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Citations for Polysciences :
351 - 400 of 777 citations for 6 Methyl 2 7 naphthyridin 1 2H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mt-GCaMP6f: AAV1) and transfer plasmids were mixed and diluted in saline before mixing with saline-diluted polyethylenimine (PEI, Polysciences #23966-2) and brief vortexing ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
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bioRxiv - Microbiology 2020Quote: ... and co-transfected with the two IgG plasmids expressing the corresponding HC and LC in a 1:1 ratio at a density of 0.8-1.2 million cells/mL in a 1:3 ratio with 1 mg/L PEImax (Polysciences). The recombinant IgG antibodies were isolated from the cell supernatant after five days as described previously (20 ...
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bioRxiv - Neuroscience 2019Quote: ... 1-μm YG beads (Fluoresbrite® YG Microspheres, Calibration Grade 1 μm, #18860; Polysciences, Incorporated, Warrington, PA, USA) were diluted at 4.6 × 108 beads/mL with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Following transfection using polyethyleneimine (Polysciences #24765-1), virus-containing supernatant was collected 48 h later and filtered through a 0.45 μm polyethersulfone filter (VWR 28145-505) ...
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bioRxiv - Genomics 2019Quote: ... or 1 mg/mL polyethylenimine (Polysciences 23966) to DNA (e.g ...
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bioRxiv - Cell Biology 2019Quote: ... using 1 mg/mL polyethylenimine (PEI; Polysciences). The medium was changed 24 hours after transfection ...
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bioRxiv - Plant Biology 2021Quote: ... post-stained 1% aqueous uranyl acetate (Polysciences) and Sato’s triple lead (sodium citrate ...
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bioRxiv - Biophysics 2020Quote: ... A silica bead (Polysciences, 1 mm diameter) was placed on the gel after 200 nm crimson fluorospheres were first gravity-settled on the gel surface to function as markers for measuring bead contact area with epifluorescence microscopy ...
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bioRxiv - Cancer Biology 2022Quote: ... and polyethyleneimine polymer (Polysciences Inc; 23966-1), which was pre-incubated for 15 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... or PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... and polyethylenimine polymer (Polysciences Inc; 23966-1), which had been pre-incubated for 15 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... via Polyethylenimine PEI 25K (Polysciences, 23966-1) and V0 virus were subsequently amplified.
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bioRxiv - Pathology 2021Quote: ... PolyFreeze tissue freezing medium (Polysciences 19636-1) was used to cryopreserve pieces ...
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bioRxiv - Neuroscience 2023Quote: ... using Polyethylenimine (PEI)-Max (24765-1, Polysciences). The culture medium was changed at 16–24 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 0.15 µl Fast Blue (Polysciences # 17740-1) was injected bilaterally in the basolateral amygdala (BLA ...
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bioRxiv - Cancer Biology 2023Quote: ... using polyethyleneimine linear (Polysciences, Cat# 23966-1). After 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µm Fluoresbrite Yellow Green Microspheres (Polysciences) were washed in cell culture media and used as a control for phagocytosis at 100 beads per cell ...
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bioRxiv - Neuroscience 2023Quote: ... each transfected with PEImax (Polysciences #24765-1). Each PEIMax batch was volumetrically titrated at total ugDNA:uLPEI for optimal transfection efficiency.
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bioRxiv - Biophysics 2024Quote: ... Polystyrene beads of 1 µm diameter (Polysciences inc ...
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bioRxiv - Neuroscience 2021Quote: ... coli suspension in LB medium or a 1% (vol/vol) suspension of 1 μm diameter polystyrene beads (Polysciences 431), diluted in NGM buffer to 0.1% vol/vol ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Developmental Biology 2022Quote: ... Live adults were placed in a drop of 1 uL H2O and 1 uL polystyrene microspheres (Polysciences, Inc., #00876) and then immobilized on 6% agarose pads ...
