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Citations for Polysciences :
201 - 250 of 395 citations for 6 Chloro 3 nitro 2 trifluoromethyl pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... and 48 μL of Polyethylenimine ‘Max’ MW 40,000 Da (Polysciences; 24765-2) per plate ...
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bioRxiv - Physiology 2022Quote: ... C3H10T1/2 cells were transfected with Transporter 5® Transfection Reagent (Polysciences) following manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... with 2 μl of 100 nm polystyrene beads (Cat# 00876-15, Polysciences) and 2 μl of levamisole (from 500 μM stock ...
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bioRxiv - Cancer Biology 2024Quote: ... Warm cross-link reversal buffer (200 µl 800 mM Tris-HCl pH8.0) was added followed by addition of 6 µl of 1:10 Biomag amine paramagnetic beads (48 mg/ ml, Polysciences cat. no. 86001-10). Homogenization was repeated ...
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bioRxiv - Genetics 2024Quote: ... 1 million HEK 293T cells in a single well of a 6-well plate were transfected using PEI-Max MW 40,000 (PolySciences, CAS Number: 49553-93-7) mixed with 1 μg of PsPax2 (Addgene # 12260) ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Biophysics 2022Quote: ... with the density of about 2.5 million cell/ml were transfected using 3 mg plasmid by poly-ethylenimine (PEI, Polysciences) with a 1:3 plasmid to PEI weight ratio ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were pinned onto a Sylgard plate to maintain orientation while incubated overnight at 4°C in 3% glutaraldehyde in 0.1 m□PB (Polysciences). Next ...
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bioRxiv - Immunology 2023Quote: ... One coverslip was activated with 3-aminopropyltriethoxysilane using 0.1 M NaOH (Macron Fine Chemicals) and 0.05% glutaraldehyde (Polysciences Inc.) and the other coated with dichloromethylsilane (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in wash buffer and 10 µl BioMag®Plus Concanavalin A (ConA) beads (Polysciences, 86057-3) were added for 10 min at RT ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 µm diameter or Dynabeads T1: 1 µm diameter) at concentrations of 5×106/µl (high) or 5×105/µl (low) or polystyrene beads (Polysciences, 64090-15: 6 µm diameter) at concentrations of 1×105/µl (high ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transfected with 4 µl of Polyethylenimine (PEI) (Polysciences cat# 23966-2) at 1 mg/ml concentration for every 1 µg plasmid DNA stock in 9ml of Opti-MEM ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with PEI (Polyethylenimine 25kD linear from Polysciences, cat# 23966-2). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfection was performed using polyethylenimine (#23966-2; Polysciences Inc., Warrington, PA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... All transfections were carried out using linear polyethylenimine (PEI; Polysciences cat. #23966-2) by standard protocols.
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bioRxiv - Neuroscience 2021Quote: ... neurons were stained with 1 mL of 2% uranyl acetate (Polysciences #21447-25) in water for 30 min at room temperature and then briefly washed with 50% ethanol (Pharmco #111000200 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg/μL PEI (Polyethylenimine HCl MAX Linear MW 40000 (PolySciences, 24765–2)) and 1 μg/ μL DNA (3:1 ...
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bioRxiv - Biochemistry 2019Quote: ... Transient transfection was performed with Polyethylenimine (PEI, 25 kDa Polysciences Inc. # 23966-2) mixed with DNA in a 3:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... All samples included 2 μm diameter Fluoresbrite beads (Polysciences Inc., Warrington, PA, USA) for size reference and alignment purposes ...
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bioRxiv - Genomics 2022Quote: ... the samples were contrasted by 2% uranylacetate (Polysciences Inc, Cat No 21447-25) in water for 1 minute ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected using polyethylenimine (PEI) (Polysciences, 23966-2, 1 mg/mL) and 3rd generation lentivirus packaging vectors (pMDLg ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of 1 mg/ml linear Polyethylenimine (PEI; Polysciences, Inc, 24765-2) and 1-2 µg of each plasmid ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were fixed with 2% osmium tetroxide in PBS (Polysciences, 23310-10) for membrane preservation ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... HEK cells were transfected using Polyethylenimine (PEI) (1mg/mL) (Polysciences, cat# 23966-2). 3µg plasmid DNA (6-well dishes ...
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bioRxiv - Cell Biology 2023Quote: ... and psPAX.2 (3.75 μg) with PEI MAX 40K (Polysciences Inc., # 24765-100) (1:3 DNA to PEI ratio) ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were transfected with BirAGFP-KIF13B Tail 2 or Tail 3 constructs (designated C2 and C3, respectively, in (Serra-Marques et al., 2020)) using Polyethylenimine (PEI; Mw 2500; Polysciences) at a 3:1 PEI:DNA ratio (w/w) ...
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bioRxiv - Cell Biology 2022Quote: ... six-well plates with about 80-90% confluency were transfected with 1 µg plasmid DNA and 3 µL PEI (Polysciences) per well ...
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bioRxiv - Biochemistry 2019Quote: ... followed by ∼3 microliters of the final assay solution containing 5 ng mL-1 500 nm diameter beads (PolySciences Inc,), coated nonspecifically with neutravidin18(ThermoFisher Inc.).
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bioRxiv - Cell Biology 2019Quote: ... samples were washed 3 times for 30 mins with PBS then incubated through a cryoprotective series of sucrose solutions of increasing concentration from 10-50% for ≥3 hours each before embedding into OCT compound (Tissue Tek) in disposable cryomolds (Polysciences) and frozen in an isopentane bath cooled by liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Sections were washed again 3 times for 20 minutes each time before mounting onto slides with AquaPoly mounting media (18606, Polysciences). Mounted sections were stored at 4°C in dark slide boxes until image processing.
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bioRxiv - Neuroscience 2020Quote: ... Cells were reverse transfected with 3 to 25 ng receptor vector and ~200 ng combined reporter vectors per well using polyethylenimine (Polysciences). Six h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... were seeded in 24-well plates and transfected the following day using PEI MAX (3:1 PEI to DNA ratio; Polysciences) with 500 ng pHIV-1 and 0-250 ng pcDNA3.1 protein expression construct ...
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bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated at 37°C in a 5% CO2 incubator for 3 days and fixed with 10% paraformaldehyde (Polysciences) for 24 hours ...
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bioRxiv - Neuroscience 2019Quote: ... and 1 µm (2 µL at 1.5×107 beads/µL; Polysciences, Cat#: 07310-15) polystyrene microbeads through a 50-100 µm cannula in the cornea formed by a beveled glass micropipette connected by polyethylene tubing to a Hamilton syringe (Hamilton Company Reno ...
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bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed in 2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM cacodylate buffer ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed and fixed with 2% ultra-pure formaldehyde (Polysciences, Warrington, PA) for 20 minutes ...
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bioRxiv - Molecular Biology 2022Quote: A stock solution of 1 mg / ml linear Polyethylenimine (PEI; Polysciences, Inc 24765-2) was prepared in a buffer containing 25 mM Hepes ...