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Citations for Polysciences :
51 - 100 of 839 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl polyethylenimine (PEI) 25K (1mg/ml; Polysciences, 23966-1) per μg of plasmid DNA were mixed with pre-warmed Opti-MEM media (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... We then sterilized 1- and 6-µm diameter polystyrene microbeads (Polysciences, Cat#: 07310-15 and 07312-5) as noted in Cone et al. ...
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bioRxiv - Microbiology 2022Quote: ... 6 μm polystyrene microspheres (Polysciences, USA) were used to calibrate the flow cytometer and evaluate cell size ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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bioRxiv - Microbiology 2023Quote: ... one day before plasmid transfection with polyethylenimine (PEI, Polysciences, Inc. ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... 1 μg of plasmid and 2 μl of 40 kD linear polyethyleneimine (1 mg/ml, Polysciences, 24765-1) were used per milliliter of cells ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA (1 mg mL-1) was mixed with linear 25 kDa polyethyleneimine (2 mg mL-1; Polysciences) and co-transfected in OptiPRO SFM medium (4% final concentration ...
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bioRxiv - Microbiology 2021Quote: Pseudoviruses bearing the wild-type or variant HIV-1 Envs were produced by co-transfecting the Env-expressing pSVIIIenv plasmid and the luciferase-encoding pNL4-3.Luc.R-E-vector (NIH HIV Reagent Program) at a 1:1 ratio into HEK293T cells using polyethylenimine (Polysciences). The cell supernatants containing pseudoviruses were harvested 48 hr later ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Immunology 2022Quote: ... and co-transfected with a 1:3 ratio of the two HC/LC DNA plasmids and 1 mg/l PEImax (Polysciences). Five days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... Co- transfection of those plasmids were performed at a density of 4-5 million cells/mL in a 1:3 ratio (weight: weight) of plasmids to PEImax at 1 mg/ml (Polysciences). Following a 4-day incubation ...
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bioRxiv - Neuroscience 2024Quote: ... in DMEM complete medium as described previously17 with the following modifications: a 3:1 ratio of 1 µg/µl polyethenylamine (PEI) (Linear, MW 25,000, Polysciences, 23966) to transfer DNA was used in place of TransIT Lenti Transfection Reagent ...
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bioRxiv - Biochemistry 2020Quote: ... using 1 μg of plasmid DNA and 3 μg of linear polyethyleneimine (PEI; Polysciences, Germany). Cells were harvested 6 days after transfection by centrifuging the medium for 15 minutes at 1000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated the cells for 35-36 hours at 37°C before preparation of slides for confocal microscopy ...
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bioRxiv - Molecular Biology 2023Quote: ... and transient transfection was achieved by using a 3:1 ratio of PEI (Polysciences, Inc.) to DNA in Opti-MEM (Invitrogen).
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bioRxiv - Immunology 2023Quote: Polybead amino 6 µm-diameter microspheres (Polysciences) were prepared to covalently couple rCD6 or rICAM1 ...
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bioRxiv - Genetics 2020Quote: ... Transient transfection of the 3 µg ml-1 of vector DNA plus 9 µg ml-1 Polyethylenimine (PEI) (25 kDa linear PEI, Polysciences, Inc.) was used to express the protein in 2.5 × 106 cells ml-1 ...
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bioRxiv - Microbiology 2021Quote: ... were transiently transfected at a cell density of 1 × 106 cells/mL using a 1:3 ratio of plasmid DNA and polyethylenimine (PEI 25K™, Polysciences). Cells were incubated at 37°C for 7 days in a humidified incubator containing 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... cells were transfected at a cell density of 1 million mL-1 using a mixture of 1 μg of recombinant expression plasmid and 3 μg of polyethyleneimine (PEI; Polysciences, Germany). Cultures were supplemented with 0.6% Primatone RL (Merck ...
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bioRxiv - Bioengineering 2021Quote: ... 1 uL (or 1.5 uL for target-binding experiments) of 1 mg/mL PEI (Polysciences, 24765-2) dissolved in water was added to DNA in media and vortexed for 10 seconds ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 10 mins incubation with 1 mL of 2 mg/mL Polyethylenimine (PEI) (Polysciences, POL23966-1). As next step ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with 1 μg ml-1 DNA using 2-fold excess of linear polyethylenimine (PEI; Polysciences), pre-incubated for 15 min in Optimem medium (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... washed again 3 times in DPBS and 2 times in ddH2O and finally mounted in Aqua Polymount medium (cat#: 18606, Polysciences, Warrington, PA, USA). For immunofluorescence assays using antibodies from different host species ...
