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Citations for Polysciences :
151 - 200 of 841 citations for 6 Chloro 3 methylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... or pαH-SARS-CoV-2 RBD-Fc using 2 mg polyethylenimine (Polysciences, Cat# 24765). 6 days after transfection ...
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bioRxiv - Genetics 2024Quote: ... 6-10 larvae were placed in molds (Peel-A-Way disposable embedding molds, Polysciences) and sucrose solution removed ...
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bioRxiv - Molecular Biology 2019Quote: ... or PEI (POLYSCIENCES 23966-2) into HEK293T cells grown on a 10cm dish (Moffat et al. ...
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bioRxiv - Synthetic Biology 2023Quote: Polyethylenimine (PEI) (Polysciences 23966-2) was used to transfect HEK293FT cells ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent 2 μm beads (Polysciences Fluoresbrite® YG Microspheres 2.00μm 18338-5 ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cells in a single well of 6-well plate (confluency ≥80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Bioengineering 2023Quote: ... MS2 aptamer-modified sgRNA and VSV-G in a ratio of 2:2:2:1 at a dose of 20 µg total plasmid DNA using 50 µg linear 25K MW polyethylenimine (PEI, Polysciences) per T175 flask ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Cell Biology 2020Quote: ... from Bangs Laboratories and Latex polymer 3-micron beads (17134-15) from Polysciences. Murine IFNγ (315-05 ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 15 µl of BioMag ConcanavalinA beads (Polysciences, #86057-3) in binding buffer (20 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Biochemistry 2021Quote: ... ~1.8 mg pCAG-ATP8B1 and ~0.3 mg pCAG-CDC50A/B were incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... A 1 mL sample of exponential phase bacteria was pelleted at 6,000 rcf and resuspended in 1 mL fix (2% paraformaldehyde/2.5% glutaraldehyde (Polysciences Inc., Warrington, PA) in 100 mM sodium cacodylate buffer ...
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bioRxiv - Physiology 2021Quote: ... Cells were plated in 35-mm tissue culture treated dishes and transfected 1-2 days later using polyethylenimine 25k (Polysciences, Inc) with a mass ratio of 1:3 (cDNA:PEI) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293™ cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Molecular Biology 2019Quote: ... linear mw 25,000 (Polysciences 23966-2). Cells were washed with DMEM or DMEM-F12 2-4 hours after transfection reagent was added (Longo et al. ...
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bioRxiv - Cell Biology 2021Quote: ... After fixation with 2% glutaraledhyde (Polysciences) in PBS (pH7.4) ...
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bioRxiv - Bioengineering 2021Quote: ... polystyrene latex microspheres (Polysciences, 18142-2) with our HELP gels ...
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bioRxiv - Bioengineering 2023Quote: ... 2-sulfoethyl methacrylate (PolySciences, Warminster, PA) was added to a final concentration of 5 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were bound to Concanavalin A beads (86057-3, Polysciences Inc), followed by permeabilization using a digitonin containing buffer ...
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bioRxiv - Neuroscience 2022Quote: ... nerves were fixed in 3% glutaraldehyde in 0.1 ml PBS (Polysciences) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... N-(3-Aminopropyl) methacrylamide hydrochloride (APMA, >98%) was obtained from Polysciences Inc ...
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bioRxiv - Neuroscience 2023Quote: ... 250.000 cells were captured with BioMagPlus Concanavalin A (Polysciences, 86057-3) and incubated with the appropriate primary antibody overnight at 4°C (anti-Histone H3(tri methyl K4 ...
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bioRxiv - Immunology 2024Quote: ... 10uL/reaction of concanavalin A-coated beads (Cat #86057-3, Polysciences) were equilibrated with Binding Buffer (20mM HEPES ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 μl of BioMag Plus Concanavalin A beads (Polysciences # 86057-3) were washed twice in 500 μL of Binding Buffer (20 mM HEPES-KOH pH 7.9 ...
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bioRxiv - Neuroscience 2021Quote: ... Four retrograde tracers were employed for a total of 29 injections: (i) cholera toxin subunit B (CTB; Polysciences Inc, Eppelheim, Germany), (ii ...
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bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
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bioRxiv - Neuroscience 2021Quote: ... cells were transfected with 1 μg of plasmid using 2 μg of Polyethylenimine Max (PEI MAX) transfection reagent (Polysciences, Warrington, PA, USA), and were seeded onto either glass bottom dishes or glass bottom chambers (Matsunami ...
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bioRxiv - Neuroscience 2021Quote: ... 293T media was replaced with 18 mL fresh media and the cells were transfected using the polyethylenimine (PEI) (1 mg/mL, Polysciences #23966-2) transfection method ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc., Germany) per mL culture volume ...
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bioRxiv - Bioengineering 2022Quote: PEG-αMA hydrogels were fabricated at 17.5 wt% with a 0.375 molar ratio of 70:30 DTT:MMP2-degradable as described above and swollen overnight in PBS with 2.2 mM LAP and 10 µM methacryloxyethyl thiocarbamoyl rhodamine B (Polysciences Inc., Warrington, PA). Swollen hydrogels were exposed to 10 mW cm−2 365-nm light for five minutes through a chrome-on-quartz photomask with spatially patterned opaque and transparent regions ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...
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bioRxiv - Biophysics 2021Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg polyethylenimines (PEIs, Polysciences) in 50 mL SMM 293T-II medium (Sino Biological Inc. ...
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bioRxiv - Cell Biology 2021Quote: Latex NH2-beads (3 μm diameter, 3.5 x 108 beads/preparation, Polysciences) were activated with 8% glutaraldehyde in 0.5 ml PBS for 120 min under rotation at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... was covalently functionalized with N-(3-Aminopropyl)methacrylamide (APMAm; Polysciences, Warrington, PA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC ...
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bioRxiv - Developmental Biology 2023Quote: Aliquot 15 μL of Concanavalin A (ConA) beads (Polysciences cat#86057-3) and bind to magnet for 5 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 mg of PEI “MAX” (Polysciences) (40 kDa ...
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bioRxiv - Genetics 2020Quote: ... and PEI Max (Polysciences, Inc. 24765-2). At 72 hr posttransfection ...
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bioRxiv - Bioengineering 2021Quote: ... 2-aminoethyl methacrylate (AEMA, 3.04 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
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bioRxiv - Immunology 2022Quote: ... 100 μL of 2% paraformaldehyde solution (Polysciences, #18814-20 ...
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bioRxiv - Immunology 2022Quote: ... The light and heavy chain expression plasmids of LH01 or anti-PD-L1 were mixed at 2:1 and co-transfected using liner polyethylenimine (PEI) with a molecular weight of 25 kDa (Polysciences, Warrington, PA, USA). LH02 was produced by transfecting HEK293E cells with Fc-Sushi-IL15-expression plasmid alone ...
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bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...