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bioRxiv - Neuroscience 2022Quote: ... Worms were mounted on a 10% agarose pad with 1.6 µl of a 1:1 mixture of polystyrene beads (Polybead, catalog #00876-15, Polysciences) and 30 µM Muscimol (catalog #195336 ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... Cell pellets were infiltrated with a 1:1 mixture of propylene oxide and Polybed 812 epoxy resin (Polysciences, Inc.) for 2 hours and infiltrated overnight ...
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bioRxiv - Bioengineering 2024Quote: ... cells were transfected with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Bioengineering 2024Quote: ... Twenty-four hours after transfection with 0.5 μg of plasmid DNA per well using 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.), cells were incubated in HBSS containing 10 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Bioengineering 2024Quote: ... transfection was carried out using 0.5 μg of plasmid DNA and 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Calculations of relative fluorescence per cell were done by normalizing red chlorophyll fluorescence per cell to 2 μm reference fluorescent beads (catalog no. 18604; Polysciences, Warrington, PA, USA) as previously described [72] ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells were transiently transfected with 2 μg of GPR109A construct together with 5 μg 22F cAMP plasmid using the transfection reagent polyethyleneimine (PEI) linear (Polysciences, Cat. no. 23966) at DNA ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2023Quote: ... After keeping them on ice for no longer than 20 minutes they were fixated for 50 minutes in 2 % paraformaldehyde (PFA, Polysciences, diluted in PBS) while shaking at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Biochemistry 2021Quote: ... and wildtype b0,+AT (the pEZT-C-TwinStrep-mCherry-OaSLC7A9 plasmid) were transfected into HeLa cells at 1:1 molar ratio by using PEI MAX (40,000 MW Linear; Polysciences, Inc.). The ratio of DNA ...
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bioRxiv - Microbiology 2020Quote: ... Infections were performed by spinoculation for 2 hours at 1400 x g with VSVg pseudotyped HIV-1 at MOI of 5 in the presence of 1 μg/ml PEI (Polysciences) and 16 μg/ml Polybrene (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: 8 × 106 HEK 293T cells were transfected with a plasmid encoding PUUV GPC (35 μg in pCAGGS vector) and Tim-1 (3 μg) using PEI (3:1 PEI:DNA, Polysciences, Inc.). After 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... RIKEN BRC) [22] were mixed with 111 µl of 1 mg/ml PEI MAX® (pH7.5) (Cat. No. 24765-1, Polysciences) in Opti-MEM™ I Reduced Serum Medium (Cat ...
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bioRxiv - Immunology 2021Quote: ... HEK293F were grown to a density of ∼1.0 × 106 cells/ml and were co-transfected with heavy chain (HC) and (LC) plasmids (1:1) mixed with PEIMAX (Polysciences). After five days ...
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bioRxiv - Biochemistry 2022Quote: ... The transfection solution was prepared by combining 25 µl (per dish hereafter) of polyethylenimine (PEI) Max solution (1 mg ml-1; Polysciences), 1 ml of Opti- MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... These vectors were transiently transfected into 293F cells at a concentration of 1 million/ml with 1 μg of DNA per million cells using branched polyethylenimine (PEI) (Polysciences). 60-72 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Immunology 2021Quote: ... using PEI Max (1 mg/mL, Polysciences, Inc.) at a 3:1 ratio (PEI Max:DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... Six□micrograms of PEI-Max (Polysciences, #24765-1) were used for transfection ...
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bioRxiv - Microbiology 2021Quote: ... using polyethyleneimine (PEI-max, #24765-1 Polysciences Inc). To measure NS3/4A activity ...
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bioRxiv - Microbiology 2021Quote: ... 1 M anti-digoxygenin coated polystyrene beads (Polysciences) were conjugated to the peptide construct through λ-DNA of the peptide construct by incubation in a buffer containing 10 mM Tris HCl pH 7.5 and 150 mM NaCl for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). For the NKG2D blocking assay ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by fixation with 1% paraformaldehyde (Polysciences, Inc). OT-1 cells were analyzed by flow cytometry using a FACSCanto II cell analyzer (Becton Dickinson ...