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bioRxiv - Molecular Biology 2021Quote: ... NIH3T3 cells were transfected with 1 μg/ml plasmid with polyethyleneimine (3 μg/ml; Polysciences, 23966). Media was changed the next day and cells harvested 48 hrs later ...
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bioRxiv - Systems Biology 2023Quote: ... cells were transfected with 1 µg of the pTwist_CMV plasmid using 3 µg of PEI (Polysciences) as a transfection reagent or with with 1 µg of the pDEST_GFP plasmid (Control) ...
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bioRxiv - Neuroscience 2019Quote: ... One hundred μL of 0.1% chitosan solution (Polysciences, Valley Road, Warrington) prepared in 1% acetic acid (pH 5 ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ug pHelper plasmid into one 15-cm dish of confluent HEK-293T cells using PEI-Max (Polysciences Inc. catalog # 24765-1). After transfection the next day we changed the medium to 1% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... 200 μL of 6% paraformaldehyde (Polysciences 18814-10) in PBS was added to each well and incubated for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were stained with 1 mL of 2% uranyl acetate (Polysciences #21447-25) in water for 30 min at room temperature and then briefly washed with 50% ethanol (Pharmco #111000200 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg/μL PEI (Polyethylenimine HCl MAX Linear MW 40000 (PolySciences, 24765–2)) and 1 μg/ μL DNA (3:1 ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transfected using polyethylenimine (PEI) (Polysciences, 23966-2, 1 mg/mL) and 3rd generation lentivirus packaging vectors (pMDLg ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of 1 mg/ml linear Polyethylenimine (PEI; Polysciences, Inc, 24765-2) and 1-2 µg of each plasmid ...
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bioRxiv - Biophysics 2019Quote: ... 1 μL of the PB including 3% fluorescent microbeads (ex:441, em:486, 1.0 μm, Polysciences, Inc.) was spread at the same time with the KI cells ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...
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bioRxiv - Bioengineering 2024Quote: ... 3 µl of polyethylenimine (Polysciences) was diluted in 50 µl Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid transfections were performed with 1 μg of DNA per single well of a 6-well plate using PEI MAX (Polysciences, INC.) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and gRNA expression plasmid (6 μg) were co-transfected with transfection reagent at a 2.6/1 ratio of Polyethylenimine (PEI; Polysciences, Warrington, USA) (μg)/total DNA (μg) ...
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bioRxiv - Neuroscience 2019Quote: ... and 1 µm (2 µL at 1.5×107 beads/µL; Polysciences, Cat#: 07310-15) polystyrene microbeads through a 50-100 µm cannula in the cornea formed by a beveled glass micropipette connected by polyethylene tubing to a Hamilton syringe (Hamilton Company Reno ...
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bioRxiv - Molecular Biology 2022Quote: A stock solution of 1 mg / ml linear Polyethylenimine (PEI; Polysciences, Inc 24765-2) was prepared in a buffer containing 25 mM Hepes ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were picked up onto 1×2 mm formvar coated copper slot grids (Polysciences), and further stained with uranyl acetate followed by lead citrate ...
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bioRxiv - Microbiology 2021Quote: ... HEK293F cells grown in 10 ml of medium (3.0 x 106 cells/ml) were transfected with 6 µg of pCAGGS-NP (1-395) using Polyethyleneimine MAX (Polysciences, Warrington, PA, USA). Three days post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 293T cells grown to 70-80% confluency were transfected with 6 μg ITR-containing plasmid and 22 μg pDGM6 using 112 μg acidified PEI (Polysciences, 23966-1). 48-72 hours after transfection ...
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bioRxiv - Molecular Biology 2020Quote: One anterior chamber was injected with Polybead Microspheres (Polysciences, Inc., Warrington, PA, USA), consisting of 2 μL of 6 μm diameter beads ...
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bioRxiv - Biophysics 2021Quote: ... carboxylated 3 µm silica beads (Polysciences) were coated with a mixture of biotinylated pentameric FN7-10 (a four-domain segment of fibronectin responsible for cell binding and containing the RGD and PHSRN motifs70 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 µm polystyrene beads (Polysciences) suspended in 1X PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The cells at 1.5*106 confluence were transiently transfected with 1: 3 ratio of desired DNA and polyethylenimine (PEI) (Polysciences) complex and incubated at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... we dispensed 6 μm diameter polystyrene beads (Polybead microspheres, Polysciences) diluted in HBSS into recording μ-slide channels and let them settle to the bottom of the μ-slide ...