1 x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... the COs were fixed with 4% paraformaldehyde (PFA; Polysciences) for 30 min at room temperature and subsequently embedded in cryogel (Tissue-Tek ® O.C.T ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with 4% EM grade Formaldehyde (Polysciences) for 5 minutes at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Genetics 2019Quote: ... The samples were fixed in either 4% formaldehyde (Polysciences) for 30 minutes or in Bouin’s fixative (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) for 10 min on ice before and then incubated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... linear mw 25,000 (Polysciences 23966-2). Cells were washed with DMEM or DMEM-F12 2-4 hours after transfection reagent was added (Longo et al. ...
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bioRxiv - Cell Biology 2021Quote: ... After fixation with 2% glutaraledhyde (Polysciences) in PBS (pH7.4) ...
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bioRxiv - Bioengineering 2021Quote: ... polystyrene latex microspheres (Polysciences, 18142-2) with our HELP gels ...
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bioRxiv - Bioengineering 2023Quote: ... 2-sulfoethyl methacrylate (PolySciences, Warminster, PA) was added to a final concentration of 5 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were bound to Concanavalin A beads (86057-3, Polysciences Inc), followed by permeabilization using a digitonin containing buffer ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were fixed in 3% glutaraldehyde in 0.1 ml PBS (Polysciences) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... 250.000 cells were captured with BioMagPlus Concanavalin A (Polysciences, 86057-3) and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4 ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 μl of BioMag Plus Concanavalin A beads (Polysciences # 86057-3) were washed twice in 500 μL of Binding Buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Immunology 2024Quote: ... 10uL/reaction of concanavalin A-coated beads (Cat #86057-3, Polysciences) were equilibrated with Binding Buffer (20mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Stable cell lines expressing N-terminal Flag tagged receptor constructs in pcDNA3.1 vector were generated by transfecting HEK-293 cells with 7 μg of plasmid DNA using polyethylenimine (PEI) (Polysciences, Cat. no. 19850), followed by selection using G418 (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... pCAG-HIVgp (5 μg) and pCMV-VSV-G-RSV-Rev (5 μg) (Riken BioResource Research Center) using PEImax solution (60 μL) (Polysciences) prepared in KnockoutDMEM/F-12 (500 μL ...
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bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
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bioRxiv - Biochemistry 2020Quote: ... 5 µm Polybead carboxylate microspheres (Polysciences, Warrington, PA) were covalently coupled with goat anti-human pAb using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/EDC chemistry (49) ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids and PEI (product #260008-5; Polysciences) were separately diluted in Opti-MEM1 (product #31985-047 ...
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bioRxiv - Cell Biology 2024Quote: ... Polystyrene microspheres (Polysciences #00876-15; 5-fold dilution) were used to immobilize late-L4 animals on 10% agarose pads ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Cell Biology 2022Quote: ... followed by fixation using 4% paraformaldehyde (18814-20, Polysciences Inc.) in 1X PBS for 20 min ...
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bioRxiv - Genetics 2019Quote: ... The surface attached cells were fixed in 4% formaldehyde (Polysciences) for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed in PBS with 4% formaldehyde (Polysciences, Inc.) and 0.02% glutaraldehyde for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... 1.5 mg plasmids and 4 mg inear polyethylenimines (PEI) (Polysciences) were pre-incubated in 50 ml fresh medium for 30 min before adding into one litre cells at density of 3.0 × 106 cells ml−1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Ovaries were embedded in JB-4 Plus plastic resin (Polysciences) and 5-10 μm sections were cut using a Leica RM 2155 microtome ...
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bioRxiv - Biochemistry 2022Quote: ... dissected midguts were immediately placed in fixative (4% formaldehyde (Polysciences), 0.2% saponin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed by adding 100 μl 4% paraformaldehyde (Polysciences) for 15 min ...
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bioRxiv - Systems Biology 2023Quote: ... transferred to a pre-fix solution containing 4% formaldehyde (PolySciences) in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...